A Sandwich Prostate Specific Antigen Photoelectrochemical Detection Method Based on In Situ Signal Amplification
A prostate-specific, signal-amplifying technology, applied in the direction of material electrochemical variables, measuring devices, scientific instruments, etc., can solve problems such as long detection time
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Embodiment 1
[0024] A TiO-based 2 The preparation method of the photoelectrochemical sensor of mesoscopic crystal (such as figure 1 shown):
[0025] (1) Pretreatment of the glassy carbon electrode: the glassy carbon electrode is first mechanically polished on the suede covered with alumina powder, washed with secondary water to remove the residual powder on the surface, and then moved into an ultrasonic water bath for cleaning until it is cleaned. Wash thoroughly with ethanol, dilute acid and water;
[0026] (2) Au / Ab 1 / PSA modified electrode preparation: drop 3 μL of gold cone (Au NCs) solution with a concentration of 2 mg / mL on the surface of a clean glassy carbon electrode, dry it under infrared light, and cool to room temperature; then add 3 μL of a concentration of 5 mM mercaptophenylboronic acid solution was drop-coated on the surface of the modified electrode, incubated at 4 °C for 50 min, washed with secondary water to remove excess solution, and the modified electrode was adde...
Embodiment 2
[0029] TiO used in embodiment 1 2 - Preparation of B material:
[0030] TiO 2 -Preparation of B nanorods were synthesized by solvothermal method using titanium nanowires as precursors. 1 g TiO 2 Disperse in 75 mL of 15 M KOH in water. After shaking for 10 minutes, the obtained suspension was transferred to a 100 mL reactor, and the reactor was placed at 170 °C for 72 h, and then cooled to room temperature. The resulting deposit was washed with acetic acid solution until the pH was 7.0. The final product was collected by centrifugation and dried in a forced-air oven at 60 °C for 12 h to make TiO 2 Nanowire precursors. TiO 2 -Synthesis of B, first 300 mg of TiO 2 The nanowire precursor was dispersed in 50 mL of 8 M acetic acid solution, then the solution was transferred to a reaction kettle, heated to 180 °C, and reacted for 24 h. The obtained product was collected by centrifugation, washed with secondary water and ethanol, and dried overnight at 60 °C.
[0031] The pr...
Embodiment 3
[0034] A TiO-based 2 -The photoelectrochemical sensor of B crystal is used for the detection method of prostate specific antigen, and the steps are as follows:
[0035] (1) Using a three-electrode system for measurement, the Au / Ab prepared in Example 1 1 / PSA / Ab 2 / Ag 2 The S-modified electrode was used as the working electrode, Ag / AgCl was used as the reference electrode, and the platinum wire electrode was used as the counter electrode. The photoelectrochemical workstation was used for detection. The voltage was set at -0.1 V, and the lamp was switched on and off every 10 s. The photoexcitation light source is filtered by a monochromator before use;
[0036] (2) In PBS buffer solution with pH 7.4, detect 1×10 by photoelectrochemical workstation -6 ng / mL–50 ng / mL series of PSA standard solutions with different concentrations, and draw the working curve by recording the different current signals generated before and after switching the light. Figure 2A 1×10 for differen...
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