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Electrochemical sensor of resixanthin d based on nano-cuo/nafion membrane-modified electrode

A technology for modifying electrodes and electrochemistry. It is applied in the fields of electrochemical variables of materials, scientific instruments, and instruments. It can solve the problems of time-consuming and expensive instruments, and achieve the effects of accurate methods, rapid detection and good application prospects.

Active Publication Date: 2021-09-24
FUJIAN UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the determination method of rehmanthin D is mainly high-performance liquid chromatography, and the instrument required by this method is more expensive and time-consuming. Therefore, it is more important to establish a simple, sensitive and rapid analytical method for rehmanthin D. value
[0004] At present, there is no electrochemical method applied to the analysis and detection of rehmanthin D

Method used

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  • Electrochemical sensor of resixanthin d based on nano-cuo/nafion membrane-modified electrode
  • Electrochemical sensor of resixanthin d based on nano-cuo/nafion membrane-modified electrode
  • Electrochemical sensor of resixanthin d based on nano-cuo/nafion membrane-modified electrode

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Example 1 Preparation of nano-CuO / Nafion membrane modified electrode of the present invention

[0058] a. Preparation of electrode modification solution: Dissolve 1 mg of nano-copper oxide powder in 1 mL of ethanol containing 20 μL of Nafion, and ultrasonically disperse for 2 hours to obtain a stable and well-dispersed black suspension;

[0059] b. Pretreatment of gold electrodes: Before the gold electrodes are modified, use 1.0, 0.3, 0.05μm α-Al in sequence 2 o 3 The powder is polished to a mirror surface on a professional polishing cloth, and after each polishing, it is cleaned by ultrasonic method in distilled water, and the treated electrode is placed in a solution containing 5mmol / L K 3 Fe(CN) 6 In the 0.5mol / L KCl solution, connect the three-electrode system, scan with cyclic voltammetry (CV method) at the scanning voltage -1V ~ +0.8V, and judge the degree of electrode polishing according to the redox peak potential difference. Activation is performed when the ...

Embodiment 2

[0061] Example 2 The electrochemical sensor based on the nano-CuO / Nafion membrane modified electrode of the present invention is used for the detection of rehmanniaside D in the rehmannia medicinal material

[0062] 1) Preparation of reference substance solution: Accurately weigh rehmanthin D reference substance, dissolve it with 10% acetonitrile to make a reference substance stock solution with a concentration of 1.0 mg / mL, store it at 4°C, and dilute it to 0.05 with supporting electrolytes during detection, 0.5, 1, 2, 5, 10, 20μg / mL;

[0063] 2) Preparation of the test solution: take 0.5g of Rehmannia glutinosa powder (passed through a 80 mesh sieve), weigh it accurately, place it in a stoppered Erlenmeyer flask, add 50mL of 60% methanol, soak for half an hour, 40kHz, 500W conditions Sonicate for 40 minutes, shake well, and filter. Evaporate the filtrate to dryness, dilute the residue in a 5mL volumetric flask with 10% acetonitrile, and set aside;

[0064] 3) Add the refer...

Embodiment 3

[0065] Example 3 The electrochemical sensor based on nano-CuO / Nafion membrane modified electrode of the present invention is used for the identification of Rehmannia glutinosa and Cynomorium cynomorium

[0066] 1) Preparation of reference substance solution: Accurately weigh rehmanthin D reference substance, dissolve it with 10% acetonitrile to make a reference substance stock solution with a concentration of 1.0 mg / mL, store at 4°C, and dilute to 20 μg / mL with supporting electrolyte during detection ;

[0067] 2) Preparation of the test solution: take 0.5 g of the sample powder to be tested (passed through a 80-mesh sieve), accurately weighed, place in a stoppered Erlenmeyer flask, add 50 mL of 60% methanol, soak for half an hour, 40 kHz, Sonicate at 500W for 40 minutes, shake well, and filter. Evaporate the filtrate to dryness, dilute the residue in a 5mL volumetric flask with 10% acetonitrile, and set aside;

[0068] 3) Add the reference substance solution and the test sa...

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Abstract

The invention provides a nano-CuO / Nafion membrane modified electrode, which uses a suspension composed of nano-CuO and Nafion ethanol solution as a modification liquid, which is drip-coated on the α-Al 2 o 3 The modified electrode is prepared by powder grinding and polishing, the surface of gold electrode activated by dilute sulfuric acid solution, and evaporated to dryness. The modified electrode of the present invention is simple to manufacture, and the rehmanthin D electrochemical sensor based on the nano-CuO / Nafion film modified electrode can be used for the detection of rehmanthin D in rehmannia medicinal material, and can also be used for the rapid identification of rehmannia cynomorium medicinal material. , and has good repeatability and rapid detection during detection. Tests have proved that the method for detecting rehmanniaside D in rehmannia glutinosa using CuO / Nafion membrane-modified electrodes has a recovery rate of 97.71%-106.5%.

Description

technical field [0001] The invention specifically relates to an electrochemical sensor based on nano-CuO / Nafion film modified electrodes, a preparation method and an application thereof. Background technique [0002] Chemically modified electrodes are electrodes made of conductors or semiconductors. Chemical or physical methods are used to design according to people's intentions to modify the surface of the electrodes to form functional films, such as accelerating electron transfer reactions, selective membrane permeation, and preferential enrichment. role, higher selectivity, sensitivity and stability. The design of the modification method, the rationality of the operation steps, and the degree of pros and cons directly affect the activity, stability and reproducibility of the chemically modified electrode, which is the basis for the research and application of the chemically modified electrode. [0003] Rehmannia glutinosa Libosch. Rehmannia glutinosa Libosch. is a perenn...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/333G01N27/48
CPCG01N27/333G01N27/48
Inventor 余丽双孙照霞周鑫悦张诗琪许惠凤褚克丹徐伟林羽
Owner FUJIAN UNIV OF TRADITIONAL CHINESE MEDICINE