A kind of fermentation medium for producing doramectin and the production method of doramectin

A technology of fermentation medium and production method, which is applied in the field of biopharmaceuticals, can solve problems such as unsatisfactory potency, and achieve the effects of accelerating growth metabolism, simple production method, and improving fermentation level

Active Publication Date: 2022-01-04
NEW FOUNDER HLDG DEV LLC +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The potency of this method is still not very satisfactory

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Adopt above-mentioned fermentation step to produce doramectin, wherein:

[0053] (1) Add 0.01g / L of 2,2-methylbutyric acid to the medium at all levels;

[0054] (2) Starch in the fermentation medium is not liquefied separately, all materials are sterilized together, and the concentration of reducing sugar in the medium after sterilization is 1.3%;

[0055] (3) The bacterial concentration of the metabolic stage in the fermentation process is maintained at 44-45%;

[0056] (4) When the fermenter was cultivated for 20 hours, the precursor was fed into the fermenter, and the concentration of the precursor in the fermented liquid was controlled to be 0.1-0.4g / L;

[0057] (5) From the 11th day, add liquefied starch with a mass fraction of 0.5% every day;

[0058] After 370 hours of fermentation, put it into the tank, and the unit of the tank is 2969 μg / ml.

Embodiment 2

[0060] Adopt above-mentioned fermentation step to produce doramectin, wherein:

[0061] (1) Add 0.02g / L of 2,2-methylbutyric acid to the medium at all levels;

[0062] (2) Starch in the fermentation medium is not liquefied separately, all materials are sterilized together, and the concentration of reducing sugar in the medium after sterilization is 1.2%;

[0063] (3) The bacterial concentration of the metabolic stage in the fermentation process is maintained at 45-46%;

[0064] (4) When the fermenter was cultivated for 20 hours, the precursor was fed into the fermenter, and the concentration of the precursor in the fermented liquid was controlled to be 0.1-0.4g / L;

[0065] (5) From the 11th day, add 0.5% liquefied starch every day;

[0066] After 370 hours of fermentation, put it into the tank, and the unit of the tank is 3083 μg / ml.

Embodiment 3

[0068] Adopt above-mentioned fermentation step to produce doramectin, wherein:

[0069] (1) Add 0.03g / L of 2,2-methylbutyric acid to the culture medium at all levels;

[0070] (2) Starch in the fermentation medium is not liquefied separately, all materials are sterilized together, and the concentration of reducing sugar in the medium after sterilization is 1.3%;

[0071] (3) During the fermentation process, the bacterial concentration in the metabolic stage is maintained at 46-47%;

[0072] (4) When the fermenter was cultivated for 20 hours, the precursor was fed into the fermenter, and the concentration of the precursor in the fermented liquid was controlled to be 0.1-0.4g / L;

[0073] (5) From the 11th day, add 0.5% liquefied starch every day;

[0074] After 370 hours of fermentation, put it into the tank, and the unit of the tank is 3468 μg / ml.

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PUM

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Abstract

The invention relates to a fermentation medium for producing doramectin and a production method of doramectin. The composition of the fermentation medium includes: starch 100-140g / L, amylase 0.1-0.2g / L, soybean cake powder 12-18g / L, cottonseed cake powder 12-18g / L, yeast powder 0.5-1.5g / L , 2,2-methylbutyric acid 0.01-0.2g / L, cyclohexanecarboxylic acid or sodium cyclohexanecarboxylic acid 0.2-0.4g / L. The present invention significantly improves the fermentation level of doramectin by improving the fermentation medium and the production method of doramectin. After 370 hours of fermentation, the titer reaches 3545 μg / ml, and the whole production process is simple and easy to operate, which is beneficial to industrial production .

Description

technical field [0001] The invention belongs to the technical field of biopharmaceuticals, and in particular relates to a fermentation medium for producing doramectin and a production method of doramectin. Background technique [0002] Doramectin (DRM), is a new generation of macrolide antiparasitic drugs, through the genetic recombination of Streptomyces avermitilis strainNEAU1069 new strains in the fermentation process with cyclohexane carboxylic acid (cyclohexanec arboxylic acid (CHC) is a kind of abamectin antibiotic produced as a precursor. Because of its wide range of anti-parasites, remarkable effect, easy administration route, high bioavailability, and long drug residual effect, doramectin has been clinically used in veterinary medicine for cattle, horses, sheep, goats, pigs, camels, mammals such as dogs. [0003] At present, the production of doramectin is mainly carried out through biological fermentation. CN107723325A discloses a method for fermenting and produ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P19/62C12R1/465
CPCC12N1/20C12P19/62
Inventor 向中南张葵周镪杨超
Owner NEW FOUNDER HLDG DEV LLC
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