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Microecological preparation matched with glucose oxidase as well as preparation method and application of microecological preparation

A technology of glucose oxidase and microecological preparations, which is applied in the prevention and control of piglet diarrhea. In the field of microecological preparations, it can solve problems such as damage to the digestive tract of piglets, ecological environment and food safety hazards, and environmental pollution.

Inactive Publication Date: 2019-05-28
JINANBESTZYME BIO ENG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the main method of production is to add high doses of zinc oxide to the feed to prevent the impact of weaning stress on piglets. Although excessive zinc oxide has a good astringent effect on diarrhea, long-term addition will also cause damage to the digestive tract of piglets , cause negative impact on piglets, and also cause harm to the ecological environment and food safety
In addition, a large amount of unused zinc oxide is discharged into the environment with feces, causing environmental pollution
[0003] At present, the production is mainly to do a good job in health care before and after weaning, increase the palatability of rations, add functional feed additives, improve feeding management and feeding environment and other factors to alleviate the diarrhea caused by weaning stress. Products with the function of replacing zinc oxide on the market are also available. Mainly focused on different forms of zinc products, ultimately unable to break away from the level of metal pollution

Method used

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  • Microecological preparation matched with glucose oxidase as well as preparation method and application of microecological preparation
  • Microecological preparation matched with glucose oxidase as well as preparation method and application of microecological preparation
  • Microecological preparation matched with glucose oxidase as well as preparation method and application of microecological preparation

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The compatibility of embodiment 1 microecology and glucose oxidase

[0040] 1.1 Materials: Glucose oxidase (GOD) is selected from commercial feed glucose oxidase, and the microorganisms to be tested are selected from Bacillus licheniformis, Bacillus subtilis, Clostridium butyricum, Enterococcus faecium and Enterococcus faecalis.

[0041] The formula of microbial culture medium is as follows:

[0042] Bacillus licheniformis, Bacillus subtilis: LB+1% glucose-peptone 1%, yeast powder 0.5%, NaCl1%, glucose 1%;

[0043] Enterococcus faecium, Enterococcus faecalis: MRS broth medium;

[0044] Clostridium butyricum: tryptone 1.8%, yeast powder 0.5%, sodium metabisulfite 0.1%, ferric ammonium citrate 0.1%;

[0045] The above-mentioned culture medium was sterilized at 121°C for 30 minutes and used for later use.

[0046] 1.1.4 Physiological saline

[0047] Take 0.9g of NaCl, add 100mL of distilled water, sterilize by high-pressure steam at 121°C for 30min, and then cool it do...

Embodiment 2

[0066] Effect of embodiment 2 probiotics on production performance of weaned piglets

[0067] 2.1 Select the microorganisms (Clostridium butyricum, Enterococcus faecium and Enterococcus faecalis) that can be compatible with glucose oxidase in Example 1 and combine them with glucose oxidase to prepare feed additive HT01-04. The formula of each feed additive is shown in Table 3 shown.

[0068] Table 3 Contents of each component of feed additive HT01-04 ( / g feed additive)

[0069]

[0070] 2.2 Experimental design

[0071] 28-day-old weaned piglets with the same genetic background, the same batch, and normal development were selected and randomly divided into 7 groups of experiments I, II, III, IV, V, VI, and VII, with 3 replicates in each group and 10 piglets in each replicate , There was no significant difference in the initial body weight of weaned piglets among the treatment groups (P>0.05), the pre-feeding period was 3 days, and the test period was 30 days. The specific...

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Abstract

The patent application discloses a method for screening probiotics which can be matched with glucose oxidase. The screened probiotics can be prepared into a microecological preparation; the microecological preparation can be compounded with the glucose oxidase to obtain a feed additive. When the feed additive is used for feeding piglets, the diarrhea rate of weaned piglets can be reduced and thegrowth of the piglets also can be promoted. The feed additive as an environment-friendly green additive can be used for replacing zinc oxide so as to reduce the pollution to the environment.

Description

Technical field: [0001] The invention relates to the field of feed additives, in particular to a microecological preparation compatible with glucose oxidase and its application in preventing and controlling piglet diarrhea. Background technique: [0002] With the advancement of large-scale and intensive pig raising models, piglet diarrhea has become a decisive factor for the economic benefits of the farming industry, and it is also a bottleneck restricting the development of the pig raising industry. Piglet diarrhea is a common multiple disease, which can lead to growth stagnation, even growth regression, and severe cases cause death. There are many factors that cause piglet diarrhea, such as pathological factors of pathogenic microorganism infection, nutritional factors of intake of high-protein feed, weaning stress, feeding management, etc. Among them, weaning stress is a situation that piglets have to face in breeding, and it is the cause of piglet diarrhea. The top prio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K20/189A23K10/18A23K50/30A23K50/60
CPCA23K10/18A23K20/163A23K20/189A23K50/30A23K50/60C12Q1/04
Inventor 刘延杰赵素珍陈宁波陈锋亮宋迪
Owner JINANBESTZYME BIO ENG CO LTD