Preparation method for extracting phycoerythrobilin, polysaccharides and dietary fibers from low valued lavers and application of preparation method

A technology of seaweed polysaccharides and dietary fiber, applied to bacteria used in food preparation, polysaccharide/gum-containing food ingredients, applications, etc., can solve the problems of high industrial production costs, complex and cumbersome processes, etc., achieve obvious health effects and enhance curative effect , The preparation process is simple, stable and efficient

Active Publication Date: 2019-07-05
HUZHOU TEACHERS COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Chinese patent (201410697186.0) discloses a method for simultaneously extracting phycoerythrin and laver sulfated polysaccharide from inferior laver. The extraction of phycoerythrin needs to go through two chromatography columns. The process is complicated and cumbersome, and the phycoerythrin and polysaccharide Separate extraction, high cost of industrial production

Method used

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  • Preparation method for extracting phycoerythrobilin, polysaccharides and dietary fibers from low valued lavers and application of preparation method
  • Preparation method for extracting phycoerythrobilin, polysaccharides and dietary fibers from low valued lavers and application of preparation method
  • Preparation method for extracting phycoerythrobilin, polysaccharides and dietary fibers from low valued lavers and application of preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1: Ultra-clinical extraction process of phycoerythrin and laver polysaccharide

[0025] (1) Supercritical extraction process screening

[0026] Put the fresh laver at the end of water into a centrifugal dehydrator to remove water, chop it up, add entrainer and soak it overnight. Divided into two methods of extraction:

[0027] Method I: Put the dried seaweed soaked overnight and entrainer into supercritical CO 2 In the fluid extraction tank, the liquid-solid ratio of the filler is 1:40, the extraction temperature is 32°C, the extraction pressure is 60MPa, and the dynamic extraction is carried out for 2h at the flow rate of the entrainer at 10mL / min.

[0028] Method II: Put the dried seaweed soaked overnight and the entrainer into supercritical CO 2 In the fluid extraction tank, the liquid-solid ratio of the filler is 1:40, the extraction temperature is 32°C, and the extraction pressure is 60 MPa. Static extraction is performed for 1 hour, and then entrainer i...

Embodiment 2

[0045] Embodiment 2: Preparation and purification process of phycoerythrin

[0046] After the extract was concentrated under reduced pressure, 20% ammonium sulfate solution was added, centrifuged at 10000r / min for 20min, and the supernatant was taken to continue to add ammonium sulfate solution to 60%, after standing for 12h, centrifuged at 10000r / min for 20min, the supernatant was used for later use. After the precipitate was reconstituted, it was dialyzed with a dialysis bag with a molecular weight cut-off of 1000 Da until no salt ions existed, and then freeze-dried to obtain purified phycoerythrin with a calculated yield of 2.4%. Measure the absorbance at 280nm and 562nm, and calculate the purity of phycoerythrin to reach 2.1. Figure 5 Shown is the ultraviolet-visible absorption spectrum of phycoerythrin at 400nm-700nm. It can be seen from the figure that phycoerythrin has two complete absorption peaks and one absorption shoulder peak, the absorption peaks are located at ...

Embodiment 3

[0047] Embodiment 3: Hygroscopic and moisturizing activity of phycoerythrin

[0048] Clean and dry the desiccator and weighing bottle, and put the color-changing silica gel in the desiccator. Accurately weigh 0.25g of dried phycoerythrin into a dry weighing bottle, add 1mL of distilled water each, and accurately weigh the total weight. Open the cap of the weighing bottle and place it in a desiccator, seal the desiccator, and put it into a 25°C incubator. Accurately weigh after 2h, 4h, 6h, 12h, 24h, 48h, and 72h, respectively weigh the water content Hn and the added water content H after the sample is placed 0 . Calculate the moisture retention rate according to the following formula: moisture retention rate / %=100Hn / H 0 . . Take 2 desiccators and 10 weighing bottles, clean and dry them, and divide them into two groups, one group is potassium acetate saturated solution group, and the other group is ammonium sulfate saturated solution group. Take an appropriate amount of sa...

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Abstract

The invention belongs to the technical field of marine organism applications, and relates to a preparation method for extracting phycoerythrobilin, polysaccharides and dietary fibers from low valued lavers and an application of the preparation method. According to the method, after the low valued lavers are dewatered, chopped and soaked in entrainers, by the aid of a supercritical CO2 fluid extraction technology, extraction, separation and purification are performed at proper packing liquid-solid ratio, extraction temperature and extraction pressure to obtain the phycoerythrobilin and the laver polysaccharides. Fishy smell removing agents are added into residual algal residues to remove fishy smell, and the residual algal residues are dried, activated and granulated to obtain the laver dietary fibers. The preparation process is simple, stable, green and efficient, and the obtained three products are fine in powder, high in purity and free from algal fishy smell, and can serve as various food raw materials.

Description

technical field [0001] The invention belongs to the technical field of marine biology application, and in particular relates to a preparation method and application of extracting phycoerythrin, polysaccharide and dietary fiber from seaweed. Background technique [0002] Seaweed is a large seaweed that is second only to kelp in China's cultivation. It is rich in nutrients and delicious in taste. The dried product after drying and processing has become a widely used food material in China. Seaweed is rich in polysaccharides, proteins, vitamins, and trace elements, which have the effects of improving human resistance, lowering blood sugar and blood lipids, and anti-aging. More and more people fall in love with eating seaweed, which makes seaweed have great application potential in the food industry. However, due to the eating habits of our country, there are not many varieties of seaweed products on the market, mainly laver cakes, instant laver sheets and small package soups, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L2/39A23L2/52A23L7/10A23L5/43A23L33/105A23L33/125A23L33/21A23L17/60A23L5/20C08B37/00A61K8/64A61Q19/00
CPCA23L2/39A23L2/52A23L7/115A23L5/43A23L33/105A23L33/125A23L33/21A23L17/60A23L5/28C08B37/0003A61K8/64A61Q19/00A23V2002/00A23V2400/249A23V2250/202A23V2250/51A23V2250/76A23V2300/44Y02P20/54
Inventor 张忠山王晓梅闻正顺毛根祥韩建芳张太生
Owner HUZHOU TEACHERS COLLEGE
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