Method for preparing hydrogel ink applicable to 3D (three-dimensional) biological printing

A bioprinting and hydrogel technology, which is applied in applications, inks, household appliances, etc., can solve the problems of poor structural stability of hydrogel inks, and achieve the goal of enriching biological applications, improving structural stability, and realizing functional modification Effect

Active Publication Date: 2019-04-05
XIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a preparation method of hydrogel ink suitab

Method used

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Examples

Experimental program
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Example Embodiment

[0049] The preparation method of methacryloyl chitosan is specifically as follows:

[0050] Dissolve chitosan in aqueous acetic acid, stir well, add methacrylic anhydride, and then react at 0~4℃ for 4h~6h to obtain methacryloyl polysaccharide solution. The polysaccharide solution is put into a dialysis bag with a molecular weight of 3000-7000 for 5-7 days, then freeze-dried at -55℃~-60℃ for 48~72h, and finally at -18~-25℃ Refrigerate and reserve;

[0051] The mass ratio of chitosan, acetic acid aqueous solution and methacrylic anhydride is 1:100:1~2;

[0052] In the acetic acid aqueous solution, the volume ratio of acetic acid to water is 1:50-100;

[0053] The preparation method of hydroxybutyl chitosan is specifically as follows:

[0054] Disperse the chitosan powder in a NaOH aqueous solution with a mass fraction of 45 to 55% for alkalization. Stir at room temperature for 24 to 36 hours under nitrogen protection, filter, add isopropanol and stir for 24 to 36 hours to make the alkal...

Example Embodiment

[0077] Example 1

[0078] A preparation method of hydrogel ink suitable for 3D bioprinting, specifically implemented according to the following steps:

[0079] Step 1. Dissolve methyl propionylated chitosan in high-sugar DMEM cell culture medium, and freeze it in an ice-water bath at 0°C for 15 minutes, then add acrylic F127 to the cell culture medium to obtain a mixed solution. The mixed liquid is centrifuged to separate the solid and liquid, and the obtained solid material is refrigerated at 4°C for later use;

[0080] The mass ratio of methpropionyl chitosan, high-sugar DMEM cell culture medium, and acrylic F127 is 2:100:20;

[0081] The centrifugal speed is 500rpm, and the centrifugal time is 3min;

[0082] The preparation method of methacryloyl chitosan is specifically as follows:

[0083] Dissolve chitosan in acetic acid aqueous solution, stir well, add methacrylic anhydride, and then react at 0℃ for 4h to obtain a methacrylylated polysaccharide solution, and then methacrylylated ...

Example Embodiment

[0099] Example 2

[0100] A preparation method of hydrogel ink suitable for 3D bioprinting, specifically implemented according to the following steps:

[0101] Step 1. Dissolve hydroxyethyl chitosan in low-sugar DMEM cell culture medium and freeze it in an ice-water bath at 1°C for 20 minutes, then add aminated F127 to the cell culture medium to obtain a mixed solution, and then mix it The liquid is centrifuged to separate the solid and liquid, and the obtained solid material is refrigerated at 4°C for later use;

[0102] The mass ratio of hydroxyethyl chitosan, cell culture medium, and aminated F127 is 5:100:25;

[0103] The centrifugal speed is 800rpm, and the centrifugal time is 3min;

[0104] Among them, the manufacturer of hydroxyethyl chitosan is Wuhan Yuancheng Gongchuang Technology Co., Ltd., the degree of hydroxyethyl substitution is 85%, and the viscosity is 400mPa·s;

[0105] The preparation method of aminated F127 is specifically:

[0106] Mix F127, N'N-carbonyldiimidazole (C...

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Abstract

The invention discloses a method for preparing hydrogel ink applicable to 3D (three-dimensional) biological printing. The method is particularly implemented according to steps of dissolving chitosan derivatives in cell culture media; freezing the cell culture media under ice-water bath conditions; adding Pluronic F127 derivatives into the cell culture media; carrying out centrifugation; carrying out refrigeration; dissolving natural polysaccharides in deionized water to obtain liquid; adding sodium periodate and ethylene glycol into the liquid; stirring the sodium periodate, the ethylene glycol and the liquid under dark conditions; carrying out dialysis; carrying out freeze drying; carrying out refrigeration and then dissolving substances in cell culture media; ultimately immersing hydrogel precursors in polysaccharide cross-linking agent culture medium solution; carrying out stable cross-linking to obtain the hydrogel ink. The method has the advantages that reaction on chitosan or thederivatives of the chitosan and natural polysaccharide cross-linking agents is utilized, accordingly, the structural stability of the hydrogel ink can be improved, polysaccharide molecules can be functionally modified, and the biological application of the hydrogel ink can be enriched.

Description

technical field [0001] The invention belongs to the technical field of preparation of hydrogel ink materials, and in particular relates to a preparation method of hydrogel ink suitable for 3D bioprinting. Background technique [0002] 3D bioprinting is an important technical means to use the principle of additive manufacturing to position and assemble biological materials and cell units, and to manufacture tissue engineering scaffolds and tissues and organs. The key core of 3D bioprinting is to print ink materials. The "hydrogel" scaffold is similar to the extracellular matrix structure of organisms and can load cells in situ. It is an ideal 3D printing bioink. 3D printing hydrogel bio-ink has four basic features: (1) Adjustable viscosity; viscosity is the resistance to the flow of hydrogel precursor sol, and adjustable viscosity is suitable for designing the printing method, printing parameter range and delay time. Structural deformation is of great significance; (2) Excel...

Claims

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Application Information

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IPC IPC(8): C09D11/14C09D11/03C08B37/08B33Y70/00
CPCB33Y70/00C08B37/003C09D11/03C09D11/14
Inventor 任鹏刚张华杨帆任芳靳彦岭
Owner XIAN UNIV OF TECH
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