Fermented traditional Chinese medicine preparation and its preparation method and application
A technology of traditional Chinese medicine preparations and Chinese herbal medicines, applied in the directions of application, pharmaceutical formulations, drug combinations, etc., can solve the problems such as the inability to guarantee the efficacy and quality stability of Chinese herbal medicines, the low level of modernization of traditional Chinese medicine, and the slow pace of internationalization.
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Embodiment 1
[0048] A kind of fermented traditional Chinese medicine preparation and preparation method thereof
[0049] 1. Strains: Lactobacillus acidophilus.
[0050] 2. Mixed powder of compound Chinese herbal medicine: weigh 1 part of Codonopsis pilosula, 2 parts of Rehmannia glutinosa and 10 parts of soybean meal in parts by weight, dry Rehmannia glutinosa in an oven at 50°C-55°C for 3-4 hours, and mix with Codonopsis pilosula, Rehmannia glutinosa, After the soybean meal is mixed, it is pulverized and passed through a 60-mesh sieve to obtain a mixed powder, which is set aside.
[0051] 3. Medium formula
[0052] Activation medium: tryptone 10g, beef powder 5g, yeast powder 4g, glucose 20g, Tween 80 1mL, dimethyl hydrogen phosphate heptahydrate 2g, sodium acetate trihydrate 5g, triammonium citrate 2g, magnesium sulfate heptahydrate 0.2g , manganese sulfate tetrahydrate 0.05g, distilled water 1000mL, agar 15g, pH value 6.2.
[0053] Expansion medium: tryptone 10g, beef powder 5g, yeas...
Embodiment 2
[0069] Example 2 is basically the same as Example 1, except that the amount of mixed powder added to the fermentation medium and Chinese herbal medicines and the fermentation conditions are different, specifically:
[0070] The fermentation medium is: wheat bran 8g, peptone 10g, beef powder 6g, yeast powder 3g, glucose 12g, Tween 801.3mL, dipotassium hydrogen phosphate 2.5g, sodium acetate 6, triammonium citrate 3g, magnesium sulfate 0.2g, 0.05g of manganese sulfate, 1000mL of pure water; 12g of mixed powder of Chinese herbal medicine was added to 1L of fermentation medium.
[0071] The fermentation conditions are: temperature 38°C, pressure 0.06kPa, pH value 6.6, stirring speed 30r / min, culture time 38h.
Embodiment 3
[0073] Example 3 is basically the same as Example 1, except that the amount of mixed powder added to the fermentation medium and Chinese herbal medicines and the fermentation conditions are different, specifically:
[0074] The fermentation medium is: wheat bran 10g, peptone 8g, beef powder 6g, yeast powder 6g, glucose 13g, Tween 801.2mL, dipotassium hydrogen phosphate 2g, sodium acetate 5, triammonium citrate 2.5g, magnesium sulfate 0.18g, 0.08g of manganese sulfate, 1000mL of pure water; 16g of mixed powder of Chinese herbal medicine was added to 1L of fermentation medium.
[0075] The fermentation conditions are: temperature 36°C, pressure 0.05kpa, pH 6.4, stirring speed 30r / min, culture time 48h.
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