Preparation method and application of monodisperse superparamagnetic nano magnetic bead

A magnetic nanometer and superparamagnetic technology, which is applied in the manufacture of magnetic materials, magnetic objects, inductors/transformers/magnets, etc., can solve problems such as poor suspension stability, reduced magnetic responsiveness, and reduced magnetic content, and achieve low preparation costs. Simple preparation method, good adsorption effect

Inactive Publication Date: 2019-05-07
广州奇辉生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In order to achieve high magnetic responsivity, magnetic beads with large particle size are usually selected, and the large particle size correspondingly leads to poor suspension stability and low adsorption capacity for target products
By increasing the polymer coating layer to improve its adsorption capacity, the increase in the content of non-magnetic substances will inevitably lead to a decrease in the magnetic content, that is, a decrease in magnetic responsiveness.
[0007] In addition, the surface modification of magnetic beads currently on the market is usually not ideal, the properties of the modified layer on the surface are not stable enough, and the properties of long-term storage will change slowly. In addition, there are many requirements for the environmental medium used.
Moreover, the number of functional groups on the surface of the magnetic beads is not rich enough, and the reactivity is not high, that is, the adsorption capacity of the magnetic beads to the target product is low

Method used

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  • Preparation method and application of monodisperse superparamagnetic nano magnetic bead
  • Preparation method and application of monodisperse superparamagnetic nano magnetic bead
  • Preparation method and application of monodisperse superparamagnetic nano magnetic bead

Examples

Experimental program
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Effect test

Embodiment 1

[0035] Embodiment 1 The preparation method of the monodisperse superparamagnetic nano-magnetic beads with high adsorption performance of the present invention

[0036] (1) Preparation of monodisperse superparamagnetic nano-magnetic beads

[0037] The invention adopts the improved solvothermal method, innovatively adds PEG in the reaction system to improve the dispersibility of the product, thereby synthesizing monodisperse nano-Fe 3 o 4 Particles, good dispersion performance, uniform particle size. The specific preparation method is as follows: 15mmolFeCl 3Dissolve in 300ml of ethylene glycol, then add 3.6g of NaAc and 10g of PEG 2000, shake and mix until the solid is completely dissolved, transfer the reaction liquid to a reaction kettle, and react at 200°C for 8 hours. After the reaction is completed, use a magnetic frame to magnetically separate and recover monodisperse nano-Fe 3 o 4 Particles were washed with water and ethanol several times to remove residual reaction...

Embodiment 2

[0051] Example 2: Suspension stability and magnetic responsiveness test of magnetic beads

[0052] Use TE solution to prepare the magnetic beads (number PC) of the present invention to a concentration of 10mg / ml, and after mixing thoroughly: ①Stand at room temperature, and record the time from the homogeneous system to the complete settlement of the magnetic beads. ② After fully mixing, place on the magnetic stand, and record the time required for the magnetic beads to be completely adsorbed to the side of the magnet and the solution to be completely clarified.

[0053] PC is the magnetic bead finally obtained through surface hydroxylation, amino and carboxylation modification treatment in the present invention, A is the magnetic bead obtained only through surface hydroxylation treatment, and B is the magnetic bead obtained through surface hydroxylation and amino modification treatment , C is carboxyl magnetic beads purchased from Beckman Company of the United States.

[0054...

Embodiment 3

[0056] Embodiment 3: DNA recovery rate test-1

[0057] Disperse the magnetic beads in the PEG solution at a concentration of 1 mg / ml, use Marker (100bp-III DNA Ladder) as a test sample, and dilute its concentration to 10 ng / μl.

[0058] Take 50 μl of the diluted Marker (10ng / μl) in an EP tube, add 90 μl of magnetic beads (1mg / ml), and vortex for 5 minutes.

[0059] After brief centrifugation, place the EP tube on the magnetic stand. After the solution is clarified, discard the supernatant and wash the magnetic beads twice with 80% ethanol.

[0060] After drying the ethanol, add 50 μl TE solution, vortex for 3 minutes, place the centrifuge tube on the magnetic stand, and after the solution is completely clear, transfer the supernatant to a new EP tube to obtain the recovered product.

[0061] The concentration of the recovered product was measured, and then the recovery rate was calculated. The results are as follows (PC represents the magnetic beads of the present invention)...

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Abstract

The invention belongs to the technical field of magnetic material preparation, and particularly relates to a monodisperse superparamagnetic nano magnetic bead with a modified surface and a high adsorption performance and a preparation method thereof and application thereof. The method comprises the steps of: (1) preparation of monodisperse superparamagnetic nano Fe3O4 particles; (2) surface hydroxylation modification of Fe3O4 particles: performing two-layer coating for the Fe3O4 particles to form a mesoporous Fe3O4@SiO2 core-shell structure; (3) performing amination modification of the Fe3O4@SiO2 core-shell structure; and (4) performing surface carboxylation modification of the product of step (3). The preparation method is simple, efficient and low in preparation cost, is specially suitable for separation and purification of the DNA / RNA and protein in the biological fluid or other reaction liquids, can be suitable for automation, and can be suitable for clinical biochemistry fast automatic detection based on chemiluminiscence and reflective substance labeling.

Description

technical field [0001] The invention belongs to the technical field of magnetic material preparation, and in particular relates to a surface-modified monodisperse superparamagnetic nano-magnetic bead with high adsorption performance, a preparation method thereof, and its application in nucleic acid extraction and purification in the fields of medicine and biology. Background technique [0002] Magnetic beads are a new multifunctional material developed in recent years and widely used in the field of biomedicine. The structure of magnetic beads usually consists of a magnetic core and a polymer shell wrapped around the core. Due to the response of the magnetic core to the external magnetic field, it can be quickly aggregated under the action of a magnetic field, so that it can be quickly and completely separated in a dispersed solution. After canceling the magnetic field, it can quickly disperse again. In general, functional nano-magnetic beads need to meet several condition...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): H01F41/00H01F1/00
Inventor 朱奇罗李江廖传荣
Owner 广州奇辉生物科技有限公司
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