Acinetobacter and application thereof

A bacillus and strain technology, applied in the field of Acinetobacter and its wastewater treatment, can solve the problems of being easily affected by the external environment, long generation cycle of autotrophic bacteria, difficult to operate stably for a long time, etc., to achieve shortened start-up time and strong tolerance sex, avoid slow growth effects

Inactive Publication Date: 2019-06-21
BEIJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of these biological treatment processes rely on autotrophic aerobic / anaerobic bacteria. Due to the shortcomings of autotrophic bacteria, such as long generation cycle, slow growth, poor impact resistance, and easy to be affected by the external environment, the traditional biological denitrification process is difficult to stabilize for a long time. run

Method used

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  • Acinetobacter and application thereof
  • Acinetobacter and application thereof
  • Acinetobacter and application thereof

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Experimental program
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Effect test

Embodiment 1

[0026] The screening of embodiment 1 Acinetobacter (Acinetobacter) JQ1004

[0027] The strain was isolated from an activated sludge sample of a pilot plant of Beijing Gaobeidian Sewage Treatment Plant.

[0028] (1) Configuration of culture medium

[0029] Heterotrophic nitrification medium (g / L): 0.47 (NH 4 ) 2 SO 4 ,4.219 (CH 2 COONa) 2 ·6H 2 O, 50mL Vickers salt solution, C / N=7.5.

[0030] Denitrification medium (g / L): 0.72KNO 3 ,4.219 (CH 2 COONa) 2 ·6H 2 O, 50mL Vickers salt solution, C / N=7.5.

[0031] Vickers salt solution (g / L): 6.55K 2 HPO 4 ·3H 2 O,2.5MgSO 4 ·7H 2 O, 2.5NaCl, 0.038MnSO 4 ·H 2 O,0.05 FeSO 4 ·7H 2 O.

[0032] LB medium (g / L): 10.00 tryptone, 5.00 yeast extract, 10.00 NaCl.

[0033] C / N in the above medium refers to TC / TN (w / w) in the medium.

[0034] (2) Isolation of strains

[0035] The target strain was isolated by serial dilution method. Add 10mL of activated sludge homogeneous solution into 90mL of 0.9% sterile saline, and us...

Embodiment example 2

[0036] Implementation Case 2 Identification of 16S rDNA of Acinetobacter JQ1004

[0037]Pick a single colony that grows well on the plate medium, inoculate it in a heterotrophic nitrification medium, and culture it at 160r / min at 30°C for 12h to obtain a bacterial suspension. Take a certain amount of bacterial liquid and centrifuge at 10000r / min at 4°C for 5min to obtain bacterial cells. The bacteria were resuspended in sterile phosphate buffer solution, centrifuged again to obtain the bacteria, and repeated three times to clean the impurities in the bacteria. Add 400 μL Buffer SCL to the cells, bathe in water at 65°C for 1 hour, and mix once every 10 minutes until the mixture is clear, indicating that the cells have been completely lysed. The supernatant was then centrifuged to extract the strain DNA. DNA was extracted using Ezup Column Genomic DNA Extraction Kit (Shanghai Sangong), and the specific steps were referred to the instructions. Using the extracted DNA as a temp...

Embodiment example 3

[0038] Implementation Case 3 Determination of Heterotrophic Nitrification and Aerobic Denitrification Performance of Acinetobacter JQ1004

[0039] Inoculate a strong single colony into sterile 0.9% normal saline water and make it to a constant volume, prepare a uniform bacterial suspension as the inoculum, and fix the OD of the inoculum 600 The value is about 0.5, and the inoculation amount in the experiment is 2% (v / v). The heterotrophic nitrification medium and denitrification medium with ammonium sulfate and potassium nitrate as the only nitrogen source were respectively configured to explore the denitrification performance of the strains under different nitrogen sources. The C / N of the medium was set at 7.5, the pH was set at 7.0-7.1, the culture conditions were 30°C, 160r / min. Samples were taken regularly to measure the concentration of ammonia nitrogen, nitrite nitrogen and nitrate nitrogen. Get bacterial strain to the removal law of ammonia nitrogen and nitrate nitroge...

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Abstract

The invention discloses acinetobacter and application thereof. The acinetobacter is named as Acinetobacter JQ1004, and the Acinetobacter strain JQ1004 was deposited in China Microbiological Culture Collection Management Committee (CGMCC) on March 06, 2018 under the accession number of CGMCC No. 15414. The acinetobacter is a heterotrophic nitrifying aerobic denitrifying bacterium, can carry out nitrifying and denitrifying by taking organic carbon as a carbon source under the same aerobic condition, and can respectively grow by taking ammonia nitrogen and nitrate nitrogen as nitrogen sources. The acinetobacter disclosed by the invention has the characteristic of high-concentration ammonia nitrogen load resistance, and can be propagated and metabolized under the condition that the ammonia nitrogen concentration is 0-2000 mg/L.

Description

technical field [0001] The invention relates to a strain of Acinetobacter and its application in waste water treatment. The strain is a heterotrophic nitrifying aerobic denitrifying bacteria, which can grow and metabolize with organic carbon as carbon source and ammonia nitrogen or nitrate nitrogen as nitrogen source under aerobic conditions. In addition, the strain has a strong tolerance to high concentrations of ammonia nitrogen, so it has great application potential in the field of treatment of high concentrations of ammonia nitrogen organic wastewater. Background technique [0002] The treatment of high ammonia nitrogen wastewater has become a difficult point in the field of wastewater treatment, especially in the wastewater treatment of ammonia nitrogen pollution units. The discharge of high-ammonia-nitrogen wastewater mainly comes from petrochemical, coking, synthetic ammonia, and tanning industries. The concentration of ammonia nitrogen in some wastewater will exceed...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/38
Inventor 李军王秀杰王维奇张晶张阳
Owner BEIJING UNIV OF TECH
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