Microporous RTM (regenerative tissue matrix) as well as preparation and application thereof

A technology for regenerating tissue and micropores, which is applied in medical science and prostheses, can solve the problems of large three-dimensional structure of collagen fibers, reduce the survival rate of dermal matrix transplantation, and affect the structure of dermal matrix proteins, etc., and achieve uniform micropores. distribution, improving the quality of wound healing, and increasing the survival rate

Active Publication Date: 2019-05-03
石卫华
View PDF11 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In the existing preparation methods and punching techniques of allogeneic dermis, in order to avoid the existence of immunogenic substances, most of them adopt relatively severe preparation conditions, which result in a large amount of degradation of the reticular fibers and elastic fibers of the dermal matrix, making the three-dimensional collagen fibers The structure is greatly broken, which reduces the survival rate of dermal matrix transplantation; in the dermal matrix perforation technology, rehydrated dermal matrix or wet dermal matrix is ​​often used for perforation, and most of the perforation processes are performed in acellular dermis. After the matrix is ​​prepared, the pore size is mostly controlled at 80-140um, but there are fewer cell attachment sites on the inner surface of the pore
Chinese patent CN105688286A discloses a laser microporous acellular dermal matrix and its preparation method. In the patent, a pulsed laser is used to perforate the dermal matrix. The raw material is prepared wet acellular dermal matrix, although its pore size distribution is uniform. , but with fewer cell attachment sites in its pore size
Chinese patent CN200510126108.6 discloses an acellular dermal matrix and its preparation method. In the patent, high-concentration sodium hydroxide is used to repeatedly wash the mammalian dermis to obtain the acellular dermal matrix. The severe alkali treatment conditions cause partial collagen denaturation and protein lysis. Original protein structure in the dermal matrix

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Microporous RTM (regenerative tissue matrix) as well as preparation and application thereof
  • Microporous RTM (regenerative tissue matrix) as well as preparation and application thereof
  • Microporous RTM (regenerative tissue matrix) as well as preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] The preparation method of the microporous regenerative tissue matrix of the present invention comprises the following steps:

[0047] 1. Washing: Take the allogeneic skin out of the storage protection solution, scrape off the subcutaneous tissue, put it into physiological saline and shake and wash it, the ratio of the area of ​​the skin to the material-liquid of the physiological saline (cm 2 :mL) is 1:1, the total volume of feed liquid does not exceed 50% of the capacity of the shaking container, the shaking frequency is 30rpm, the shaking cleaning time is 1.5h, and the normal saline is replaced once every shaking for 0.5h, and a total of shaking and washing is done 3 times.

[0048] 2. Remove the epidermis: soak the allogeneic skin that has been shaken and washed in a 1mol / L NaCl solution, the ratio of solid to liquid (cm 2 : mL) at a ratio of 1:1, soak for 24 hours at 28°C. After soaking, the allogeneic skin is taken out, and the epidermis is torn off. The skin fro...

Embodiment 2

[0059] The preparation method of the microporous regenerative tissue matrix of the present embodiment comprises the following steps:

[0060] Step 1-5 is identical with embodiment 1;

[0061] 6. Rehydration treatment: Gradient-decreasing rehydration method is adopted, that is, the freeze-dried microporous allograft dermis is placed in 400 mmol / L trehalose low-concentration saline solution, and the solid-liquid ratio (cm 2 :ml) is 1:1, shake and soak for 15min; then take it out and add it to 200mmol / L trehalose low-concentration brine 2 :ml) is: 1:1, oscillating and soaking for 15min; 2 :ml) ratio: 1:1, shaking and soaking for 15 minutes, the microporous allogeneic dermis can be obtained.

[0062] 7. Alkaline expansion decellularization treatment: add microporous allogeneic dermis to 0.2mol / L Na 2 CO 3 Soaking treatment in medium, solid-liquid ratio (cm 2 :ml) 1:1, the total volume of the feed liquid does not exceed 50% of the oscillator capacity, the oscillation frequency...

experiment example 1

[0077] Experimental example 1: Performance testing of regenerative tissue matrix

[0078] Biological properties such as mechanical properties of the regenerated tissue matrix prepared in Examples 1 and 2 and Comparative Example 1 were tested, and the performance indicators included tensile strength, suture strength, and tensile elongation. The specific test results are shown in Table 1. The results show that the products of Examples 1 and 2 have a better fit to the skin, and have good toughness and elasticity, and can meet the requirements for the suture strength of the patch after removal of the giant mole.

[0079] Table 1 Test results of microporous regenerative tissue matrix

[0080] project

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
pore sizeaaaaaaaaaa
Login to view more

Abstract

The invention provides a microporous RTM (regenerative tissue matrix) as well as preparation and application thereof. The microporous RTM is a flaky material of a porous structure, micropores are uniformly distributed in the material, the pore diameter of the micropores is 0.15-0.20 mm, the distance between micropores is 0.8-1.2 mm, and collagen fiber extending into the pores is arranged on the inner surfaces of the micropores. The microporouse RTM provided by the invention removes cells completely and has no immunogenicity or cytotoxicity factors, collagen fiber, reticular fiber, elastic fiber of the RTM and a three-dimensional structure consisting of the fibers are slightly damaged, the RTM is better attached to wounds, multiple collagen fibers extending into the pores are arranged on the inner surfaces of the mciropores, and more immigrated cell attachment sites can be provided.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and in particular relates to a microporous regeneration tissue matrix, its preparation method and application. Background technique [0002] Regenerative Tissue Matrix (RTM) is a series of physical and chemical methods to remove the cellular components in the epidermis and dermis of human skin, remove the immunogenicity caused by foreign bodies, and retain the collagen fibers and reticular fibers of the dermis , Elastic fibers and their three-dimensional structure. In 1995, American Life Cell Company first reported the preparation method of acellular dermal matrix, and at the same time carried out its clinical application research in the replacement repair of dermal defects caused by burns and trauma. Subsequent studies have found that acellular dermal matrix, in addition to being used for wound repair such as burns and trauma, can also be used as a substitute for mucosa and soft tissues, and...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/56A61L27/60
Inventor 莫丽影孙善凤石卫华王姣王正元
Owner 石卫华
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap