Microporous RTM (regenerative tissue matrix) as well as preparation and application thereof

Abstract

The invention provides a microporous RTM (regenerative tissue matrix) as well as preparation and application thereof. The microporous RTM is a flaky material of a porous structure, micropores are uniformly distributed in the material, the pore diameter of the micropores is 0.15-0.20 mm, the distance between micropores is 0.8-1.2 mm, and collagen fiber extending into the pores is arranged on the inner surfaces of the micropores. The microporouse RTM provided by the invention removes cells completely and has no immunogenicity or cytotoxicity factors, collagen fiber, reticular fiber, elastic fiber of the RTM and a three-dimensional structure consisting of the fibers are slightly damaged, the RTM is better attached to wounds, multiple collagen fibers extending into the pores are arranged on the inner surfaces of the mciropores, and more immigrated cell attachment sites can be provided.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and in particular relates to a microporous regeneration tissue matrix, its preparation method and application. Background technique [0002] Regenerative Tissue Matrix (RTM) is a series of physical and chemical methods to remove the cellular components in the epidermis and dermis of human skin, remove the immunogenicity caused by foreign bodies, and retain the collagen fibers and reticular fibers of the dermis , Elastic fibers and their three-dimensional structure. In 1995, American Life Cell Company first reported the preparation method of acellular dermal matrix, and at the same time carried out its clinical application research in the replacement repair of dermal defects caused by burns and trauma. Subsequent studies have found that acellular dermal matrix, in addition to being used for wound repair such as burns and trauma, can also be used as a substitute for mucosa and soft tissues, and...

AI Technical Summary

Problems solved by technology

[0007] In the existing preparation methods and punching techniques of allogeneic dermis, in order to avoid the existence of immunogenic substances, most of them adopt relatively severe preparation conditions, which result in a large amount of degradation of the reticular fibers and elastic fibers of the dermal matrix, making the three-dimensional collagen fibers The structure is greatly broken, which reduces the survival rate of dermal matrix transplantation; in the dermal matrix perforation technology, rehydrated dermal matrix or wet dermal matrix is ​​often used for perforation, and most of the perforation processes are performed in acellular dermis. After the matrix is ​​prepared, the pore size is mostly controlled at 80-140um, but there are fewer cell attachment sites on the inner surface of the pore

Chinese patent CN105688286A discloses a laser microporous acellular dermal matrix and its preparation method. In the patent, a pulsed laser is used to perforate the dermal matrix. The raw material is prepared wet acellular dermal matrix, although its pore size distribution is uniform. , but with fewer cell attachment sites in its pore size

Chinese patent CN200510126108.6 discloses an acellular dermal matrix and its preparation method. In the patent, high-concentration sodium hydroxide is used to repeatedly wash the mammalian dermis to obtain the acellular dermal matrix. The severe alkali treatment conditions cause partial collagen denaturation and protein lysis. Original protein structure in the dermal matrix

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