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Novel electrochemical biological sensor for detecting Alpha2, 6 sialyl gylcan

A biosensor, electrochemical technology, applied in the direction of material electrochemical variables, scientific instruments, instruments, etc., can solve expensive problems, achieve the effect of fewer detection steps, improve sensitivity, and accelerate electron transfer

Active Publication Date: 2019-03-29
CHONGQING MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these methods require expensive equipment and materials, and the tested samples must undergo complex pretreatment to eliminate other interferences. In addition, they must be analyzed by technicians in specialized laboratories

Method used

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  • Novel electrochemical biological sensor for detecting Alpha2, 6 sialyl gylcan
  • Novel electrochemical biological sensor for detecting Alpha2, 6 sialyl gylcan
  • Novel electrochemical biological sensor for detecting Alpha2, 6 sialyl gylcan

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Experimental program
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Embodiment 1

[0037] Step 1. Dispense 1mL of 2.5mg mL -1 The solution of carboxylated single-walled carbon nanohorns (c-SWCNHs) was sonicated for 5 min; then, 5 mL of potassium chloroplatinate (K 2 PtCl 4 ) and 6 mL of 0.1 mM methylene blue (MB) were heated and stirred at 90° C. for 5 hours. Finally, the mixture was centrifuged at 10,000 rpm for 5 minutes, washed three times, dissolved in 1 mL of ultrapure water, and finally dried in a vacuum oven for use.

[0038] Step 2. Use 0.3 and 0.05 μm Al respectively 2 o 3 Polish the electrode into a mirror surface with powder, then ultrasonically ultrasonicate the electrode for 5 minutes each in the order of ultrapure water, absolute ethanol, and ultrapure water, and dry at room temperature for later use;

[0039] Step 3. Add 10 μL of electrode modification material gold nanorods-streptavidin (AuNRs-SA) dropwise on the electrode surface, and dry at room temperature;

[0040] Step 4. Dissolve 8 μL of bone-synthetic black wood protein (bio-SNA) ...

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Abstract

The invention successfully develops a specificity hypersensitivity sandwiched electrochemical immune sensor on the basis of a novel gold nanorod-streptavidin (AuNPs-SA) composite material and carboxylsingle-wall nanometer angle-sulfur-doped platinum (c-SWCNHs / S-PtNC) and is used for detecting Alpha2, 6 sialyl gylcan (Alpha2, 6-sial-Gs) in human serum. By the gold nanorod-streptavidin (AuNPs-SA),the conductivity of an electrode is improved, electron transfer is improved, and meanwhile, an active site is provided for an immobilization biotin-labeled black elderberry (bio-SNA) by the streptavidin and a biotin system. Moreover, the c-SWCNHs / S-PtNC has excellent catalytic performance on reduction of H2O2, and triamido phenylboronic acid (M-APBA) can be effectively captured by Pt-NH2 and NH-CO-. The sensor has the advantages of wide linear range, high sensitivity, high specificity and favorable reusability and is rapid to detect, and the sensor can be used for measuring Alpha2, 6-sial-Gs content in human serum and has huge potential in clinical test.

Description

Technical field: [0001] The invention relates to a preparation method and application of an electrochemical immunosensor for quantitative detection of α2,6 sialylated glycans in clinical practice, especially based on gold nanorod-streptavidin nanoparticle composite material and carboxylated single-walled carbon The nanohorn-sulfur-doped platinum nanocomposite material is used as a biosensor prepared as a signal probe for detecting α2, 6 sialoglycan, belonging to the field of electrochemical detection. Background technique: [0002] α2,6-sialylated glycan (α2,6-sial-Gs) is a broad-spectrum tumor marker, which is closely related to malignant behaviors such as tumor proliferation, metastasis, spread and tumor antigenicity. During the apoptosis process of cancer cells, α2,6-sial-Gs is shed under the hydrolysis of glycosidase and released into the blood circulation, so that the level of α2,6-sial-Gs in serum is significantly increased. Therefore, the detection of α2,6-sial-Gs ha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26G01N27/30G01N27/327G01N33/531
CPCG01N27/26G01N27/30G01N27/3278G01N33/531
Inventor 于超何俊琳李佳
Owner CHONGQING MEDICAL UNIVERSITY
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