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Degerming method for culturing chlamydomonas reinhardtii through mixed antibacterial agent

An antibacterial agent and rhinestone technology, which is applied in the field of microorganisms, can solve the problems of bacterial contamination and other problems, and achieve the effect of facilitating preservation, simple genetic analysis and biochemical analysis, and simple structure

Active Publication Date: 2019-05-31
XUZHOU NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is to provide a method for removing bacteria in the cultivation process of Chlamydomonas reinhardtii by using mixed antibacterial agents, so as to effectively solve the problem of bacterial contamination in the experiment and application of Chlamydomonas

Method used

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  • Degerming method for culturing chlamydomonas reinhardtii through mixed antibacterial agent
  • Degerming method for culturing chlamydomonas reinhardtii through mixed antibacterial agent
  • Degerming method for culturing chlamydomonas reinhardtii through mixed antibacterial agent

Examples

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Embodiment 1

[0028] Example 1: A. Prepare TAP solid medium for later use; B. Streak inoculate Chlamydomonas polluted by mixed bacteria on the TAP solid medium plate, at a temperature of 23±0.5°C, with a light cycle of 14 / 10h light and dark light , cultivated for 3-5 days under the condition of a light intensity of 8000 Lx; C. Prepare a mixed antibacterial TAP solid medium plate containing 100 μg / ml boscalid, 500 μg / ml ampicillin and 100 μg / ml cefotaxime, and set aside ; D. After the culture of Chlamydomonas polluted by miscellaneous bacteria is completed, it is transferred to the TAP solid medium plate containing mixed antibacterial agents, under the conditions of temperature 23±0.5°C, light cycle 14 / 10h light and dark light, and light intensity 8000Lx Cultivate for 5-8 days to remove miscellaneous bacteria; E. Pick the sterilized monoclonal Chlamydomonas, transfer and streak on a common TAP solid medium plate, at a temperature of 23±0.5°C, and a light cycle of 14 / 10h light and dark Light,...

Embodiment 2

[0035] Referring to the method of Example 1 in CN108485982A, when using the 7th time, the combination of miscellaneous bacteria (Kysstrobin, ampicillin and cefotaxime) has corresponding resistance, such as image 3 shown.

Embodiment 3

[0037] After the Chlamydomonas degerming through embodiment 2, continue to sterilize again with the method of embodiment 1, the result is as follows Figure 4 .

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Abstract

The invention relates to a degerming method for culturing chlamydomonas reinhardtii through a mixed antibacterial agent. According to the method, the mixed antibacterial agent composed of boscalid, ampicillin and cefotaxime is used for removing fungi and bacteria in the chlamydomonas reinhardtii culturing process. The method has the advantages that the boscalid and the cefotaxime are used for removing the fungi, the ampicillin is used for removing the bacteria, through effective cooperation application of the three components, the wide spectrality is achieved, the problem of bacterium contamination generated in the experiment and application process through the chlamydomonas reinhardtii is solved, and the valuable and rare algae filament can be well preserved.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to a method for eliminating bacteria in the cultivation process of Chlamydomonas reinhardtii by using mixed antibacterial agents. Background technique [0002] Chlamydomonas reinhardtii, referred to as Chlamydomonas, is a haploid eukaryotic cell with the characteristics of simple structure, short growth cycle, and easy cultivation. An important model organism in the research fields of cytoskeleton, anaerobic metabolism and biohydrogen production, nutrition and energy metabolism. At present, the whole genome of Chlamydomonas has been sequenced, and the functional analysis of its related genes is also being studied in depth. The development of gene editing technology has further promoted the research of Chlamydomonas as a model organism, and has practical application value, such as cilia disease, biodiesel , Biological decontamination, etc. However, in the process of cultiv...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12R1/89
Inventor 王亮温馨陈卓雅王雯陆军郑元林
Owner XUZHOU NORMAL UNIVERSITY