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Ephedra homo polysaccharide ESP-B4 as well as preparation method and application thereof

A technology of ESP-B4 and Ephedra, applied in the field of Ephedra homogeneous polysaccharide ESP-B4 and its preparation, can solve the problems of side effects, lack of therapeutic drugs and the like

Active Publication Date: 2019-04-26
王秋红
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Simple anti-inflammatory and anti-pathogenic bacteria treatment is difficult to change the occurrence and development of lung injury, and there is a lack of effective therapeutic drugs in clinical practice. At present, only a large number of hormones can be used to prevent inflammation and reduce lung injury, which also brings great side effects during treatment.

Method used

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  • Ephedra homo polysaccharide ESP-B4 as well as preparation method and application thereof
  • Ephedra homo polysaccharide ESP-B4 as well as preparation method and application thereof
  • Ephedra homo polysaccharide ESP-B4 as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Example 1: Preparation method of Ephedra sinica homogeneous polysaccharide ESP-B4

[0054] 200kg of dried herbaceous stem meal of ephedra sinica, heated and refluxed with 95% industrial ethanol for 3 times, 3 hours each time, to remove alkaloids, pigments, polyphenols and oligosaccharides in ephedra, and then the dregs after extraction are treated with ephedra Extraction of polysaccharides. The medicinal residue was decocted with water for 3 times, each time for 3 hours, and filtered after cooling and combined the filtrate. The extracted dregs are naturally dried, the filtrate is recovered under reduced pressure, and lyophilized to obtain 14kg of total polysaccharides (PB) extracted from Ephedra sinica. After IRA-401 (5×50cm, id) + Amberlite FPC3500 (5×50cm, id) anion and cation series Resin column, first use distilled water as eluent, flow rate 10ml / min, phenol-sulfuric acid method detection, when sugar-free components flow out, change to 1M NaCl elution, until sugar-fre...

Embodiment 2

[0061] Example 2: An anti-acute lung injury and / or respiratory distress syndrome (ALI / ARDS) tablet

[0062] Take an appropriate amount of the homogeneous polysaccharide ESP-B4 prepared in Example 1, add diluents, disintegrants and other auxiliary materials, mix them, make granules, dry, tablet, coat or spray film to obtain.

Embodiment 3

[0063] Example 3: An oral liquid against acute lung injury and / or respiratory distress syndrome (ALI / ARDS)

[0064] Take an appropriate amount of the homogeneous polysaccharide ESP-B4 prepared in Example 1, add an appropriate amount of water to dissolve, mix well, add correctives and preservatives, mix well, dispense, sterilize, and get ready.

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Abstract

The invention relates to ephedra homo polysaccharide ESP-B4 as well as a preparation method and an application thereof. The ephedra homo polysaccharide ESP-B4 is a polysaccharide monomer prepared fromephedra, and is acidic heteropolysaccharide. An HPSEC-ELSD map shows a single symmetrical peak. The polysaccharide is prepared from xylose, arabinose, glucose, rhamnose, mannose, galactose, glucuronic acid and galacturonic acid in a molar ratio of 1.0:4.5:1.0:2.0:1.0:5.5:1.5:50 and has molecular weight of 2.37*107 Da, wherein the content of galacturonic acid is 75.2% or higher. The ephedra homogeneous polysaccharide ESP-B4 has significant infectious ALI (acute lung injury) resistance activity and can be used for treating severe infectious ALIcaused by SARS virus, influenza A virus and avian influenza virus as well as lung injury caused by common infections such as escherichia coli, pseudomonas aeruginosa, pneumococcus and influenza virus, has obvious treatment effect, is safe and has no side effect and is suitable for industrial production, and the preparation method is simple.

Description

Technical field [0001] The invention belongs to the field of medicine, and specifically relates to a homogeneous polysaccharide ESP-B4 of Ephedra and its preparation method and application. Background technique [0002] Acute lung injury (ALI) can worsen to respiratory distress syndrome (Acuterespiratory distress syndrome, ARDS), which manifests as rapid respiratory failure and severe arterial blood hypoxia that is not easy to be treated by oxygen infusion, leading to multiple organ failure, High fatality rate. It is a critical illness with high clinical morbidity and mortality. The case fatality rate of ALI / ARDS is as high as 45%-50%. If it is accompanied by sepsis, the case fatality rate is as high as 90%. The case fatality rate with multiple organ dysfunction will further increase. If there are more than 4 organ disorders, the case fatality rate will be 100 %. The clinical manifestations of major infectious diseases such as SARS, influenza A and avian influenza are acute lu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/715A61P11/00A61P31/04A61P31/14A61P31/16
CPCA61P11/00A61P31/04A61P31/14A61P31/16A61K31/715C08B37/0003C08B37/006A61K2236/331A61K2236/39A61K2236/53A61K36/17A61K9/0095A61K9/0019Y02A50/30A61K9/19A61K9/20A61K9/4808
Inventor 王秋红匡海学夏永刚杨炳友邢娜林晓婷
Owner 王秋红