Method for preparing hydrogel ink applicable to 3D (three-dimensional) biological printing
A bioprinting and hydrogel technology, which is applied in applications, inks, household appliances, etc., can solve the problems of poor structural stability of hydrogel inks, and achieve the goal of enriching biological applications, improving structural stability, and realizing functional modification Effect
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[0049] The preparation method of methacryloyl chitosan is specifically as follows:
[0050] Dissolve chitosan in aqueous acetic acid, stir well, add methacrylic anhydride, and then react at 0~4℃ for 4h~6h to obtain methacryloyl polysaccharide solution. The polysaccharide solution is put into a dialysis bag with a molecular weight of 3000-7000 for 5-7 days, then freeze-dried at -55℃~-60℃ for 48~72h, and finally at -18~-25℃ Refrigerate and reserve;
[0051] The mass ratio of chitosan, acetic acid aqueous solution and methacrylic anhydride is 1:100:1~2;
[0052] In the acetic acid aqueous solution, the volume ratio of acetic acid to water is 1:50-100;
[0053] The preparation method of hydroxybutyl chitosan is specifically as follows:
[0054] Disperse the chitosan powder in a NaOH aqueous solution with a mass fraction of 45 to 55% for alkalization. Stir at room temperature for 24 to 36 hours under nitrogen protection, filter, add isopropanol and stir for 24 to 36 hours to make the alkal...
Example Embodiment
[0077] Example 1
[0078] A preparation method of hydrogel ink suitable for 3D bioprinting, specifically implemented according to the following steps:
[0079] Step 1. Dissolve methyl propionylated chitosan in high-sugar DMEM cell culture medium, and freeze it in an ice-water bath at 0°C for 15 minutes, then add acrylic F127 to the cell culture medium to obtain a mixed solution. The mixed liquid is centrifuged to separate the solid and liquid, and the obtained solid material is refrigerated at 4°C for later use;
[0080] The mass ratio of methpropionyl chitosan, high-sugar DMEM cell culture medium, and acrylic F127 is 2:100:20;
[0081] The centrifugal speed is 500rpm, and the centrifugal time is 3min;
[0082] The preparation method of methacryloyl chitosan is specifically as follows:
[0083] Dissolve chitosan in acetic acid aqueous solution, stir well, add methacrylic anhydride, and then react at 0℃ for 4h to obtain a methacrylylated polysaccharide solution, and then methacrylylated ...
Example Embodiment
[0099] Example 2
[0100] A preparation method of hydrogel ink suitable for 3D bioprinting, specifically implemented according to the following steps:
[0101] Step 1. Dissolve hydroxyethyl chitosan in low-sugar DMEM cell culture medium and freeze it in an ice-water bath at 1°C for 20 minutes, then add aminated F127 to the cell culture medium to obtain a mixed solution, and then mix it The liquid is centrifuged to separate the solid and liquid, and the obtained solid material is refrigerated at 4°C for later use;
[0102] The mass ratio of hydroxyethyl chitosan, cell culture medium, and aminated F127 is 5:100:25;
[0103] The centrifugal speed is 800rpm, and the centrifugal time is 3min;
[0104] Among them, the manufacturer of hydroxyethyl chitosan is Wuhan Yuancheng Gongchuang Technology Co., Ltd., the degree of hydroxyethyl substitution is 85%, and the viscosity is 400mPa·s;
[0105] The preparation method of aminated F127 is specifically:
[0106] Mix F127, N'N-carbonyldiimidazole (C...
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