Aerobic denitrification bacterium SLY2-21 applied to denitrifying sewage and application thereof
A technology of aerobic denitrification bacteria and aerobic denitrification, applied in aerobic process treatment, biological water/sewage treatment, microbial-based methods, etc., can solve the problems of limited practical application research and single type of bacteria discovery, To achieve the effect of broad application prospects
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0031] The specific steps for the screening of aerobic denitrifying bacteria are as follows:
[0032] 1. Acclimatization and enrichment: Inoculate 10mL of fully mixed fresh landfill leachate into a 250mL Erlenmeyer flask with 100mL LB medium, place on a shaker at 30°C, 150rpm, and cultivate for 48h, and take samples every 12h to qualitatively detect N0 in the medium 3 - -N and NO 2 - -N content. Take 10 mL of the enriched culture medium and transfer it to fresh LB liquid medium, transfer it 5 times, and enrich for 10 days in total.
[0033] Qualitatively detect N0 in the medium with diphenylamine reagent and Gliese reagent at intervals of 12 h 3 - -N and NO 2 - -N content. Use a sterilized dropper to draw 3 drops of culture solution into a sterilized test tube, and then add 3 drops of Griess reagent to the test tube. If the culture solution turns pink or brown, it proves the presence of nitrite. Nitrate reduction positive, the bacteria may be aerobic denitrification b...
Embodiment 2
[0047] Molecular biological identification of aerobic denitrifying bacteria SLY2-21:
[0048] After the above screening and separation, an aerobic denitrification strain SLY2-21 with good denitrification effect was obtained. The strain was Gram-negative, milky white, regular in shape, smooth and moist in surface, and easy to stir up by SEM. The scan found that the SLY2-21 bacteria were rod-shaped, with a size of 1-2um×0.3-0.5um (see figure 1 ).
[0049] 1. Bacterial genomic DNA extraction
[0050] Insert the target strain into LB medium, after culturing overnight, take 1 mL and centrifuge to obtain the bacteria, then use the genome kit to extract the bacterial genome, PCR amplification, agarose gel electrophoresis (1%) verification, and ultraviolet analyzer to check the electrophoresis results .
[0051] 2. PCR amplification of 16SrDNA fragments
[0052] Use universal primers (27f: 5'-AGAGTTTGATCCTGGCTCAG-3', 1492r: 5'-GGTTACCTTGTTACGACTT-3') to carry out PCR reaction, the...
Embodiment 3
[0059] Growth and denitrification characteristics of SLY2-21 strain:
[0060] 1. Inoculate the SLY2-21 strain stored in a refrigerator at 4°C in LB medium at 30°C and 150rpm to expand the culture, use sterile water to prepare a bacterial suspension with an OD of about 1.5, and then inoculate at a 2% inoculation amount Mix well in a 250mL Erlenmeyer flask containing 100mL heterotrophic nitrification medium, and culture at 30°C, 150rpm, on a shaker for 96h. Samples were taken regularly to determine the NH in the supernatant 4 + -N,OD 600 value. In order to reduce the error, three parallel experiments were set up, and the results are shown in image 3 .
[0061] According to the curve, it can be seen that the SLY2-21 bacteria enters the logarithmic phase after 12 hours of growth in the nitrification medium, enters the stable phase after about 27 hours, and enters the decline phase after a short period of stable phase. During strain growth while NH 4 + -N concentration dec...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More