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Method for preparing dextran selenium polymers by enzyme means

A technology of dextran and polymer, applied in the field of preparation of selenized polysaccharide, can solve the problems of low yield, severe reaction conditions and high product activity

Active Publication Date: 2019-06-07
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the synthesis methods of selenium polysaccharides have their limitations. For example, the reaction conditions of chemical synthesis are relatively severe and the reaction steps are many; the plant transformation method has a long production cycle and low yield, but the activity of the product is high; It is difficult to separate and purify the product, but the obtained selenium polysaccharide is relatively stable and not easy to decompose

Method used

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  • Method for preparing dextran selenium polymers by enzyme means
  • Method for preparing dextran selenium polymers by enzyme means
  • Method for preparing dextran selenium polymers by enzyme means

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The operation steps, conditions and parameters are basically the same as the above method, wherein the concentration of sucrose solution is 0.4mol / L, the enzyme activity of dextran sucrase is 0.4U / mL, Na 2 SeO 3 The concentration is 0.5g / L.

Embodiment 2

[0043] The operation steps, conditions and parameters are basically the same as the above method, wherein the concentration of sucrose solution is 0.4mol / L, the enzyme activity of dextran sucrase is 0.4U / mL, Na 2 SeO 3 The concentration is 1.0g / L.

Embodiment 3

[0045] The operation steps, conditions and parameters are basically the same as the above method, wherein the concentration of sucrose solution is 0.4mol / L, the enzyme activity of dextran sucrase is 0.4U / mL, Na 2 SeO 3 The concentration is 2.0g / L. Example 4

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Abstract

The invention discloses a method for synthetizing dextran selenium polymers by an enzyme means. According to the method for synthetizing the dextran selenium polymers by the enzyme means, dextran sucrase is used as a catalyst; sucrose and sodium selenite are used as substrates; and then, enzymatic reaction is carried out so as to synthesize the dextran selenium polymers. By catalyzing the sucroseand the sodium selenite by using the dextran sucrase, the method is capable of synthesizing the dextran selenium polymers in one step. Tests have proven that the method for synthesizing the dextran selenium polymers by the enzyme means overcomes the problems of complex reaction steps, intense reaction conditions, relatively many byproducts and the like of chemical synthesis; moreover, the method has the characteristics of being simple in reaction system, wide in raw material source, mild in reaction conditions, relatively high in selenization efficiency, relatively high in selenium polymer yield and the like. In addition, the method is capable of reducing usage of toxic chemical agents as reaction media; moreover, relatively few byproducts are produced so as to facilitate separation and purification, so that, no environmental pollution is caused.

Description

technical field [0001] The invention belongs to the technical field of selenized polysaccharide preparation, in particular to a method for enzymatically preparing dextran-selenopolymer. Background technique [0002] Selenium is one of the trace elements necessary to maintain the normal physiological functions of the human body and animals. It plays an important role in improving the body's immunity, protecting the nervous system, anti-oxidation, and anti-tumor. Inorganic selenium mainly exists in natural environments such as soil, water, and the atmosphere, and generally refers to monomeric selenium and inorganic compounds such as selenous acid and selenite. Its residence time in the body is relatively short, and excess selenium will be quickly excreted out of the body with urine, with low biocompatibility and strong toxic and side effects. Organic selenium mainly exists in animals and plants, mainly including organic compounds such as selenium polysaccharides, selenium ami...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12P19/04
Inventor 陈山齐鹏翔王帅静黄双霞王清陈玉颖徐欣东慈璐雨蓝尉冰
Owner GUANGXI UNIV
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