Preparation method and application of metal-oxide nanometer hyacinth-bletilla-tuber earthworm complex

A technology of earthworm protein and oxide, which is applied in the direction of drug combination, hydrolyzed protein components, peptide/protein components, etc., can solve the problems of high economic cost, large consumption, and difficulty in commercial production, so as to promote recovery and reduce oxidation loss effect

Inactive Publication Date: 2019-03-29
SHAANXI UNIV OF CHINESE MEDICINE
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Problems solved by technology

However, as a biomacromolecule, earthworm protein is very prone to protein degradation, and there are problems such as large consumption and high economic costs in the actual use process.
Bletilla striata has the effects of

Method used

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  • Preparation method and application of metal-oxide nanometer hyacinth-bletilla-tuber earthworm complex
  • Preparation method and application of metal-oxide nanometer hyacinth-bletilla-tuber earthworm complex
  • Preparation method and application of metal-oxide nanometer hyacinth-bletilla-tuber earthworm complex

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preparation example Construction

[0024] The embodiment of the present embodiment is a method for preparing metal oxide nano-Bletilla striata dilong protein complex, comprising the following steps:

[0025] S1, preparation of earthworm stress protein:

[0026] Wash the fresh earthworm (Essium chinensis) with 0.9% NaCl solution for several times to remove the dirt on the body surface, and place the cleaned earthworm in physiological saline solution with an air pump continuous oxygen supply system at 25°C Let it spit out the sediment in the body for 4 hours; remove the surface moisture with absorbent paper and transfer it to a beaker; add 10% sucrose of the total weight of earthworm and quickly stir it evenly with a glass rod, wait for 10 minutes and collect the water in the beaker The yellow-green liquid is the earthworm stress protein, add 1X anti-protein degradation enzyme, and then add NaN with a concentration of 0.02% 3 Obtain the earthworm stress protein liquid as a preservative, and store it for future u...

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Abstract

The invention discloses a preparation method and application of a metal-oxide nanometer hyacinth-bletilla-tuber earthworm complex. The preparation method of the metal-oxide nanometer hyacinth-bletilla-tuber earthworm complex comprises the following steps: preparing earthworm stress proteins; preparing layered metal oxide nanometer carriers; and then, preparing the metal-oxide nanometer hyacinth-bletilla-tuber earthworm complex. The metal-oxide nanometer hyacinth-bletilla-tuber earthworm complex is applied for repairing craniocerebral injury of rats. Compared with the prior art, the metal-oxidenanometer hyacinth-bletilla-tuber earthworm complex loads the earthworm stress proteins and active ingredients of the traditional Chinese medicine hyacinth bletilla tubers in the layered metal oxidenanometer carriers in self assembly; and thus, the prepared complex is a sustained-release and economical medical-purpose pharmaceutical preparation. The metal-oxide nanometer hyacinth-bletilla-tuberearthworm complex has preserved efficacy, as well as reduced degradation of proteins and oxidation loss of traditional Chinese medicine ingredients, thereby achieving the purpose of promoting craniocerebral injury recovery.

Description

technical field [0001] The invention relates to the technical field of preparation of animal protein and plant medicinal material nanometer traditional Chinese medicine compound, in particular to a preparation method and application of metal oxide nanometer bletilla striata protein compound. Background technique [0002] Brain injury is a common injury in modern society, especially in traffic accident cases. The impact of external objects on the brain severely disrupts the normal function of the brain, resulting in temporary or permanent loss of cognitive, physical and psychological functions. Traumatic brain injury is currently the leading cause of death and disability in people under the age of 45. The traditional Chinese medicine terrestrial protein has been proven to effectively promote the proliferation and differentiation of wound tissue cells, and accelerate the ability of wound healing. However, as a biomacromolecule, ground dragon protein is very prone to protein ...

Claims

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Application Information

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IPC IPC(8): A61K38/01A61K47/69A61P25/00A61P25/28A61K36/898A61K35/62
CPCA61K35/62A61K36/898A61K38/012A61K47/6953A61P25/00A61P25/28A61K2300/00
Inventor 王东谷婷权珍妮王瑞辉王雪静张晓叶
Owner SHAANXI UNIV OF CHINESE MEDICINE
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