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Nerve injury site filling material and preparation method thereof

A technology of filling material and nerve injury, applied in prosthetics, tissue regeneration, medical science, etc., can solve the problems of blocking the directional growth of microangiogenesis nerve axons, slow healing of nerve injury sites, inflammation and fibrosis, etc., to ensure slow Release and long-acting effect, good immune microenvironment, guaranteed non-loss effect

Active Publication Date: 2021-02-26
WENZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The applicant found that the existing filling materials for nerve injury sites have strong immunogenicity, which can easily cause inflammation and fibrosis at the site of nerve injury. Blocking the formation of microvessels and the directional growth of nerve axons, resulting in slow healing of the nerve injury site

Method used

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  • Nerve injury site filling material and preparation method thereof
  • Nerve injury site filling material and preparation method thereof
  • Nerve injury site filling material and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1 Preparation of nerve injury site filling material

[0022] Preparation method 1: According to the composition of each experimental group of nerve injury site filling materials in Table 1, weigh each component, crush the nerve decellularized matrix according to the aseptic method, and mix with heparin containing heparin high-affinity cell growth factor - the poloxamer gel is mixed evenly, and the aminoated bletilla striata polysaccharide-glyceraldehyde gel is added, mixed evenly to form a uniform semi-solid gel.

[0023] Preparation method 2: According to the composition of each experimental group of the nerve injury site filling material in Table 1, weigh each component, crush the nerve decellularized matrix according to the aseptic method, and mix it with the aminoated Bletilla striata polysaccharide-glyceraldehyde gel, Add heparin-poloxamer gel loaded with heparin high-affinity cell growth factor, and mix well to form a uniform semi-solid gel.

[0024] T...

Embodiment 2

[0032] Example 2 In vivo evaluation of the application effect of filling materials at nerve injury sites in each group

[0033] (1) Rat central nervous system injury model

[0034] After the 220-250g SD female rats were checked for paralysis and inconvenient movement, they were anesthetized with pentobarbital sodium 30mg / Kg. After the anesthesia took effect, intramuscular injection of cefazoline sodium (according to 5-10mg / 100g), the back was shaved. Hair and prone positions were fixed, and the skin was disinfected with povidone iodine; rats were placed in the prone position, fixed on the rat spinal process fixation frame, the chest and back were shaved and the skin was prepared, and routine disinfection was carried out. Taking T9 as the center, make a midline incision on the back with a length of about 3 cm. Cut the skin and muscle to expose the 8th to 9th thoracic vertebrae (T 8 -T 9 ) and the 9th to 10th thoracic vertebrae (T 9 -T 10 ), bite off T 9 and part T 9 -T ...

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Abstract

The invention provides a nerve injured site filling material. The filling material is prepared from aminated bletilla striata polysaccharide-glyceraldehyde gel, heparin high-affinity cytokine entrapped heparin-poloxamer gel and acellular nerve matrix, and the mechanical strength and degrading speed of the filling material can be controlled by changing a component proportion. The nerve injured sitefilling material can overcome the defect of high immunogenicity in the prior art, has low immunogenicity, and has multiple functions including nerve conduction, neural induction and mechanical support, provides an optimized physicochemical and biological microenvironment, can effectively guide directional growth of nerve axons, has excellent mechanical performance, and realizes quick repair of the nerve injured site.

Description

technical field [0001] The invention relates to a nerve injury site filling material, in particular to the composition, preparation and application of a nerve injury site filling material. Background technique [0002] Nerve injury caused by various reasons is a common disease of the nervous system, including spinal cord injury, peripheral nerve injury, etc., which will lead to sensorimotor disorders and nutritional disorders in the innervated area, seriously affecting the quality of life of patients. For severe spinal cord injury or peripheral nerve injury, there is currently no effective clinical treatment. [0003] Studies have shown that some cell growth factors can promote the repair of damaged nerves and restore the signal transmission between the nerve injury site and surrounding nerves. However, these cell growth factors have short in vivo half-life and poor biomembrane penetration ability. No matter systemic or local administration, there are defects such as low dr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/18A61L27/20A61L27/36A61L27/52A61L27/54
CPCA61L27/18A61L27/20A61L27/3604A61L27/52A61L27/54A61L2300/414A61L2300/604A61L2430/32C08L71/02C08L5/00
Inventor 赵应征鲁翠涛肖健金冰慧沈碧欣朱群燕童梦琪
Owner WENZHOU MEDICAL UNIV