Nano gold array surface-enhanced Raman chip, preparation method thereof and application

A surface-enhanced Raman and nano-gold technology, which is applied in Raman scattering, preparation of test samples, material excitation analysis, etc., can solve the problems of inconvenient transfer detection, poor stability, and limited enhancement performance of surface-enhanced Raman materials. , to achieve the effect of simplifying the pre-processing process, efficient transfer and avoiding interference

Inactive Publication Date: 2019-06-18
HUAZHONG UNIV OF SCI & TECH RES INST SHENZHEN +1
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  • Abstract
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Problems solved by technology

[0003] The invention solves the technical problems of limited enhancement performance, poor stability, and inconvenient transfer detection of surface-enhanced Raman materials for non-destructive rapid detection in the prior art, and provides a surface-enhanced Raman chip and its preparation method and Applications, especially a surface-enhanced Raman chip based on assembled nano-gold, and its rapid detection method for surface extraction and coordination transfer of pesticide residues in fruits and vegetables

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  • Nano gold array surface-enhanced Raman chip, preparation method thereof and application
  • Nano gold array surface-enhanced Raman chip, preparation method thereof and application
  • Nano gold array surface-enhanced Raman chip, preparation method thereof and application

Examples

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Embodiment 1

[0034] Take 50mL of chloroauric acid aqueous solution with a mass fraction of 0.4% and place it in a three-necked flask, heat it in an oil bath at 100°C to make it boil, and then add 0.1mol L -1 1mL of sodium citrate, and start timing, continue to react for 30min, turn off the heating and continue to stir until the solution is cooled to room temperature, and obtain a gold nanoparticle dispersion (gold particle diameter is about 50nm, and the shape is spherical). A nylon organic filter membrane (pore size of 0.22 μm) was placed in a vacuum filtration device, 5 mL of the prepared nano-gold dispersion was added thereto, and 5 mL of cyclohexane was added. Add 2mL of absolute ethanol to the aqueous phase dispersion at the phase interface or by injection, and a nano-gold array assembled by nano-gold can be obtained at the phase interface. Start the vacuum filtration pump to transfer the nano-gold array to the surface of the organic solvent-resistant flexible filter membrane. The fil...

Embodiment 2

[0036] figure 2 It is a schematic diagram of the determination process of pesticide residues on the surface of fruits and vegetables by the surface-enhanced Raman chip proposed by the present invention. The method proposed by the present invention is used to measure thiram on the surface of apples: 5 μL of thiram ethanol solution with different concentrations is added dropwise to the surface of apples, and blown dry with nitrogen. Take 5 mL of methanol and mix with 5 mL of an aqueous solution with a pH of 8 to obtain an extraction solvent. Take 5 μL of the extraction solvent and drop it on the surface of the apple dripped with thiram, then take the surface-enhanced Raman chip obtained in Example 1 and attach it to the same position of the apple, so that the thiram bimolecule is transferred to the chip surface, and then placed in a portable Raman spectrometer The measurement was carried out under the following conditions, using a 785nm laser as the light source, the laser ene...

Embodiment 3

[0039] Adopt the method proposed by the present invention to measure the multiple pesticide residue molecules on the apple surface: get 5ppm of parathion, methyl parathion, fenthion, phoxim, chlorpyrifos, methyl chlorpyrifos, cypermethrin and flucyvalol Add 5 μL of the methanol solution of permethrin dropwise to the surface of the apple, and dry it with nitrogen. Take 5 mL of acetone and mix with 5 mL of aqueous solution with pH 7 to obtain an extraction solvent. Take 5 μL of the extraction solvent and add it dropwise to the surface of the apple dripped with pesticide residues, then take the assembled nano-gold surface-enhanced Raman chip obtained in Example 1 and attach it to the same position of the apple, so that the pesticide residue molecules can be transferred to the surface of the chip, and then placed in a portable The measurement was carried out under a Raman spectrometer, using a 785nm laser as the light source, the laser energy was selected as 10mW, the exposure tim...

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Abstract

The invention relates to a nano gold array surface-enhanced Raman chip, a preparation method thereof and application of the Raman chip and belongs to the field of rapid detection of pesticide residues. According to the preparation method of the chip, a nano gold array is formed by means of the assembling of the interface of nano gold; the array is transferred to the surface of a flexible filteringmembrane, the flexible filtering membrane with the array is dried and cut into a structure of a suitable size, so that the surface-enhanced Raman chip can be obtained; a solvent is added to the surface of a fruit or vegetable to be tested, so that pesticide residues are extracted into the solvent; and the chip is attached to the surface of the fruit or vegetable and is made to wipe the solvent, so that pesticide residue molecules are transferred to the surface of the chip through coordination, and then a Raman spectrometer is used to quantitatively measure the pesticide residue molecules on the chip. The chip of the invention has high surface strengthening capacity; the surface extraction method and coordination transfer method are adopted, and therefore, the introduction of the interference of the complicated matrixes of the fruit or vegetable to be tested can be effectively avoided, operation is simple and convenient, and the surface extraction method and coordination transfer method can be conveniently used for transferring the pesticide residues on the surface of the fruit or vegetable; and the chip and the portable Raman spectrometer can be used jointly to achieve the quantitative detection of the pesticide residues on the fruit or vegetable.

Description

technical field [0001] The invention belongs to the field of rapid detection of pesticide residues, and relates to a nano-gold array surface-enhanced Raman chip and its preparation method and application, in particular to a surface-enhanced Raman chip based on assembled nano-gold, and its use for surface extraction of pesticide residues in fruits and vegetables and a rapid detection method for coordination transfer. Background technique [0002] In order to increase the yield of agricultural products and reduce the losses caused by pests and diseases, pesticides are widely used in modern agriculture. The unreasonable use of pesticides, such as the use of banned or restricted pesticides, or the misuse of unacceptable metering or safety intervals, has caused serious residue problems in agricultural products such as fruit trees. In order to ensure the safety of food materials, it is necessary to conduct accurate and rapid detection of pesticide residues in agricultural product...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65G01N1/40
Inventor 朱丽华欧阳磊姚玲
Owner HUAZHONG UNIV OF SCI & TECH RES INST SHENZHEN
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