Method for rapidly screening low-temperature and weak-light breeding material of cucumis melo L.var.conomon Group

Abstract

The invention discloses a method for rapidly screening a low-temperature and weak-light breeding material of cucumis melo L.var.conomon Group, and relates to the technical field of genetic breeding. Firstly, embryo fetching conditions of the crisp melons are determined, wherein 16-18 days after flowering and pollination, peeled seeds obtained under the conditions have a high survival rate after being subjected to tissue culture and germinate 10 days earlier than mature seeds; secondly, the best culture medium for the peeled seeds is determined, wherein the culture medium contains 950 mg / L potassium nitrate, 825 mg / L ammonium nitrate, 85 mg / L potassium dihydrogen phosphate, 90 mg / L anhydrous magnesium sulfate, 165 mg / L anhydrous calcium chloride, 0.83 mg / L potassium iodide, 6.2 mg / L boric acid, 16.9 mg / L manganese sulfate monohydrate, 8.6 mg / L zinc vitriol, 0.25 mg / L sodium molybdate dihydrate, 0.025 mg / L copper sulfate pentahydrate, 0.025 mg / L cobalt chloride hexahydrate, 37.26 mg / L ethylene diamine tetraacetic acid disodium, 15.2 mg / L anhydrous ferrous sulfate, 8,500 mg / L agar powder, 18,000 mg / L sucrose and the like; meanwhile, the best conditions for adversity identification when obtained seedlings of cucumis melo L.var.conomon Group are cultured to the stage of cotyledon unfolding are determined, wherein the daytime temperature is 15-18 DEG C, and the night temperature is 12-15 DEG C. By means of the method, the target material can be rapidly screened.

Description

technical field [0001] The invention relates to the technical field of genetic breeding, in particular to a method for rapid screening of low-temperature and weak-light resistant breeding materials of crispy melon. Background technique [0002] In the Jianghuai River Basin, the main facilities for the cultivation of crispy melons are plastic film greenhouses, and multi-layer covering of plastic film greenhouses is also used for winter and spring seedling cultivation. However, the temperature in the winter and spring seasons in the Jianghuai River Basin is relatively low, and the commonly used production facilities have relatively low heat preservation and light supplement capabilities. At the same time, the Jianghuai River Basin often encounters continuous cloudy and rainy weather in winter and spring, and the low temperature and low light adversity are more prominent, which restricts the early-maturing cultivation process of crispy melons in spring. Therefore, it is necessa...

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Problems solved by technology

[0004] The purpose of the present invention is to overcome the deficiencies of the prior art, and provide a method for rapid screening of low-temperature and low-light breeding materials for crispy melons, so as to solve the breeding method cycle for new varieties of crispy melons that are resistant to low temperature and low light in the prior art Technical problems such as too long, poor screening effect, time-consuming and labor-intensive

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