Method for efficiently recovering hydrogen from cellulose by synergistically utilizing thermophilic bacterial flora based on microbial electrolytic cell

A microbial electrolytic cell and cellulose technology, applied in the field of microbial electrolytic cell hydrogen production, can solve the problems of low rate and efficiency, increased complexity and cost, shorten the cycle of hydrogen production, promote rapid hydrolysis and fermentation, and avoid system pH drastic drop in effect

Active Publication Date: 2019-04-16
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, at room temperature, the rate and efficiency of MEC to produce hydrogen from ce...

Method used

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  • Method for efficiently recovering hydrogen from cellulose by synergistically utilizing thermophilic bacterial flora based on microbial electrolytic cell
  • Method for efficiently recovering hydrogen from cellulose by synergistically utilizing thermophilic bacterial flora based on microbial electrolytic cell
  • Method for efficiently recovering hydrogen from cellulose by synergistically utilizing thermophilic bacterial flora based on microbial electrolytic cell

Examples

Experimental program
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Effect test

Embodiment 1

[0030] 1) Weigh 10.0g of compost and place it in a 120mL serum bottle, weigh 0.25g of cellulose filter paper (cut the filter paper into 1cm*1cm pieces), then add 100mL of matrix culture solution into the serum bottle, and reserve 20mL of top Empty locations to collect gas. The matrix culture solution is prepared by mixing basic matrix, trace elements, vitamin elements, selenate and cysteine ​​hydrochloride solution, and each 1L of matrix culture solution contains 996mL of basic matrix, 1mL of trace element solution, vitamin solution (ATCC Vitamin) 1mL, selenate solution 1mL, cysteine ​​hydrochloride 1mL, wherein each 1L basic matrix contains the following ingredients: NaCl 1.0g, NH 4 Cl 10.0g, MgCl 2 ·6H 2 O 1.0g, CaCl 2H 2 O 5.0g; every 1L trace element solution contains the following ingredients: FeCl 2 4H 2 O 2.0g, H 3 BO 3 0.05g, ZnCl 2 0.05g, CuCl 2 2H 2 O 0.038g, MnCl 2 4H 2 O 0.05g, CoCl 2 ·6H 2 O 0.05g, NiCl 2H 2 O 0.092g, (NH 4 ) 2 Mo 7 o 24 4H 2...

Embodiment 2

[0039] 1) Weigh 10.0g of compost and place it in a 120mL serum bottle, weigh 0.25g of cellulose filter paper (cut the filter paper into 1cm*1cm pieces), then add 100mL of matrix culture solution into the serum bottle, and reserve 20mL of top Empty locations to collect gas. The preparation of the matrix culture solution is the same as the matrix culture solution in Step 1 in Example 1. After the matrix culture solution was prepared, use N 2 / CO 2 =80 / 20 mixed gas The serum bottle added with compost, filter paper and matrix was aerated for 30 minutes to remove dissolved oxygen in the solution while maintaining the initial pH of the matrix at 7. After the aeration, the serum bottle was sealed and placed in a shaker at 55°C for cultivation. During the cultivation, the gas volume was measured every 2 days with a glass syringe, and the gas composition was measured with a gas chromatograph. A round of culture ends when the cellulose is completely dissolved and no gas is produced ...

Embodiment 3

[0045] 1) Weigh 10.0g of compost and place it in a 120mL serum bottle, weigh 0.25g of cellulose filter paper (cut the filter paper into 1cm*1cm pieces), then add 100mL of matrix culture solution into the serum bottle, and reserve 20mL of top Empty locations to collect gas. The culture solution includes a basic matrix, trace elements, vitamin elements, selenate and cysteine ​​hydrochloride solution, and the preparation of the matrix culture solution is the same as the matrix culture solution in Step 1 in Example 1. After the matrix culture solution was prepared, use N 2 / CO 2 =80 / 20 mixed gas The serum bottle added with compost, filter paper and matrix was aerated for 30 minutes to remove dissolved oxygen in the solution while maintaining the initial pH of the matrix at 7. After the aeration, the serum bottle was sealed and placed in a shaker at 55°C for cultivation. During the cultivation, the gas volume was measured every 2 days with a glass syringe, and the gas compositio...

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Abstract

The invention discloses a method for efficiently recovering hydrogen from cellulose by synergistically utilizing a thermophilic bacterial flora based on a microbial electrolytic cell. The method comprises the following steps of (1) at the temperature of 55 DEG C, taking a compost as an inoculum, taking cellulose as an electron donor, and culturing a thermophilic cellulose degradation flora in a serum bottle; (2) constructing and starting a single-chamber MEC system, taking a compost as an inoculum, taking sodium acetate as an electron donor, and domesticating a thermophilic electrogenic bacterial flora on an MEC anode in a constant temperature incubator at the temperature of 55 DEG C; (3) adding enriched thermophilic cellulose degrading flora bacterial solution into the started single-chamber MEC, taking cellulose as an electron donor, operating the MEC based on the synergistic effect of the cellulose degrading flora and the electrogenic bacterial flora, and collecting hydrogen. The method utilizes thermophilic cellulose degrading bacteria to realize hydrogen production through hydrolysis and fermentation of cellulose and utilizes the thermophilic electrogenic bacterial flora to carry out in-situ utilization on cellulose fermentation end products (acetic acid) and generate hydrogen, so that chemical energy in the cellulose is efficiently converted to hydrogen energy.

Description

technical field [0001] The invention relates to a method for producing hydrogen by a microbial electrolytic cell, in particular to a method for recovering hydrogen from cellulose by utilizing the synergistic effect of thermophilic cellulose-degrading bacteria and thermophilic electrogenic bacteria. Background technique [0002] With the increasing energy crisis, the development of renewable energy has been highly valued by countries all over the world. At present, hydrogen energy is considered as an ideal renewable energy because of its high combustion calorific value, pollution-free combustion process, wide application range, abundant raw materials, and storage and transportation. At present, hydrogen production technologies mainly include electrocatalytic water splitting hydrogen production, dark fermentation hydrogen production, microbial electrolysis cell (MEC) hydrogen production and photocatalytic water splitting hydrogen production technology. Among them, the prospec...

Claims

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Application Information

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IPC IPC(8): C12P3/00
CPCC12P3/00Y02P20/129
Inventor 侯燕萍覃善铭涂玲丽闫一旻
Owner GUANGXI UNIV
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