Application and preparation method of metastatic encephaloma targeted nano drug delivery system with effects of LRP1 up-regulation and targeting
A technology of brain metastases and drug delivery system, applied in the field of nanobiomedicine
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[0067] A preparation method of a brain metastases-targeted nano drug delivery system that can upregulate and target LRP1, comprising:
[0068] Step 1: Dissolve the biodegradable polymer base carrier in an appropriate amount of organic solvent, and add a certain amount of functional small molecules that can up-regulate LRP1 to form an oil phase;
[0069] In one embodiment, this step can be specifically performed as follows: the biodegradable polymer base carrier is dissolved in an appropriate amount of organic solvent, and a certain amount of functional small molecules that can up-regulate LRP1 is added to form an oil phase, wherein the The polymer materials mentioned are polylactic acid (molecular weight of 0.3-15,000, 15-30,000, 3-55,000, 55-90,000), polylactic acid-glycolic acid (molecular weight of 0.1-15,000, 15-24,000, 2.4-3.8 million, 3.8-5.3 million, 5.3-70,000, 7-88,000, the ratio of lactide and glycolide is 75 / 50 or 50 / 50), polycaprolactone (molecular weight is 0.4-40...
Embodiment 1
[0095] Weigh 50 mg of polylactic acid-glycolic acid-polylysine and 50 mg of simvastatin and dissolve them in 1 mL of ethyl acetate as the oil phase. Weigh 25 mg of doxorubicin hydrochloride and dissolve in 100 μL of water. As the inner water phase, the solution was added dropwise to the oil phase under vortexing conditions to form a water-in-oil emulsion; then the emulsion was added dropwise to 4 mL of 2.5% polyvinyl alcohol solution under vortexing conditions, and phacoemulsification was performed A water-in-oil-in-water emulsion is formed; the emulsion is quickly poured into 50 mL of a 0.3% polyvinyl alcohol solution, and the ethyl acetate is removed by stirring and volatilization; the obtained nanoparticle suspension is centrifuged at a low speed of 1000 rpm for 5 min to obtain a supernatant to remove larger nanoparticles particles and unencapsulated simvastatin; the aforementioned supernatant was subjected to ultracentrifugation at 30,000 rpm for 20 min to obtain nanopartic...
Embodiment 2
[0097] Weigh 50 mg of polylactic acid-glycolic acid-polylysine and 50 mg of simvastatin and dissolve them in 1 mL of ethyl acetate as the oil phase. Weigh 25 mg of doxorubicin hydrochloride and dissolve in 100 μL of water. As the inner water phase, the solution was added dropwise to the oil phase under vortexing conditions to form a water-in-oil emulsion; then the emulsion was added dropwise to 4 mL of 2.5% polyvinyl alcohol solution under vortexing conditions, and phacoemulsification was performed A water-in-oil-in-water emulsion is formed; the emulsion is quickly poured into 50 mL of a 0.3% polyvinyl alcohol solution, and the ethyl acetate is removed by stirring and volatilization; the obtained nanoparticle suspension is centrifuged at a low speed of 1000 rpm for 5 min to obtain a supernatant to remove larger nanoparticles particles and unencapsulated simvastatin; the aforementioned supernatant was subjected to ultracentrifugation at 30,000 rpm for 20 min to obtain nanopartic...
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