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Preparation method of triterpenic acid in jujube material

A technology of triterpene acids and jujube materials, which is applied in the field of preparation of triterpene acids in jujube materials, can solve the problems of low extraction rate and low separation purity of active ingredients, and achieve high separation efficiency

Active Publication Date: 2019-03-22
HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But its extraction rate is low, and the separation purity of active ingredients is low

Method used

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  • Preparation method of triterpenic acid in jujube material
  • Preparation method of triterpenic acid in jujube material
  • Preparation method of triterpenic acid in jujube material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 A preparation method of triterpene acids in jujube powder

[0035] The preparation method is carried out in the following order of steps:

[0036] (1) Preparation of jujube powder

[0037] The jujube fruit is pitted and crushed to obtain the jujube powder;

[0038] (2) Preparation of jujube crude extract

[0039] The jujube powder was extracted with 80% ethanol solution under reflux at 85°C, extracted twice for 2 hours each time, the extract was concentrated under reduced pressure, and the ethanol solution was recovered to obtain the crude jujube extract;

[0040] (3) Preparation of triterpene acid macroporous resin separation components

[0041] Jujube crude extract was separated by AB-8 macroporous adsorption resin column, the volume ratio of sample volume to separation material was 1:200, and the separation steps were as follows: first, wash with pure water until the separation column was colorless, and then use 2 column volumes of The 70% ethanol solut...

Embodiment 2

[0046] Example 2 A preparation method of triterpene acids in jujube dregs

[0047] The preparation method is carried out in the following order of steps:

[0048] (1) Preparation of jujube dregs

[0049] The jujube fruit is pitted and crushed to obtain jujube powder, and an extraction solvent is added. The weight volume ratio of the jujube powder to the extraction solvent is 1:10, and the extraction is carried out under heating and pressure. The extraction solvent is 15% ethanol aqueous solution, and the extraction temperature is 80°C. Extract for 20 min each time, repeat the extraction twice, and filter to obtain jujube dregs;

[0050] (2) Preparation of jujube crude extract

[0051] Jujube dregs were reflux extracted with absolute ethanol at 80°C, extracted twice for 90 min each time, the extract was concentrated under reduced pressure, and ethanol was recovered to obtain crude jujube extract;

[0052] (3) Preparation of triterpene acid macroporous resin separation compon...

Embodiment 3～8

[0058] The preparation method of triterpene acid in the jujube material of embodiment 3~8

[0059] Examples 3 to 8 are respectively a preparation method of triterpene acids in jujube material. The operation steps are the same as those in Example 1 and Example 2, except for the type and purity of the alcohol, and the reaction time and reaction time in the process. The temperature and other control parameters are different, see the table below for details:

[0060]

[0061]

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PUM

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Abstract

The invention discloses a preparation method of triterpenic acid in a jujube material. According to the preparation method of the triterpenic acid in the jujube material, by using alcohols as an extracting agent and alcohol-acid as an eluant, the jujube material is heated, refluxed and extracted; the purity of the triterpenic acid obtained by using two separation methods of macroporous absorbent resin column chromatography and preparative liquid chromatography is high, the content of the triterpenic acid exceeds 90%, and main components are betulinic acid, ursolic acid and oleanolic acid. In the preparation process, organic solvents are all recycled, so that the preparation method of the triterpenic acid in the jujube material is economic and environmentally friendly. The preparation method of the triterpenic acid in the jujube material, disclosed by the invention, has the advantages that AB-8 or HPD100 adsorption resin is adopted, the AB-8 adsorption resin and the HPD100 adsorption resin are polystyrene low polar or nonpolar polymers, the service life is relatively long, and the triterpenic acid elution efficiency is relatively high. The preparation method of the triterpenic acidin the jujube material, disclosed by the invention, has the advantages that the preparative liquid chromatography is a maximum technical characteristic different from the characteristic of the conventional technology, and online monitoring and targeted separation are adopted, so that the separation efficiency is high.

Description

technical field [0001] The invention belongs to the technical field of natural product extraction, in particular to a preparation method of triterpene acids in jujube materials. Background technique [0002] Jujube is a plant of the genus Jujube in the family Rhamnaceae, which has a wide planting area in my country. Its fruit-jujube is a popular dried fruit among the people, and its total output accounts for the first among dried fruits. Jujube is sweet and delicious, rich in nutrition. It can be eaten and used as medicine. It has many medicinal functions such as nourishing yin and kidney, strengthening the body, softening blood vessels, appetizing and invigorating the spleen, etc., and is known as the "king of all fruits". [0003] In recent years, with the development of jujube processing industry, a lot of production waste jujube dregs have also been brought. Studies have found that jujube dregs contain a certain amount of triterpene acids. Triterpene acids are a gener...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/725A61P35/00A61P43/00
CPCA61K36/725A61K2236/333A61K2236/39A61K2236/51A61K2236/55A61P35/00A61P43/00
Inventor 李存满罗晓田晶
Owner HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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