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Culture medium and preparation method thereof

A technology of culture medium and purple potato starch, applied in the biological field, can solve problems such as hidden dangers of host health

Active Publication Date: 2019-06-28
INNER MONGOLIA MENGNIU DAIRY IND (GRP) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently, most of the medium used for the isolation of bifidobacteria contains one or more of antibiotics such as mupirocin, sodium propionate, neomycin sulfate, paromomycin, thiamycin, and thiolongomycin. , it is easy to enhance the drug resistance of the strain during the isolation process, and the antibiotic resistance gene carried by the bifidobacteria may be transferred to the pathogenic bacteria in the host intestinal tract, bringing hidden dangers to the host's health

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] (1) The formulation of the separation medium is (included per liter):

[0037] Purple potato starch 10g,

[0038] Freeze-dried purple potato anthocyanins 1g,

[0039] Tryptone 15g,

[0040] Casein Phosphopeptide 5g,

[0041] Tween 80 1mL,

[0042] Soybean oligosaccharides 15g,

[0043] Sodium chloride 5g,

[0044] Lithium chloride 3g,

[0045] Agar 15g,

[0046] Distilled water to make up to 1000mL.

[0047] (2) The culture medium preparation method comprises the following steps:

[0048] 1. Weigh purple potato starch according to the formula, dissolve it in distilled water at 75-80°C, stir to dissolve and gelatinize,

[0049] 2. Add tryptone, casein phosphopeptide, Tween 80, soybean oligosaccharides, sodium chloride, lithium chloride and agar to the starch gelatinization liquid,

[0050] 3. Distilled water to 1000mL,

[0051] 4. Adjust the pH to 6.5-7.0,

[0052] 4. The medium is sterilized by autoclaving at 115°C for 15 minutes, and then cooled to 40-45°C ...

Embodiment 2

[0058] (1) The formulation of the separation medium is (included per liter):

[0059] Purple potato starch 20g,

[0060] Freeze-dried purple potato anthocyanins 2g,

[0061] Tryptone 10g,

[0062] Casein Phosphopeptide 8g,

[0063] Soybean oligosaccharides 5g,

[0064] Sodium chloride 5g,

[0065] Lithium chloride 3g,

[0066] Agar 15g,

[0067] Distilled water to make up to 1000mL.

[0068] (2) The culture medium preparation method comprises the following steps:

[0069] 1. Weigh purple potato starch according to the formula, dissolve it in distilled water at 75-80°C, stir to dissolve and gelatinize,

[0070] 2. Add tryptone, casein phosphopeptide, Tween 80, soybean oligosaccharides, sodium chloride, lithium chloride and agar to the starch gelatinization liquid,

[0071] 3. Distilled water to 1000mL,

[0072] 4. Adjust the pH to 6.5-7.0,

[0073] 4. The medium is sterilized by autoclaving at 115°C for 15 minutes, and then cooled to 40-45°C for later use.

[0074] ...

Embodiment 3

[0079] (1) The formulation of the separation medium is (included per liter):

[0080] Purple potato starch 15g,

[0081] Freeze-dried purple potato anthocyanins 3g,

[0082] Tryptone 8g,

[0083] Casein Phosphopeptide 10g,

[0084] Tween 80 1mL,

[0085] Soybean oligosaccharides 10g,

[0086] Sodium chloride 5g,

[0087] Lithium chloride 3g,

[0088] Agar 15g,

[0089] Distilled water to make up to 1000mL.

[0090] (2) The culture medium preparation method comprises the following steps:

[0091] 1. Weigh purple potato starch according to the formula, dissolve it in distilled water at 75-80°C, stir to dissolve and gelatinize,

[0092] 2. Add tryptone, casein phosphopeptide, Tween 80, soybean oligosaccharides, sodium chloride, lithium chloride and agar to the starch gelatinization liquid,

[0093] 3. Distilled water to 1000mL,

[0094] 4. Adjust the pH to 6.5-7.0,

[0095] 4. The medium is sterilized by autoclaving at 115°C for 15 minutes, and then cooled to 40-45°C fo...

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Abstract

The invention provides a culture medium. The culture medium is prepared from a bacteriostatic agent which is prepared from salt and anthocyanin. The culture medium does not contain antibiotics, the antibiotics is replaced by the anthocyanin to play role of inhibiting miscellaneous bacteria. Meanwhile, the effect of indicators is achieved by ingeniously utilizing the phenomenon that the anthocyaninproduces acid along with lactic acid bacteria to change color, the indicators such as resazurin, bromcresol purple, bromocresol green, litmus, MTT and TTC are avoided, purple potato starch can promote proliferation of bifidobacteria, and then the bifidobacteria can be easily and safely separated out from feces.

Description

technical field [0001] The invention relates to the field of biology, in particular, the invention relates to a culture medium and a preparation method thereof. Background technique [0002] Probiotics are active microorganisms with beneficial effects that can colonize the intestinal tract through the digestive tract, and bifidobacteria are currently generally recognized probiotics. In the field of food, the Ministry of Health has included bifidobacteria in the "List of strains that can be used in food", "List of strains that can be used in health food" and "List of strains that can be used in infant food". Most of probiotic fermented drinks, probiotic yogurt, probiotic milk powder and milk tablets contain bifidobacteria. [0003] Scientific research has found that there are more than 100 species of bacteria in the digestive tract of a healthy human body, the number of which is more than 100 trillion. The imbalance of the types and proportions of the human intestinal microb...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/38C12N1/02C12R1/01
Inventor 乌云刘红霞母智深李洪亮高增丽杨永龙孙涛
Owner INNER MONGOLIA MENGNIU DAIRY IND (GRP) CO LTD
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