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Method for promoting rapid formation of hypha ball

A technology of mycelium balls and rotating speed, which is applied in the field of environmental microorganisms, can solve the problems of difficult to obtain quickly, affect the application of mycelium balls, and low ball formation rate, so as to shorten the time of ball formation, compact external structure, and high ball formation rate Effect

Active Publication Date: 2019-04-16
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The traditional method of cultivating mycelium balls is to use appropriate temperature, pH and rotation speed to allow them to form the required mycelium balls during the self-growth process of the fungus. Generally, it takes about 5 to 7 days, and the ball formation rate is low. It is difficult to obtain the purpose of quick acquisition, which affects the application of mycelium balls in real life

Method used

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  • Method for promoting rapid formation of hypha ball
  • Method for promoting rapid formation of hypha ball
  • Method for promoting rapid formation of hypha ball

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041](1) Prepare the bacterial suspension of Talaromyces flavus S1: take Talaromyces flavus S1 preserved on the slant medium, inoculate it in sterilized water with glass beads, place it on a shaker at 28±2°C for shaking culture, and perform hemocytosis Counting, the obtained concentration is 6.7×10 7 Individual / L of spore suspension of Talaromyces flavus S1.

[0042] (2) Cultivate Talaromyces flavus S1 mycelial balls: Take an appropriate amount of spore suspension and inoculate them into 12 PDB medium at a volume ratio of 10%, and divide them into four groups, namely blank group, control group 1, and control group 2 and test group 1. There are three shake flasks in each group as three parallels. The four groups were cultured in four shakers respectively. The rotation speed of the blank group was 0, the rotation speed of the control group 1 was 150 rpm, the rotation speed of the control group 2 was 200 rpm, and the rotation speed of the test group 1 was set at 200 rpm, and ...

Embodiment 2

[0050] (1) Get the mycelia balls prepared by each group in 10ml of Example 1, and add it to NO by a volume ratio of 10% 3 — In 100ml nitrate wastewater (Table 2) of N135.48mg / L, COD 1036.5mg / L, pH 7.9, observe the adsorption capacity of mycelium balls to nitrate wastewater. Measure COD and NO every 6 hours 3 - -N removal rate, the result is as figure 2 , as shown in 3.

[0051] Table 2

[0052]

[0053] figure 2 For the mycelium ball prepared in Example 1 to NO 3 - -N removal case. Mycelial balls with a rotational speed of 0 (blank group), the NO 3 - -N removal rate was 2.04%. When the rotating speed was 150rpm (control group 1), the mycelium NO 3 - -N removal rate was 8.15%. When the rotating speed was 200rpm (control group 2), the mycelium NO 3 - -N removal rate was 12.04%. However, during the cultivation process, the rotating speed of the shaking table was adjusted to 200rpm-150rpm-200rpm (test group 1) successively, and the hyphae balls had no effect ...

Embodiment 3

[0059] (1) Take 10 mL of mycelium balls prepared by each group in Example 1, add 10% volume ratio to 100 mL of ammonia nitrogen wastewater (Table 3), and observe the adsorption capacity of mycelium balls to nitrate wastewater. Measure COD and NH every 6 hours 4 + -N removal rate ( Figure 5 , 6).

[0060] table 3

[0061]

[0062] Such as Figure 5 As shown, when the rotating speed is 0 (blank group), the removal rate of ammonia nitrogen in the ammonia nitrogen wastewater by the mycelium ball formed by it is only 4.26%, which is negligible. When the rotating speed was 150rpm (control group 1), the ammonia nitrogen removal efficiency of the mycelial balls formed reached 12.47%; when the rotating speed was 200rpm (control group 2), the ammonia nitrogen removal rate of the mycelial balls formed was 17.58%. , Stronger adsorption capacity than mycelial balls formed at 150rpm. However, when the rotational speed was changed during the cultivation process, the mycelial balls ...

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Abstract

The invention discloses a method for promoting the rapid formation of a hypha ball and belongs to the field of environmental microorganisms. By controlling the rotating speed conditions in the hypha ball culture process in stages, compared with a prior method for culturing the hypha ball by controlling a constant rotating speed, the method not only has the advantages of simple operation, high hypha ball forming rate, high repeated utilization rate and the like, but also effectively shortens the hypha ball forming time, improves the yield of the hypha ball and enhances the adsorption capacity of the hypha ball. The invention not only provides a basis for the expanded culture of the talaromycesflavus S1 hyphae ball, but also provides a method for the application of the talaromycesflavus S1 hyphae ball in sewage treatment.

Description

technical field [0001] The invention relates to a method for promoting the rapid formation of mycelium balls, which belongs to the field of environmental microorganisms. Background technique [0002] Filamentous fungi widely exist in various items such as air and soil. They play an important role in agriculture, food and environmental protection. They can produce many metabolites with economic value, such as antibiotics and organic acids. Great industrial and social value. [0003] Mycelium balls are biological pellets formed spontaneously by filamentous fungi during fermentation. When the filamentous fungi are under the conditions of sufficient nutrients, appropriate shearing force, and dissolved oxygen, the germinated filamentous fungal spores form mycelia, and under the action of shearing, they twist around each other to form dense surfaces and loose interiors. Mycelial aggregates. Due to its large surface area and network structure, it is conducive to the mass transfe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C02F3/34C12R1/645C02F101/16
CPCC02F3/347C02F2101/16C02F2101/163C12N1/14
Inventor 缪恒锋周梦娟陆震明阮文权
Owner JIANGNAN UNIV
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