Application of salvianolic acid C in preparing anti-stroke drug

A salvianolic acid and drug technology, applied in the field of medicine, can solve problems such as no report on the effect of salvianolic acid C, no report on the pharmacological effect of salvianolic acid C against stroke, etc., so as to reduce the apoptosis of nerve cells and improve the Symptoms of behavioral deficits and the effect of improving nerve cell viability

Inactive Publication Date: 2019-07-09
INST OF MATERIA MEDICA CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] So far, there is no report about the anti-stroke pharmacological effect of salvianolic acid C, and there is no report about the effect of salvianolic acid C in the preparation of anti-stroke drugs and / or health products

Method used

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  • Application of salvianolic acid C in preparing anti-stroke drug
  • Application of salvianolic acid C in preparing anti-stroke drug
  • Application of salvianolic acid C in preparing anti-stroke drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1: Effect of salvianolic acid C on neurobehavioral deficit symptoms in rats with cerebral ischemia-reperfusion

[0041] Experimental materials: SPF grade male SD rats, weighing 240-260 grams, purchased from Beijing Weitong Lihua Animal Technology Co., Ltd., certificate number: SCXK (Beijing) 2014-0004. Salvianolic acid C was prepared by the Institute of Materia Medica, Chinese Academy of Medical Sciences. Nimodipine was obtained from the Crystal Center of the Institute of Materia Medica, Chinese Academy of Medical Sciences.

[0042] Experimental grouping: SD rats were randomly divided into sham-operated control group, model group, low-dose salvianolic acid C group (1mg / kg), middle-dose group (3mg / kg), high-dose group (10mg / kg), Nemo Dipine group (20 mg / kg, positive control), 12 rats in each group.

[0043] Establishment of cerebral ischemia-reperfusion animal model: The middle cerebral artery occlusion / reperfusion (MCAO / R) model was prepared by suture emboliza...

Embodiment 2

[0046] Example 2: Effect of Salvianolic Acid C on Cerebral Infarct Volume in Rats with Cerebral Ischemia-Reperfusion

[0047] Experimental materials: TTC (2,3,5-triphenyltetrazolium chloride) was purchased from Sigma-Aldrich. Other relevant experimental materials are the same as in Example 1.

[0048] Experimental grouping: SD rats were randomly divided into sham operation control group, model group, salvianolic acid C low, medium and high dose groups, nimodipine group (20 mg / kg, positive control), 6 rats in each group. Other related experimental schemes are the same as in Example 1.

[0049] Cerebral infarction volume measurement: Put the rat brain tissue in a -20°C refrigerator, take it out after 15 minutes and place it in a rat brain slice mold, remove the olfactory bulb, cerebellum and lower brainstem, and slice coronally at intervals of 2mm. Then the brain slices were quickly placed in 0.5% TTC solution and incubated at 37°C in the dark for 20min. After TTC staining, t...

Embodiment 3

[0051] Example 3: Effect of salvianolic acid C on the percentage of cerebral edema in rats with cerebral ischemia-reperfusion

[0052] Experimental grouping: SD rats were randomly divided into sham operation control group, model group, salvianolic acid C low, medium and high dose groups, nimodipine group (20 mg / kg, positive control), 6 rats in each group. Relevant experimental materials and experimental scheme are the same as in Example 1.

[0053]Cerebral edema detection: After the rat brain tissue was taken out, the wet weight was weighed, and then the brain tissue was placed in a constant temperature drying oven at 100°C, and dried for 24 hours to a constant weight, which was the dry weight. Cerebral edema percentage=(wet weight-dry weight) / wet weight×100%.

[0054] Result: if image 3 As shown, the percentage of cerebral edema in the model group rats was 19.35±1.95%. The percentage of cerebral edema in the salvianolic acid C low-dose group (1 mg / kg) rats was 15.79±2.12%...

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Abstract

The invention discloses application of salvianolic acid C in preparing ischemic stroke resistant drug and / or healthcare products. The invention finds that the salvianolic acid C can remarkably improveneurobehavioral defect symptoms of cerebral ischemia-reperfusion rats, lower cerebral infarction volume and cerebral edema of the cerebral ischemia-reperfusion rats and reduce opening degree and permeability of rat blood brain barrier. The salvianolic acid C can be used for preparing the drug and / or the healthcare products for treating ischemic stroke, brain tissue nerve cell apoptosis can be reduced, survival rate of nerve cells can be increased, brain tissue injury degree can be lowered, and an effective solution is provided for disease prevention and treatment.

Description

technical field [0001] The invention relates to a new application of salvianolic acid C in the preparation of medicines, mainly relates to the application of salvianolic acid C in the preparation of anti-ischemic stroke drugs and / or health care products, and belongs to the technical field of medicine. Background technique [0002] Stroke is characterized by high morbidity, disability, and mortality, and is a global disease that seriously threatens human health. The types of stroke are mainly divided into ischemic stroke, hemorrhagic stroke and transient ischemic attack. Ischemic stroke is mainly caused by insufficient blood supply to brain tissue due to vascular embolism and / or arteriosclerosis, resulting in damage to brain tissue. Hemorrhagic stroke is mainly due to the rupture of blood vessels in the brain, and blood seeps into the brain, resulting in interruption of blood flow to normal brain tissue, resulting in damage to brain tissue. Ischemic stroke accounts for 70-8...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/343A61P9/10
CPCA61K31/343
Inventor 杜冠华宋俊科张雯吕扬杨海光周启蒙
Owner INST OF MATERIA MEDICA CHINESE ACAD OF MEDICAL SCI
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