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Application of berberine to preparation of sensibilization medicament for tumor radiotherapy

A tumor radiation and berberine technology is applied in the field of tumor radiation therapy sensitizing drugs, which can solve the problems of toxicity, inability to produce anti-tumor effects, and no reports on the application of berberine radiation sensitizing drugs, so as to reduce toxic side effects and improve treatment. effect, the effect of reducing the radiation dose

Inactive Publication Date: 2019-03-01
王军
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Studies have also found that large doses of berberine have anti-tumor effects, can inhibit the proliferation of colon cancer cells, inhibit the growth of esophageal cancer, breast cancer and prostate cancer cells, but high doses of berberine will also cause toxicity, although reducing the dose can eliminate toxicity , but the antitumor effect cannot be produced when the dose is small
At the same time, there is no report on the application of berberine as a radiosensitizer.

Method used

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  • Application of berberine to preparation of sensibilization medicament for tumor radiotherapy
  • Application of berberine to preparation of sensibilization medicament for tumor radiotherapy
  • Application of berberine to preparation of sensibilization medicament for tumor radiotherapy

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Embodiment

[0034] (1) Materials: Human breast cancer cell lines MDA-MB-231 and MCF-7 were purchased from the American Cell Collection Center (ATCC), and were preserved and cultivated by the inventor's laboratory; D-MEM medium dry powder, calf serum , trypsin powder was purchased from Gibco; standard protein molecular weight, SDS-PAGE loading buffer, RIPA protein lysate, TE electrophoresis buffer, 10× transfer solution, 30% Acry-Bis, Tris-Hcl, ammonium persulfate (AP), SDS, tetramethylethylenediamine (TEMED), propidium iodide (PI) were purchased from Jiangsu Beyantian Biotechnology Research Institute; dimethyl sulfoxide (DMSO), agarose and Tween-20 were from Shanghai High-tech Bioengineering Co., Ltd.; caspase-3, bcl-2, bax, cyclin D1 and other antibodies were purchased from Santa Cruzs Biotechnology Company of the United States; RNase A and 10 μg / mL proteinase K were purchased from Sigma Aldrich Company of the United States.

[0035] (2) Cell culture: MDA-MB-231 and MCF-7 cells were cult...

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Abstract

The invention discloses a sensibilization medicament for tumor radiotherapy. In the sensibilization medicament for the tumor radiotherapy, berberine is taken as an active component. As proved by the research, (1) when used for breast cancer cell treatment, the berberine can induce blocking and apoptosis of dose-dependent cells in a G0 / G1 period; (2) the berberine remarkably lowers the expression levels of cell cycle regulation related protein cyclin B and anti-apoptosis protein Bcl-2, and improves the expression levels of apoptin Bax and caspase-3; (3) the sensibility of breast cancer cells treated by the berberine to the radiotherapy is improved remarkably. By adopting the berberine, the prepared sensibilization medicament for the tumor radiotherapy has the functions of improving the tumor radiotherapy effect, lowering the irradiation dose of radiotherapy for the tumor patients, and lowering the toxic and side effects caused by the radiotherapy, so that the quality of life of the patients is improved.

Description

technical field [0001] The invention belongs to the field of sensitizing drugs for tumor radiotherapy, and in particular relates to the application of berberine in the preparation of sensitizing drugs for tumor radiotherapy. Background technique [0002] In the prior art, commonly used radiotherapy sensitizing drugs include: [0003] (1) MISO (Misonodaxole): MISO is a fully affirmed hypoxic cell sensitizer in the clinical trial stage. [0004] (2) Kanglaite: It is an injection made from the traditional Chinese medicine coix seed. The drug has been widely used in the treatment of various malignant tumors, but also a radiosensitizer. [0005] (3) SR-2508 (Etanidacole): SR-2508 is an amide derivative of nitroimidazole. Its sensitizing potency is similar to that of MISO, but its toxicity is lower. The maximum tolerable total dose is about 3 times that of MISO. SR-2508 also has a sensitizing effect on chemotherapeutic drugs. [0006] (4) AK-2123: AK-2123 is a nitroimidazole c...

Claims

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Application Information

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IPC IPC(8): A61K31/4375A61P35/00
CPCA61K31/4375A61P35/00
Inventor 王军张可芬艾惠如
Owner 王军