Composite microbial agent for cassava residue matrix fermentation and preparation method and application thereof

A technology of composite microbial agent and cassava residue, which is applied in the field of composite microbial agent for cassava residue matrix fermentation and its preparation, can solve the problems of poor adaptability of cassava residue fermentation, achieve important scientific significance and application value, reduce toxic effects, The effect of increasing the fermentation temperature

Inactive Publication Date: 2019-05-14
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many compound microbial fermentation agents in the ma

Method used

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  • Composite microbial agent for cassava residue matrix fermentation and preparation method and application thereof
  • Composite microbial agent for cassava residue matrix fermentation and preparation method and application thereof
  • Composite microbial agent for cassava residue matrix fermentation and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1: Preparation method of microbial agent

[0033] Medium: (1) Meat extract peptone liquid medium: Beef extract 5.0g, peptone 10.0g, NaCl 5.0g, distilled water 1000ml, pH 7.4~7.6; (2) Gao's liquid medium: KNO 3 1g / L, soluble starch 20g / L, K 2 HPO 4 0.5g / L, MgSO 4 .7H 2 O 0.5g / L, NaCl 0.5g / L, FeSO 4 0.01g / L, 1000ml distilled water, pH 7.4-7.6; (3) Potato glucose liquid medium: potato 200g / L, glucose 20g / L, agar powder 18g / L, tap water 1000mL.

[0034] Step 1: Inoculate Bacillus highlandus and Bacillus licheniformis in two bottles of beef extract peptone liquid medium respectively, and expand and cultivate for 7 days in a shaker at 28°C and 270 revolutions.

[0035] The actinomycete microorganism Streptomyces erythropolis was amplified and cultured using Gao's No. 1 liquid medium, and cultured in a shaker at 28°C and 270 revolutions for 8 days.

[0036] The fungal microorganism Trichoderma viride was amplified and cultured in potato dextrose liquid medium and cultured in ...

Embodiment 2

[0040] The composite microbial inoculum prepared according to the method of Example 1, wherein the fermentation broth of Bacillus highlands (isolated in the laboratory) and the fermentation broth of Bacillus licheniformis (isolated in the laboratory) are composed of equal volumes of the bacterial inoculum and Streptomyces griseus (experimental) Room separation) bacterial liquid and Trichoderma viride (laboratory separation) bacterial liquid are mixed according to the volume ratio of 1:1:1 to form a composite microbial agent.

Embodiment 3

[0042] The composite microbial inoculum prepared according to the method of Example 1, wherein the bacteria-like liquid composed of the fermentation broth of Bacillus highlands (CGMCC1.8639) and the fermentation broth of Bacillus licheniformis (BNCC165853) and the bacteria of Streptomyces griseus (BNCC156263) Liquid and Trichoderma viride (GIM3.141) bacterial liquid are mixed according to the volume ratio of 1:2:1 to form a composite microbial agent.

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Abstract

The invention discloses a composite microbial agent for cassava residue matrix fermentation and a preparation method and application thereof. The composite microbial agent for cassava residue matrix fermentation comprises bacillus altitudinis, bacillus licheniformis, streptomyces griseorubens and trichoderma viride. The composite microbial agent for cassava residue matrix fermentation can accelerate temperature increase in the early stage of cassava residue fermentation, increase the fermentation temperature and prolong the time of the high-temperature period. The decomposition degree of a fermented product is reduced, the toxic effect on plants is reduced, and the fermentation quality is improved. The method has important scientific significance and application value on cassava residue matrix utilization.

Description

Technical field [0001] The invention belongs to the technical field of substrate microbial fermentation, and relates to a composite microbial inoculum used for cassava residue substrate fermentation and a preparation method and application thereof. Background technique [0002] Cassava is an important economic crop in southern my country. The cassava residue is the organic solid waste left after the alcohol in the cassava processing industry. It is mainly composed of cellulose, hemicellulose and lignin, and is rich in various trace elements beneficial to animals and plants. . At present, fresh cassava residue is difficult to use due to its high humidity. Except for a small part of it is used for animal feed, most of it is directly discharged into the environment without treatment, which not only causes waste of resources, but also affects the ecology. The environmental threat is greater. Therefore, the resource reuse of cassava residue has become an urgent problem for solid wast...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N1/20A01G24/20A01G24/27C12R1/07C12R1/10C12R1/465C12R1/885
Inventor 束胜王战郭世荣孙锦王玉胡金成杨雅兰王文晓张晓琳
Owner NANJING AGRICULTURAL UNIVERSITY
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