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A kind of flavonoid total glycosides of cloth dhay leaves and its preparation method and application

A technology of flavonoids from slag leaves and flavonoids from slag leaves, which is applied in the field of total glycosides of slag leaf flavonoids and its preparation, can solve problems such as undiscovered applications, achieve no toxic and side effects, improve liver tissue fatty degeneration, and enhance liver glucose and lipid metabolism The effect of toughness

Active Publication Date: 2021-06-18
瑞康东方医学研究(北京)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After searching, it has not been found about the application of total flavonoid glycosides of Buzaye in the preparation of NAFLD drugs and functional dietary supplements for enhancing the toughness of liver glucose and lipid metabolism

Method used

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  • A kind of flavonoid total glycosides of cloth dhay leaves and its preparation method and application
  • A kind of flavonoid total glycosides of cloth dhay leaves and its preparation method and application
  • A kind of flavonoid total glycosides of cloth dhay leaves and its preparation method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0043] like figure 1 Shown: a kind of preparation method of flavonoid total glycosides of cloth dregs leaf, comprises the following steps:

[0044] Take 4 kg of cloth residue leaves, add 50L of deionized water, heat and reflux for extraction twice, 2 hours each time; the extract is concentrated to 8 L under reduced pressure, centrifuged, and the supernatant is absorbed on a D101 or AB-8 macroporous resin column , followed by sequential elution with deionized water, 10%, 70%, and 95% ethanol, and concentrating the 70% ethanol eluent under reduced pressure to obtain the total flavonoids of cloth residue leaves, which were dissolved in an appropriate amount of methanol; and then separated by preparative chromatography (C18 Packing) water and methanol gradient elution, 280 nm wavelength detection, collect the eluate containing flavonoid glycosides, concentrate and spray dry to obtain total flavonoid glycosides of Buza leaves (MpTFG), and use HPLC for qualitative and quantitative ...

Embodiment 2

[0050] Example 2: Prophylactic administration of total flavonoid glucosides (MpTFG) improves liver steatosis and inflammation in diet-induced obese rats

[0051] Experimental Drugs and Reagents

[0052] Total cholesterol (TC), triglyceride (TG), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) kits were purchased from Shanghai Rongsheng Biopharmaceutical Co., Ltd. Rat tumor necrosis factor α (TNF-α), rat interleukin 1β (IL-1β) and rat interleukin 6 (IL-6) ELISA kits were provided by Wuhan Huamei Bioengineering Co., Ltd.

[0053] experimental animals

[0054] Twenty-four healthy male Spraque-Dawley rats, 5 weeks old, weighing 130-150 g, SPF grade, were purchased from Guangdong Experimental Animal Center, license number: SCXK (Guangdong) 2013-0002, certificate number: No.44007200052450 . The animals were kept in the SPF grade animal room of the Experimental Animal Center of Guangdong Pharmaceutical University. Animal breeding temperature: 20-25 ℃, humidity...

Embodiment 3

[0063] Example 3: Treatment of hepatic steatosis and inflammation in diet-induced obese rats with total flavonoid glycosides of Buza leaves (MpTFG)

[0064] Experimental Drugs and Reagents

[0065] Total cholesterol (TC), triglyceride (TG), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) kits are products of Shanghai Rongsheng Biopharmaceutical Co., Ltd. Rat tumor necrosis factor α (TNF-α), rat interleukin 1β (IL-1β) and rat interleukin 6 (IL-6) ELISA kits were provided by Wuhan Huamei Bioengineering Co., Ltd.

[0066] experimental animals

[0067] Twenty-four healthy male Spraque-Dawley rats, 5 weeks old, weighing 130-150 g, SPF grade, were purchased from Guangdong Experimental Animal Center, license number: SCXK (Guangdong) 2013-0002, certificate number: No.44007200052450 . The animals were kept in the SPF grade animal room of the Experimental Animal Center of Guangdong Pharmaceutical University. Animal breeding temperature: 20-25 ℃, humidity: 40%-70%...

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Abstract

The invention discloses total flavonoid glycosides of cloth dhay leaves and its preparation method and application. Flavonoid glycosides account for more than 50% of the total flavonoid glycosides of cloth dregs leaves, and the flavonoid glycosides include flavone carbon glycosides and flavone oxyglycosides , wherein, flavonoid carbon glycosides account for 30-50% of the mass of the total flavonoid glycosides of the cloth dha leaf, and flavonoid oxyglycosides account for 10-20% of the mass of the total flavonoid glycosides of the cloth dha leaf. The total flavonoid glycosides of Buza leaves of the present invention have been verified by experiments to effectively improve the fatty degeneration of rat liver tissue caused by HFHF diet, and can significantly reduce the metabolic inflammation of model rats, and the obtained total flavonoid glycosides are derived from medicinal and edible homologous plants , is safe and reliable, has no toxic and side effects, and can be used as an active ingredient in the preparation of drugs for the prevention and treatment of diet-induced NAFLD or metabolic inflammation, as well as functional dietary supplements for enhancing the toughness of glucose and lipid metabolism in the liver.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicines and natural medicines, and in particular relates to total flavonoid glycosides of Buza leaves and its preparation method and application. Background technique [0002] With the increasing normalization of high-fat and high-sugar diet, the incidence of non-alcoholic fatty liver disease (NAFLD) induced by it is increasing year by year, and there is a trend of younger age. NAFLD has become one of the common chronic liver diseases worldwide. NAFLD generally refers to hepatic steatosis caused by excessive fat deposition in liver cells, which is accompanied by liver cell damage, liver inflammation and fibrosis in severe cases. Due to the complex pathogenesis of NAFLD, there are no specific and efficient treatment drugs and programs, and the common practice is to control diet and drug intervention. Since NAFLD is often accompanied by fat deposition in the liver, lipid metabolism d...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/185A61P3/06A61P1/16A61P31/12A23L33/105
Inventor 李坤平郭姣
Owner 瑞康东方医学研究(北京)有限公司
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