Beauveria bassiana three-stage culture medium and preparation method thereof

Abstract

The invention discloses a beauveria bassiana three-stage culture medium and a preparation method thereof. The beauveria bassiana three-level culture medium comprises, by weight, the following raw materials: 20-30 parts of husk, 20-30 parts of wheat bran, 0.001-0.01 part of oxytetracycline and 30-50 parts of water. KH2PO4 may also be included. By adding oxytetracycline in the culture medium formulaand the used water, most microorganisms in the tap water are killed, the low-microorganism environment state of the long-term culture medium of the beauveria bassiana of 5-7 days is further ensured,and the beauveria bassiana grows into a dominant strain in an open environment. By adopting the culture medium to carry out three-stage culture, the produced beauveria bassiana high spore pollen can reach the highest value of 230 billion per gram.

Description

technical field [0001] The invention relates to the field of preparation of Beauveria bassiana, in particular to a three-stage culture medium for Beauveria bassiana and a preparation method thereof. Background technique [0002] Beauveria bassiana (Beauveria bassiana) is an ascomycete fungus, which can be industrially cultivated and produced, and the conidia produced by it can be processed into microbial pesticides. Beauveria bassiana high sporopollen is non-toxic, tasteless, non-environmental pollution, and has continuous infectivity to pests. Once the pests are infected, they can be continuously disseminated and spread; they can parasitize more than 700 species of insects in 149 families of 15 orders, and are relatively safe for humans, animals and the environment. Pests are generally not easy to develop drug resistance, and can be used with certain chemical pesticides (insecticides, acaricides, fungicides) at the same time. The most successful control of Beauveria bassia...

AI Technical Summary

Problems solved by technology

Beauveria bassiana spore powder currently produced in the market has a sporulation rate of about 100 billion / g, and the yield is low, and there are many miscellaneous bacteria. The choice of medium has a lot of influence on it