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A talc-based Cordyceps javanica spore preparation
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A preparation and matrix technology, applied in the field of biological control, to achieve strong infection and insecticidal effects, low development and registration costs, and good environmental compatibility
Active Publication Date: 2021-03-12
SOUTH CHINA AGRI UNIV
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Problems solved by technology
However, it is still rare in the market to obtain a microbial pesticide that is highly efficient and has no pollution to the safe environment of humans and animals.
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Embodiment 1
[0029] Example 1 Isolation and Identification of Cordyceps Javanese Strain GZQ-1
[0030] 1. Source of materials
[0031] (1) Sample: Cordyceps javanica was preserved by the Biological Control EngineeringResearch Center of the Ministry of Education, South China Agricultural University. The preserved sample of this strain was purified by single cell separation.
[0032] (2) Potato Dextrose Agar Medium (PDA): 200g peeled potatoes, cut into small pieces, add 1L of water, boil for 20min, filter with gauze: add 20g of glucose while hot, 18-20g of agar, stir to dissolve and mix, set Fill it into an Erlenmeyer flask and sterilize it in an autoclave (121°C, 30min) for 30min.
[0033] (3) Aseptic operating conditions: All utensils and utensils must be sterilized in a pressure cooker (121°C, 30min), and operations such as inoculation are performed in an ultra-clean workbench.
[0034] (4) Culture conditions: culture in a 25°C light (12L:12D) incubator, transfer to a PDA slope after t...
Embodiment 2
[0058] The preparation of embodiment 2 talc base Cordyceps javanica spore preparation
[0059] 1. In the talc-based Cordyceps javanica spore preparation, the weight percentage ratio of each component is as follows:
[0061] 2. The preparation method of the preparation is:
[0062] According to the above ratio, mix honey, yeast and sterile water to form a medium, place the mycelia of Cordyceps javanica in the medium and shake the bacteria for 3 days, then put the obtained bacterial liquid, cellulose, and Tween-80 into talcum powder After being fully stirred by a stirrer and dried for 72 hours, a talc-based Cordyceps javanica spore preparation was made.
[0063] 3. Determination of spore germination rate of talc matrix Cordyceps javanica spore preparation
[0064] After storing the preparation prepared above for 2 and 15 days, fully mix the prep...
Embodiment 3
[0078] The preparation of embodiment 3 talc base Cordyceps javanica spore preparation
[0079] 1. In the talc-based Cordyceps Javanese spore preparation, the weight percentage ratio of each component is as follows: 0.4% of Cordyceps Javanese mycelium, 2.0% of honey, 0.4% of yeast, 45% of sterile water, 0.4% of cellulose, Tween-800.8 %, talcum powder 51%.
[0080] 2. The preparation method is the same as in Example 1. The results of the determination of the spore germination rate of the preparation show that the germination rate is high and the storage time is long.
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Abstract
The invention discloses a talc-based Cordyceps Javanese spore preparation, which is composed of Cordyceps Javanese ( Cordyceps javanica ), honey, yeast, sterile water, cellulose, Tween-80, talcum powder. The spores in the preparation can be stored for a longer period of time, and when used at the same time, it is conducive to the germination of the spores. It has a strong infestation and insecticidal effect on agricultural pests such as citrus psyllids. It is a broad-spectrum and efficient microbial insecticide. The natural environment has the characteristics of strong selectivity and has been obtained. The preparation has no environmental pollution, good compatibility with the environment, and is biologically safe to humans, animals, and natural enemies. It has selectivity for poisoning target pests and is not easy to produce drug resistance. Moreover, the production process is simple, convenient for large-scale production, and has a good application prospect in the biological control of agricultural pests.
Description
technical field [0001] The invention belongs to the technical field of biological control. More specifically, it relates to a talc-based Cordyceps javanica spore preparation. Background technique [0002] Insect pests such as Bemisia tabaci (Gennadius) and citrus psyllid (Diaphorinacitri Kuwayama) have caused serious losses to the agricultural fruit industry. Due to the long-term use of chemical pesticides, natural enemies of pests have disappeared, and resistance to conventional insecticides has increased. As well as the impact of chemical pesticides on humans and the ecological environment, it has led to the emergence of laws and regulations restricting the application of chemical pesticides in various countries around the world. These regulations not only make many chemical insecticides useless, but also greatly increase the development cost of new insecticides, thereby increasing interest in biological means of pest control. [0003] In the comprehensive prevention and...
Claims
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