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Copper ion electrochemical sensor and preparation method and application thereof

A copper ion, sensor technology, applied in analytical chemistry, life science and medical fields, can solve the problem of no regeneration, and it is difficult to obtain information on the continuous change of Cu2+ level.

Active Publication Date: 2019-04-05
HUNAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these are disposable electrochemical sensors without the ability to regenerate, and it is difficult to obtain Cu in vivo during pathological events. 2+ Levels of constantly changing information

Method used

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  • Copper ion electrochemical sensor and preparation method and application thereof
  • Copper ion electrochemical sensor and preparation method and application thereof
  • Copper ion electrochemical sensor and preparation method and application thereof

Examples

Experimental program
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preparation example Construction

[0054] figure 2 For the preparation process of copper ion electrochemical sensor of the present invention, comprise the following steps:

[0055] Step 1. Using dendritic polyethyleneimine as a renewable recognition unit, it is covalently modified onto GC electrode 1 by stepwise chemical reaction, and labeled as GC / Cys / Au / MPA / hPEI electrode;

[0056] Step 2, using 6-(ferrocene)hexathiol (FcHT) as a built-in reference unit, adopting a stepwise chemical reaction to covalently modify it onto the GC electrode 2, labeled as GC / Cys / Au / FcHT electrode;

[0057] Step 3: The electrodes prepared in Step 1 and Step 2 are used together to form a reproducible ratiometric copper ion electrochemical sensor.

[0058] image 3 It is a schematic diagram of the equipment connection of the online microdialysis living body sampling-electrochemical sensor system of the present invention. The peristaltic pump is loaded with an airtight syringe to transmit the artificial cerebrospinal solution, and ...

Embodiment 1

[0065] The preparation of GC / Cys / Au electrodes using glassy carbon sheets includes the following steps:

[0066] 1) The bare double glassy carbon block electrode (including GC electrode 1 and GC electrode 2) was polished with 0.05 μm alumina on a polishing cloth, and placed in acetone, nitric acid (1:1, v / v), hydrogen Sodium oxide (50%, w / w) and distilled water were sonicated for 5 minutes in sequence;

[0067] 2) Put the electrode prepared in step 1) in 0.5M H 2 SO 4 Use cyclic voltammetry to scan until the current is stable, the scanning voltage ranges from -1V to +1V, the scanning speed is 0.1V / s, and rinse with distilled water;

[0068] 3) Soak the electrode prepared in step 2) in 10mM cysteine, 10mM EDC, and 10mM NHS solution for 2 hours in sequence, and wash with distilled water;

[0069] 4) Soak the electrode prepared in step 3) in the gold nanoparticle solution synthesized with trisodium citrate for 2 hours, and wash with distilled water to obtain GC1 / Cys / Au electro...

Embodiment 2

[0072] Using the GC1 / Cys / Au electrode obtained in Example 1 to prepare a GC1 / Cys / Au / MPA / hPEI electrode comprises the following steps:

[0073] 5) Soak the GC1 / Cys / Au electrode in 10mM mercaptopropionic acid solution for 2h, and wash it with distilled water;

[0074]6) Soak the prepared electrode in 1 mM dendrimer polyethyleneimine, 10 mM EDC, and 10 mM NHS solution for 2 hours, and wash it with distilled water.

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Abstract

The invention discloses a copper ion electrochemical sensor and a preparation method and application thereof and belongs to the fields of analytical chemistry and life science. The electrochemical sensor is composed of a working electrode modified by branched polyethyleneimine and an internal reference unit modified by mercapto ferrocene, wherein the working electrode modified by branched polyethyleneimine is used as an identification unit of Cu<2+>. According to the copper ion electrochemical sensor and the preparation method and application thereof, the copper ion electrochemical sensor is reproducible and high in sensitivity and selectivity, and online monitoring of the Cu<2+> level in a brain can be realized; the reproducible ratio-type electrochemical sensor is prepared by the adoption of the identification unit, namely branched polyethyleneimine and the internal reference unit, namely mercapto ferrocene; and combined with a microdialysis in-vivo sampling technology, the sensor can realize online regeneration-detection circulation. Moreover, the sensor has good sensitivity, selectivity and stability on Cu<2+> detection and is successfully applied to continuous determination ofthe Cu<2+> level in the brain of a rat in the process of a cerebral ischemia / reperfusion event.

Description

technical field [0001] The invention belongs to the technical fields of analytical chemistry, life science and medicine, and specifically relates to a copper ion electrochemical sensor and its preparation method and application. Background technique [0002] Cu 2+ It is one of the essential micronutrients for living organisms and plays a vital role in physiological and pathological events. Cu 2+ It is a key cofactor of various metalloenzymes, which plays a role in generating cellular energy, reducing molecular oxygen, and activating signal transduction. Cu + / Cu 2+ The imbalance of β can lead to the generation of reactive oxygen species closely related to Alzheimer's disease, Parkinson's disease and other serious neurodegenerative diseases. Accumulating evidence shows that cerebral ischemia is one of the leading causes of death and neurodegenerative diseases in the elderly. Therefore, the development of efficient Cu 2+ Cu 2+ level is of great significance. [0003] ...

Claims

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Application Information

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IPC IPC(8): G01N27/48G01N27/327
CPCG01N27/327G01N27/48
Inventor 谷慧陈述张杰刘灿军侯琪蔡雨杰刘宇
Owner HUNAN UNIV OF SCI & TECH
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