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A kind of colorimetric detection method of primaquine drugs

A technology of primaquine and detection method, applied in the field of medicine, can solve the problems of interfering with PMQ determination and the like

Active Publication Date: 2022-05-24
GUANGZHOU UNIVERSITY OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, many endogenous biomolecules such as amino acids, vitamins, nucleic acids, and urochrome have strong absorption near 260 nm, which will interfere with the determination of PMQ

Method used

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  • A kind of colorimetric detection method of primaquine drugs
  • A kind of colorimetric detection method of primaquine drugs
  • A kind of colorimetric detection method of primaquine drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 Grimess reaction of primaquine

[0035]Dissolve the aniline and 1-fold primaquine (PMQ) in 5% phosphoric acid, followed by the slow addition of sodium nitrite at room temperature (25 °C). The formation of colored azo products can be easily monitored by naked eye observation or TLC (e.g., t.g., TLC Figure 1 )。

[0036] The reaction is carried out using aniline with various substituents in the benzene ring. Changing the substituent group can form products of different colors. as Figure 2 As shown, substitutions with electron-giving effects can essentially cause redshifts in the UV-Vis absorption spectrum. The spectrum of the strongly powered methoxy-substituted reaction product 3d has the strongest absorption at 504 nm, while the spectrum of the product 3a of strongly absorbed electron sulfonamide has the strongest absorption at 470 nm. The power supply of methyl groups is lower than that of methoxy, but it is still much stronger than sulfonamides, nitro groups and ...

Embodiment 2

[0042] Example 2 tests the change in absorbance and time of the colored product

[0043] PMQ can react with various anilines to form colored products, and then use this color development reaction to determine PMQ colorimetric. Tested with aniline with different electrical substituents (R) (sulfonyl 2a, methyl 2c and methoxy 2d). To test absorbance versus change over time, first mix 100 μL of aniline solution (200 mM dissolved in 0.2 M HCl) with 50 μL of PMQ (50 μM) and then add 50 μL of aqueous sodium nitrite (5 mM). The dynamic change in absorbance of each aniline at maximum wavelength is recorded by a multifunctional microplate reader.

[0044] as Figure 3 As indicated, the reaction in 2a is faster than the other two anilines. The reaction rate of 2c is higher than 2d but below 2a, mainly because the electron-giving effect of methyl is somewhere in between. As mentioned earlier, the same trend was observed on the displacement of the UV-Vis absorption peak. What's more, PMQ itsel...

Embodiment 3

[0046] Example 3Griess reaction to selectivity of PMQ

[0047] The antimalarial drugs 4-aminoquinoline mefloquine (MQ), chloroquine (CQ), piperaquine (PIP) and dihydroartemisinin (DHA) were used to participate in the Griess reaction alone, or other antimalarial drugs were mixed with PMQ to participate in the reaction. The results are as follows Figure 5 as shown, absorbance value I 504 There was no response to MQ, CQ, PIP, and DHA (all 50 μM), but to drug mixtures containing PMQ (50 μM). The results showed that the Grices-based response method was highly selective for PMQ and could distinguish between other antimalarial drugs commonly used in malaria combinations.

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Abstract

The invention relates to a colorimetric detection method for primaquine drugs. Under the conditions of an acidic environment and the presence of nitrite, aniline with substituents at the para-position can undergo a Griess reaction with primaquine to form a colored azo product; Determine the UV-Vis absorption spectrum and the maximum absorbance value of the above-mentioned colored product; under the presence of different concentrations of primaquine detected by a microplate reader, the formation of the above-mentioned colored azo product and the change of its absorbance value; establish the primaquine concentration and azo The standard curve of the product absorbance value; through the corresponding relationship between the concentration of primaquine on the standard curve and the absorbance value of the azo product, the concentration of the primaquine drug in the sample to be tested is determined. This method has been successfully used to quantify the concentration of primaquine in synthetic urine with a detection limit as low as 0.63 μM. The method also detects primaquine from serum samples in a clinically relevant concentration range.

Description

Technical field [0001] The present invention relates to the field of medicine, in particular to a colorimetric detection method of a primaquine drug. Background [0002] Primaquine (PMQ), an 8-aminoquinoline structure, is the only antimalarial drug licensed for the control of Plasmodium vivax and Plasmodium ovale, and the only potent drug capable of effectively killing Plasmodium malaria in the gametophyte phase. However, the side effect of PMQ is that it often leads to acute hemolytic anemia in patients with glucose-6-phosphate dehydrogenase (G6PD) deficiency, and the severity of hemolysis depends on the degree of G6PD deficiency, PMQ dose, and duration. The frequency of G6PD gene mutations is 3-30% in malaria-endemic areas and is widely distributed, thus limiting the widespread use of PMQ. Although the World Health Organization recommends a single low dose (0.25 mg / kg) to stop malaria transmission while reducing the risk of hemolysis, treatment for malaria still faces the varia...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78G01N21/31
Inventor 刘芳邓涛伍盛鋆吴亚兰
Owner GUANGZHOU UNIVERSITY OF CHINESE MEDICINE
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