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Preparation method of inonotus obliquus non sugar extractives and application thereof

A technology of Inonotus obliquus and its extract, which is applied in the field of preparation of non-sugar extracts, can solve the problems of lack of extraction and preparation methods of non-sugar active ingredients, achieve excellent antibacterial function, less types, and simple and easy operation Effect

Inactive Publication Date: 2019-05-31
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In foreign countries, the application of Inonotus obliquus appeared in Russia and Poland early, and was used to treat various intractable diseases, such as cancer, diabetes, etc. At present, there have been many reports on the extraction and application of chemical components of Inonotus obliquus , mainly focusing on the anti-tumor, blood sugar-lowering, and blood-lipid-lowering research of polysaccharides of Inonotus obliquus. Due to the variety of active ingredients in Inonotus obliquus and high application value, current research in this field has not fully utilized and The value of developing the non-sugar active ingredients of Inonotus obliquus is also lacking the extraction and preparation method of the corresponding non-sugar active ingredients

Method used

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  • Preparation method of inonotus obliquus non sugar extractives and application thereof
  • Preparation method of inonotus obliquus non sugar extractives and application thereof
  • Preparation method of inonotus obliquus non sugar extractives and application thereof

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Embodiment 1

[0028] The preparation of the non-sugar extract of embodiment 1 Inonotus obliquus

[0029] 1. Cultivation and fermentation of Inonotus obliquus

[0030] Medium formula: birch powder 5%, 10% glucose, 1.5% peptone, 0.2% KH 2 PO 4 , 0.2% MgSO 4 , and the rest is water. Autoclave the prepared liquid culture medium.

[0031] Inoculate the mycelium of Inonotus obliquus into 50ml of liquid medium, and vibrate at 150rpm / min at 25°C for 1-2 days to obtain seed liquid, and inoculate the seed liquid into 300ml of In the culture medium, shake culture at 150 rpm / min at 25° C. for 5-7 days to obtain a culture solution.

[0032] 2. Cold induction of Inonotus obliquus

[0033] The above-mentioned Inonotus obliquus culture solution was placed in a refrigerator at 4°C for cold induction for 20-40 days. Preferably, the cold induction time is 30 days. This step helps Inonotus obliquus to induce the expression of the target product in extreme environments.

[0034] 3. Preparation of freez...

Embodiment 2

[0046] Antibacterial experiment of embodiment 2 Inonotus obliquus non-sugar extract

[0047] 1. Cultivation of indicator bacteria:

[0048] Escherichia coli, Staphylococcus aureus, Salmonella typhimurium and Shigella were inoculated in beef extract peptone base respectively, and cultured with shaking at 37°C; Candida albicans was inoculated in PDA medium, and cultured at 28°C Shake the culture to obtain the bacterial liquid respectively.

[0049] 2. Setting of sample group:

[0050] Set up the experimental group and the control group respectively according to the following method, and set up at least 3 repetitions for each group:

[0051] Experimental group 1: The chloroform extract of Inonotus obliquus was prepared according to the above-mentioned complete experimental procedures as a sample; Experimental group 2: The fermentation supernatant that was fermented and cold-induced and filtered to remove Inonotus obliquus cells was concentrated at low temperature Finally, it w...

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Abstract

The invention discloses a preparation method of inonotus obliquus non sugar extractives and application thereof. The preparation method comprises the steps of culturing and fermenting inonotus obliquus, performing cold induction treatment on a culture solution, preparing freeze-drying powder, performing chloroform extraction on the freeze-drying powder to obtain a preliminary extracting solution,then, dialysis treatment is performed, finally, high performance liquid chromatography is performed, and purification is performed to obtain a refined extractive. The preparation method is simple andshort in period, the main ingredients of the prepared non sugar extractives are triazine derivatives, the good bacteriostat effect is achieved for bacteria and fungi, the extractives are biological preparation, little environment contamination is generated, and the method can be applied to preparing environment-friendly bactericide and bacteriostat medicine, the good application prospect is achieved, the application value for inonotus obliquus is sufficiently developed, and the utilization rate for medicinal fungi is increased.

Description

technical field [0001] The invention relates to a medicinal fungus extract, in particular to a preparation method and application of a non-sugar extract of Inonotus obliquus. Background technique [0002] At present, Inonotus obliquus, also known as Chaga, is a high-value medicinal fungus belonging to Basidiomycotina, Hymenomycetes, Aphyllophorales, Hymenoehaetaeeae. It is cold-loving and is a wood-rot fungus distributed in the frigid zone. It grows under the bark of living trees such as birch, silver birch, elm, and alder or on the dry trees after felling, and can cause birch, silver birch, elm, and alder. white rot. It is mainly distributed in the northern hemisphere at 45°-50° north latitude, such as northern North America, Finland, Poland, Russia, Heilongjiang in China, Changbai Mountain in Jilin Province, Hokkaido in Japan, etc. [0003] In foreign countries, the application of Inonotus obliquus appeared in Russia and Poland early, and was used to treat various intra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/12A61K31/53C07D251/22A61P31/04A61P31/10C12R1/645
Inventor 陆秀华徐丽丽郭立忠肖淑芹
Owner QINGDAO AGRI UNIV