Method for screening hypoglycemic active components of cornus officinalis

Abstract

The invention relates to a method for screening hypoglycemic active components of cornus officinalis. The method comprises the following steps of: establishing a cornus officinalis aqueous extract component analysis method by using a chromatography-mass spectrometry technology, and identifying active components in cornus officinalis through data analysis; carrying out similarity comparison on chemical structures of the active components and a DrugBank database, and setting a similarity scoring card value to be 0.8 so as to obtain a component target; retrieving a disease / syndrome target database to obtain candidate disease targets of diabetes; carrying out related hypoglycemic effect target prediction on the chemical components of the cornus officinalis by adoption of a network pharmacologic technology, constructing a cornus officinalis component-hypoglycemic target-role access network, and predicting potential hypoglycemic active components and a molecular mechanism; and carrying out activity evaluation and experimental verification on the potential active components through cell experiment so as to determine real and effective hypoglycemic active components in the cornus officinalis. The method is capable of improving the cornus officinalis active component screening efficiency and reducing the drug active component screening cost.

Description

technical field [0001] The invention belongs to the research field of active ingredients of traditional Chinese medicines, and in particular relates to a method for screening active ingredients of cornus officinalis for hypoglycemia. Background technique [0002] The number of diabetic patients in my country accounts for about one-third of the total number of diabetic patients in the world. Diabetes and its complications have become the third biggest killer after cardiovascular and cerebrovascular diseases and cancer that threaten human life and health. Due to its complicated pathogenesis, it is still unknown. Find a cure for diabetes and its complications. Traditional Chinese medicine has a long history of thousands of years in the treatment of diabetes in my country. It has a certain basis for clinical application. It has the advantages of rich resources, convenient materials, and low toxicity. Under the guidance of modern medical theory, it has been confirmed that Chinese ...

AI Technical Summary

Problems solved by technology

At present, the research on the improvement of type 2 diabetes by Cornus officinalis mainly focuses on the total components, and the mechanism of action of the active ingredients of Cornus officinalis focuses on the regulation of glucose and lipid metabolism and the mechanism of action of insulin signaling pathways. Due to the diversity and complexity of the components contained in traditional Chinese medicine , there is a lack of systematic research and elaboration on its hypoglycemic active ingredients and their mechanism of action

[0005] At present, the research on the chemical constituents of Cornus officinalis is mainly based on the traditional separation and purification, and then the identification of its structure by spectrum technology. The analysis mode is cumbersome, and there are problems such as repeated separation of known constituents and difficulties in obtaining pure products of new constituents.

Due to the diversity and complexity of the ingredients contained in Cornus officinalis, its molecular mechanism for treating diabetes has not been fully elucidated. Each active ingredient has a different therapeutic mechanism for diseases. The interaction between molecular network construction and the acquisition of drug-target interaction has a certain effect on drug screening, but the molecular mechanism of diabetes is complex and has not been fully elucidated. Even if the drug-target network is fully obtained, it is still only The conventional hypoglycemic active ingredients of Cornus officinalis can be obtained, but the coordination relationship between these active ingredients cannot be known, so as to screen and obtain the most effective combination of hypoglycemic active ingredients on Cornus officinalis

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