Zinc protoporphyrin nano-crystals and preparation method and applications thereof

A technology of zinc porphyrin and nanocrystals, which is applied in the field of nanomaterials, can solve the problems of zinc porphyrin, and achieve the effects of high drug loading, high stability, high-efficiency photoacoustic imaging of tumors and photodynamic therapy

Inactive Publication Date: 2019-04-12
THE SECOND PEOPLES HOSPITAL OF SHENZHEN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0010] Aiming at the deficiencies of the prior art, the object of the present invention is to provide a method for preparing zinc porphyrin nanocrystals, so as to solve the insoluble problem of zinc porphyrin and improve the stability of the contrast agent in the blood system

Method used

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  • Zinc protoporphyrin nano-crystals and preparation method and applications thereof
  • Zinc protoporphyrin nano-crystals and preparation method and applications thereof
  • Zinc protoporphyrin nano-crystals and preparation method and applications thereof

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Experimental program
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Effect test

Embodiment 1

[0066] The method for preparing zinc porphyrin nanocrystals in this embodiment comprises the following steps:

[0067] The crystallization process of zinc porphyrin is limited in space by using the emulsion system as a template, so as to realize the preparation of zinc porphyrin nanocrystals. The specific steps are as follows:

[0068] (1) Dichloromethane having dissolved zinc porphyrin is used as the oil phase of the emulsion, and with 1.0wt% poloxamer F127 and 0.5wt% dipalmitoylphosphatidylethanolamine-polyethylene glycol-folic acid as surfactant Ultrapure water is used as the water phase, the two phases are mixed, and the power is 100W for 1min to obtain zinc porphyrin oil-in-water emulsion;

[0069] (2) The zinc porphyrin oil-in-water emulsion is dispersed into a larger volume of water phase (1:20), and the power is 100W, and the ultrasonic wave is mixed for 2 minutes to obtain the zinc porphyrin oil-in-water emulsion of the large water phase;

[0070] (3) liquid nitrogen...

Embodiment 2

[0075] The method for preparing zinc porphyrin nanocrystals in this embodiment comprises the following steps:

[0076] The crystallization process of zinc porphyrin is limited in space by using the emulsion system as a template, so as to realize the preparation of zinc porphyrin nanocrystals. The specific steps are as follows:

[0077] (1) Dichloromethane that has dissolved zinc porphyrin is used as the oil phase of the emulsion, and with 2.0wt% poloxamer F127 and 1wt% dipalmitoylphosphatidylethanolamine-polyethylene glycol-folic acid as surfactant super Pure water is used as the water phase, the two phases are mixed, and the power is 200W for 0.5min to obtain zinc porphyrin oil-in-water emulsion;

[0078] (2) The zinc porphyrin oil-in-water emulsion is dispersed into a larger volume of water phase (1:15), and the power is 100W, and the ultrasonic wave is mixed for 2 minutes to obtain the zinc porphyrin oil-in-water emulsion of the large water phase;

[0079] (3) liquid nitro...

Embodiment 3

[0082] The method for preparing zinc porphyrin nanocrystals in this embodiment comprises the following steps:

[0083] The crystallization process of zinc porphyrin is limited in space by using the emulsion system as a template, so as to realize the preparation of zinc porphyrin nanocrystals. The specific steps are as follows:

[0084] (1) Dichloromethane having dissolved zinc porphyrin is used as the oil phase of the emulsion, and with 0.5wt% poloxamer F127 and 0.2wt% dipalmitoylphosphatidylethanolamine-polyethylene glycol-folic acid as surfactant Ultrapure water is used as the water phase, the two phases are mixed, and the power is 50W for 1min to obtain zinc porphyrin oil-in-water emulsion;

[0085] (2) The zinc porphyrin oil-in-water emulsion is dispersed into a larger volume of water phase (1:20), and the power is 180W, and the ultrasonic wave is mixed for 1 min to obtain the zinc porphyrin oil-in-water emulsion of the large water phase;

[0086] (3) liquid nitrogen rapi...

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Abstract

The invention provides zinc protoporphyrin nano-crystals and a preparation method and applications thereof. The preparation method comprises the following steps: subjecting a zinc porphyrin oil-in-water emulsion to rapid freezing in liquid nitrogen and then vacuum freeze-drying so as to obtain zinc protoporphyrin nano-crystals. The preparation method provided by the invention prepares the nano-crystals with an ultra-small particle size and protection of surface hydrophilic polymers, solves the problem of poor solubility of zinc protoporphyrin, and can improve the stability of the zinc protoporphyrin as a contrast agent in the blood system. At the same time, the tumor targeting molecule folic acid is introduced onto the surface of nano-crystals during the preparation process so as to improve the endocytosis ability of tumor cells to zinc protoporphyrin, to realize high-efficiency tumor photoacoustic imaging and photodynamic therapy, and to have good application prospect and applicationvalue.

Description

technical field [0001] The invention belongs to the field of nanometer materials, and relates to a zinc porphyrin nanocrystal and its preparation method and application. Background technique [0002] Photoacoustic imaging is a new non-radiation and non-invasive imaging examination method, which combines the advantages of high penetration depth, high sensitivity and high contrast of ultrasonography, and can provide high-resolution and high-contrast tissue imaging. Hotspots and new fields of tumor research in recent years. The photoacoustic signal comes from when the laser is irradiated in the biological tissue, the light absorption of the photoacoustic imaging molecules causes the tissue to undergo thermoelastic expansion, and an ultrasonic signal is generated. Endogenous chromophores and exogenous contrast agents are the main photoacoustic imaging molecules, especially the application of exogenous photoacoustic contrast agents has promoted the development of photoacoustic i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/22A61K9/107A61K9/19A61K41/00A61K47/24A61P35/00
CPCA61K9/107A61K9/19A61K41/0071A61K47/24A61K49/22A61K49/222A61P35/00
Inventor 李维平王晓君谭回彭耀南
Owner THE SECOND PEOPLES HOSPITAL OF SHENZHEN
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