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Method for preparing hydrogel ink applicable to 3D (three-dimensional) biological printing

A bioprinting and hydrogel technology, which is applied in applications, inks, household appliances, etc., can solve the problems of poor structural stability of hydrogel inks, and achieve the goal of enriching biological applications, improving structural stability, and realizing functional modification Effect

Active Publication Date: 2019-04-05
XIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a preparation method of hydrogel ink suitable for 3D bioprinting, which solves the problem of poor structural stability of existing hydrogel ink

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0049] The preparation method of methacryloyl chitosan, specifically:

[0050] Dissolve chitosan in acetic acid aqueous solution, stir evenly, add methacrylic anhydride, and then place it at 0-4°C for 4h-6h reaction to obtain methacrylated polysaccharide solution, and then add methacryl Put the purified polysaccharide solution into a dialysis bag with a molecular weight of 3000-7000 and dialyze for 5-7 days, then freeze-dry at -55-60°C for 48-72 hours, and finally freeze-dry at -18-25°C Refrigerate, reserve;

[0051] The mass ratio of chitosan, acetic acid aqueous solution and methacrylic anhydride is 1:100:1~2;

[0052] In the acetic acid aqueous solution, the volume ratio of acetic acid to water is 1:50-100;

[0053] The preparation method of hydroxybutyl chitosan, specifically:

[0054] Disperse the chitosan powder in NaOH aqueous solution with a mass fraction of 45-55% for alkalization treatment, stir at room temperature for 24-36 hours under nitrogen protection, filter...

Embodiment 1

[0078] A method for preparing a hydrogel ink suitable for 3D bioprinting, specifically implemented according to the following steps:

[0079] Step 1. Dissolve methylpropionylated chitosan in high-sugar DMEM cell culture medium, and freeze it in an ice-water bath at 0°C for 15 minutes, then add propylene F127 to the cell culture medium to obtain a mixed solution, and then Then the mixed solution is centrifuged to separate the solid from the liquid, and the obtained solid is refrigerated at 4°C for later use;

[0080] The mass ratio of methylpropionylated chitosan, high-sugar DMEM cell culture medium, and propylene F127 is 2:100:20;

[0081] The centrifugation rate is 500rpm, and the centrifugation time is 3min;

[0082] The preparation method of methacryloyl chitosan, specifically:

[0083] Dissolve chitosan in aqueous acetic acid solution, stir evenly, add methacrylic anhydride, and then react at 0°C for 4 hours to obtain a methacrylated polysaccharide solution, and then mak...

Embodiment 2

[0100] A method for preparing a hydrogel ink suitable for 3D bioprinting, specifically implemented according to the following steps:

[0101] Step 1. Dissolve hydroxyethyl chitosan in low-sugar DMEM cell culture medium, and freeze it in an ice-water bath at 1°C for 20 minutes, then add aminated F127 to the cell culture medium to obtain a mixed solution, and then mix the The liquid is treated by centrifugation to separate the solid from the liquid, and the obtained solid matter is refrigerated at 4°C for later use;

[0102] The mass ratio of hydroxyethyl chitosan, cell culture medium and aminated F127 is 5:100:25;

[0103] The centrifugation rate is 800rpm, and the centrifugation time is 3min;

[0104] Among them, the manufacturer of hydroxyethyl chitosan is Wuhan Yuancheng Gongchuang Technology Co., Ltd., the degree of substitution of hydroxyethyl chitosan is 85%, and the viscosity is 400mPa·s;

[0105] The preparation method of aminated F127 is specifically:

[0106] Mix F...

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Abstract

The invention discloses a method for preparing hydrogel ink applicable to 3D (three-dimensional) biological printing. The method is particularly implemented according to steps of dissolving chitosan derivatives in cell culture media; freezing the cell culture media under ice-water bath conditions; adding Pluronic F127 derivatives into the cell culture media; carrying out centrifugation; carrying out refrigeration; dissolving natural polysaccharides in deionized water to obtain liquid; adding sodium periodate and ethylene glycol into the liquid; stirring the sodium periodate, the ethylene glycol and the liquid under dark conditions; carrying out dialysis; carrying out freeze drying; carrying out refrigeration and then dissolving substances in cell culture media; ultimately immersing hydrogel precursors in polysaccharide cross-linking agent culture medium solution; carrying out stable cross-linking to obtain the hydrogel ink. The method has the advantages that reaction on chitosan or thederivatives of the chitosan and natural polysaccharide cross-linking agents is utilized, accordingly, the structural stability of the hydrogel ink can be improved, polysaccharide molecules can be functionally modified, and the biological application of the hydrogel ink can be enriched.

Description

technical field [0001] The invention belongs to the technical field of preparation of hydrogel ink materials, and in particular relates to a preparation method of hydrogel ink suitable for 3D bioprinting. Background technique [0002] 3D bioprinting is an important technical means to use the principle of additive manufacturing to position and assemble biological materials and cell units, and to manufacture tissue engineering scaffolds and tissues and organs. The key core of 3D bioprinting is to print ink materials. The "hydrogel" scaffold is similar to the extracellular matrix structure of organisms and can load cells in situ. It is an ideal 3D printing bioink. 3D printing hydrogel bio-ink has four basic features: (1) Adjustable viscosity; viscosity is the resistance to the flow of hydrogel precursor sol, and adjustable viscosity is suitable for designing the printing method, printing parameter range and delay time. Structural deformation is of great significance; (2) Excel...

Claims

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Application Information

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IPC IPC(8): C09D11/14C09D11/03C08B37/08B33Y70/00
CPCB33Y70/00C08B37/003C09D11/03C09D11/14
Inventor 任鹏刚张华杨帆任芳靳彦岭
Owner XIAN UNIV OF TECH
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