A method for simultaneous detection of six synthetic sweeteners in tea
A technology for sweeteners and tea leaves, applied in the field of chemical analysis, can solve problems such as difficulty in application, complex tea matrix components, and inability to measure synthetic sweeteners at the same time, achieving simple operation, avoiding multiple extraction operations, and good purification effect. Effect
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Embodiment 1
[0038] 1) Sample preparation
[0039] The tea samples were pulverized into powder by a pulverizer.
[0040] 2) Sample pretreatment
[0041] Accurately weigh 2.00 g of tea samples into a 50 mL stoppered centrifuge tube, add 20 mL of acetonitrile / water (1:1, v / v), shake on a shaker for 30 minutes, and centrifuge at 4700 rpm for 5 minutes. Pipette 1.00 mL of the supernatant to a 2 mL centrifuge tube containing 200 mg of graphitized carbon black or 50 mg of multi-walled carbon nanotubes or 100 mg of graphitized carbon black mixed with 25 mg of multi-walled carbon nanotubes, vortex for 1 minute, and centrifuge at 15,000 rpm for 5 Minutes, take 1 mL of the supernatant and pass it through a nylon filter (13 mm, 0.22 μm), and the resulting filtrate is used as a sample solution for analysis by liquid chromatography-tandem mass spectrometry.
[0042] 3) Standard solution preparation
[0043] A blank sample solution was obtained by using a blank tea sample that does not contain 6 targ...
Embodiment 2
[0054] Addition and recovery tests were carried out on the detection methods using 3 different dispersive solid phase extraction adsorbents. Green tea and black tea were used as substrates respectively, and the addition levels of 6 synthetic sweeteners were 0.1mg / kg, 1mg / kg and 10mg / kg. .
[0055] The results of the addition recovery test are shown in Table 3. When graphitized carbon black was used as dispersive SPE adsorbent, the average recoveries of the six synthetic sweeteners in green tea were 92.9%-106.9%, and the relative standard deviations were all less than 3.8%; The average recoveries were 84.4%-99.5%, and the relative standard deviations were all less than 4.4%. When multi-walled carbon nanotubes were used as dispersive SPE adsorbents, the average recoveries of the six synthetic sweeteners in green tea were 89.5%-99.9%, and the relative standard deviations were all less than 4.6%; the six synthetic sweeteners in black tea The average recoveries were 86.2%-102.2%,...
Embodiment 3
[0060] To verify the linear relationship of 6 synthetic sweeteners using 3 different dispersive SPE sorbents, the prepared concentrations were 0.0005mg / L, 0.001mg / L, 0.005mg / L, 0.01mg / L, 0.05 mg / L, 0.1mg / L, 0.5mg / L, 1mg / L green tea and black tea matrix matching standard solution, with 1 / c s Fit a linear standard curve to the weights. The concentration in the sample at which the converted signal-to-noise ratio was 10 was the quantitation limit of the method.
[0061] The linear relationship and limit of quantification of the standard curve are shown in Table 4. Within the corresponding linear range, the linear correlation coefficients of the six sweeteners in green tea and black tea were all greater than or equal to 0.9990, and the three methods had good linear relationships. When graphitized carbon black is used as the dispersive SPE adsorbent, the quantitative limits of cyclamate, saccharin sodium, acesulfame potassium, aspartame, alitame and neotame in green tea and black ...
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