Method for preparing hydrogel ink applicable to 3D (three-dimensional) biological printing

A bioprinting and hydrogel technology, which is applied in applications, inks, household appliances, etc., can solve the problems of poor structural stability of hydrogel inks, and achieve the goal of enriching biological applications, improving structural stability, and realizing functional modification Effect

Active Publication Date: 2019-04-05
XIAN UNIV OF TECH
View PDF8 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a preparation method of hydrogel ink suitab

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0049] The preparation method of methacryloyl chitosan is specifically as follows:

[0050] Dissolve chitosan in aqueous acetic acid, stir well, add methacrylic anhydride, and then react at 0~4℃ for 4h~6h to obtain methacryloyl polysaccharide solution. The polysaccharide solution is put into a dialysis bag with a molecular weight of 3000-7000 for 5-7 days, then freeze-dried at -55℃~-60℃ for 48~72h, and finally at -18~-25℃ Refrigerate and reserve;

[0051] The mass ratio of chitosan, acetic acid aqueous solution and methacrylic anhydride is 1:100:1~2;

[0052] In the acetic acid aqueous solution, the volume ratio of acetic acid to water is 1:50-100;

[0053] The preparation method of hydroxybutyl chitosan is specifically as follows:

[0054] Disperse the chitosan powder in a NaOH aqueous solution with a mass fraction of 45 to 55% for alkalization. Stir at room temperature for 24 to 36 hours under nitrogen protection, filter, add isopropanol and stir for 24 to 36 hours to make the alkal...

Example Embodiment

[0077] Example 1

[0078] A preparation method of hydrogel ink suitable for 3D bioprinting, specifically implemented according to the following steps:

[0079] Step 1. Dissolve methyl propionylated chitosan in high-sugar DMEM cell culture medium, and freeze it in an ice-water bath at 0°C for 15 minutes, then add acrylic F127 to the cell culture medium to obtain a mixed solution. The mixed liquid is centrifuged to separate the solid and liquid, and the obtained solid material is refrigerated at 4°C for later use;

[0080] The mass ratio of methpropionyl chitosan, high-sugar DMEM cell culture medium, and acrylic F127 is 2:100:20;

[0081] The centrifugal speed is 500rpm, and the centrifugal time is 3min;

[0082] The preparation method of methacryloyl chitosan is specifically as follows:

[0083] Dissolve chitosan in acetic acid aqueous solution, stir well, add methacrylic anhydride, and then react at 0℃ for 4h to obtain a methacrylylated polysaccharide solution, and then methacrylylated ...

Example Embodiment

[0099] Example 2

[0100] A preparation method of hydrogel ink suitable for 3D bioprinting, specifically implemented according to the following steps:

[0101] Step 1. Dissolve hydroxyethyl chitosan in low-sugar DMEM cell culture medium and freeze it in an ice-water bath at 1°C for 20 minutes, then add aminated F127 to the cell culture medium to obtain a mixed solution, and then mix it The liquid is centrifuged to separate the solid and liquid, and the obtained solid material is refrigerated at 4°C for later use;

[0102] The mass ratio of hydroxyethyl chitosan, cell culture medium, and aminated F127 is 5:100:25;

[0103] The centrifugal speed is 800rpm, and the centrifugal time is 3min;

[0104] Among them, the manufacturer of hydroxyethyl chitosan is Wuhan Yuancheng Gongchuang Technology Co., Ltd., the degree of hydroxyethyl substitution is 85%, and the viscosity is 400mPa·s;

[0105] The preparation method of aminated F127 is specifically:

[0106] Mix F127, N'N-carbonyldiimidazole (C...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Viscosityaaaaaaaaaa
Viscosityaaaaaaaaaa
Login to view more

Abstract

The invention discloses a method for preparing hydrogel ink applicable to 3D (three-dimensional) biological printing. The method is particularly implemented according to steps of dissolving chitosan derivatives in cell culture media; freezing the cell culture media under ice-water bath conditions; adding Pluronic F127 derivatives into the cell culture media; carrying out centrifugation; carrying out refrigeration; dissolving natural polysaccharides in deionized water to obtain liquid; adding sodium periodate and ethylene glycol into the liquid; stirring the sodium periodate, the ethylene glycol and the liquid under dark conditions; carrying out dialysis; carrying out freeze drying; carrying out refrigeration and then dissolving substances in cell culture media; ultimately immersing hydrogel precursors in polysaccharide cross-linking agent culture medium solution; carrying out stable cross-linking to obtain the hydrogel ink. The method has the advantages that reaction on chitosan or thederivatives of the chitosan and natural polysaccharide cross-linking agents is utilized, accordingly, the structural stability of the hydrogel ink can be improved, polysaccharide molecules can be functionally modified, and the biological application of the hydrogel ink can be enriched.

Description

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Owner XIAN UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2023 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap