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A method for intercropping enhanced extraction and remediation of mildly polluted farmland in cadmium

A farmland, light technology, applied in the field of soil heavy metal pollution phytoremediation, can solve problems such as difficulty in large-scale planting, and achieve the effect of improving modification effect, increasing absorption, and good yield level

Active Publication Date: 2021-03-30
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, Indian mustard has strong regional characteristics, and it is difficult to plant it in a large area in my country; it has been found from long-term research that many species or varieties of Brassica, where Indian mustard is located, have the above-mentioned characteristics

Method used

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  • A method for intercropping enhanced extraction and remediation of mildly polluted farmland in cadmium
  • A method for intercropping enhanced extraction and remediation of mildly polluted farmland in cadmium
  • A method for intercropping enhanced extraction and remediation of mildly polluted farmland in cadmium

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Experimental program
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preparation example Construction

[0036] The preparation method of the endophyte I fermentation liquid is as follows: inoculate the endophyte I to be tested in a sterile LB liquid medium, cultivate it at 30°C and 200 rpm in a fermenter for 24 hours, and then dilute it with sterile water Fermentation broth to OD value of about 1.0, made into a concentration of 1 × 10 8 CFU / mL of bacterial suspension is ready for use. The components of the medium are: tryptone 10g / L, yeast extract 5g / L, sodium chloride 10g / L, pH 7.2, sterilized at 121°C for 20min.

[0037] The preparation method of the endophyte II fermentation liquid is as follows: inoculate the endophyte II to be tested in a sterile LB liquid medium, cultivate it at 30°C and 200 rpm in a fermenter for 24 hours, and then dilute it with sterile water Fermentation broth to OD value of about 1.0, made into a concentration of 1 × 10 8 CFU / mL of bacterial suspension is ready for use. The components of the medium are: tryptone 10g / L, yeast extract 5g / L, sodium chl...

Embodiment 1

[0039] 1. Test location and soil physical and chemical properties

[0040] A heavy metal-contaminated farmland in Changxing County, Huzhou City (test point A), a heavy metal-contaminated farmland in Qujiang District, Quzhou City (test point B), and a heavy metal-contaminated farmland in Ninghai County, Ningbo City (test point C) were used as test fields.

[0041] The basic physical and chemical properties and heavy metal content of the soil at each test site are shown in Table 1.

[0042] Table 1 The basic physical and chemical properties of the soil at each test point

[0043]

[0044]

[0045] 2. Test steps

[0046] (1) At the end of September of the first year, seeds of rapeseed (Lvsheng No. 1) were sown in the field next to the soil to be repaired, and seedlings were raised; in October of the first year, the herbicide glyphosate was sprayed on the soil to be repaired , and then carry out normal plowing, soil preparation, and furrows; when the rapeseed seedlings grow ...

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Abstract

The invention discloses a method for enhanced extraction and repair of moderately cadmium contaminated farmland through intercropping of oilseed rape and sedum alfredii. The method includes transplanting the oilseed rape subjected to seedling culture into to-be-repaired soil, and in the bolting and flowering stages of the oilseed rape, applying heavy metal activators to the soil in a root region of the oilseed rape; transplanting seedlings of the sedum alfredii into to-be-repaired soil of the moderately cadmium contaminated farmland, and performing intercropping cultivation management; in theseedling and rapid growth stages of the sedum alfredii, spraying enhanced repairing agents onto leaves of the sedum alfredii; and harvesting oilseed rape plants and sedum alfredii plants. The method has the advantages that the oilseed rape and the sedum alfredii are intercropped, the heavy metal activators and the enhanced repairing agents are applied cooperatively, the absorption of cadmium in the to-be-repaired soil by the oilseed rape and the sedum alfredii is remarkably improved, and the soil repairing effect on the moderately cadmium contaminated farmland is further improved.

Description

technical field [0001] The invention relates to the technical field of phytoremediation of heavy metal pollution in soil, in particular to a method for enhancing the extraction and restoration of mildly polluted farmland in cadmium by intercropping rapeseed-sedum sedum. Background technique [0002] Phytoremediation is a method with unique advantages in technology and economy, and it is a promising remediation technology to solve the problem of heavy metal pollution in soil. However, the repair technology also has certain limitations, such as long repair period and slow effect. At this stage, there are mainly three ways to improve its purification efficiency of polluted soil: one is to screen ideal crops with high cadmium enrichment; the other is to use modern molecular engineering technology to reconstruct plants to improve their accumulation and tolerance of heavy metals in soil The third is to add growth-promoting bacteria or other strengthening measures to enhance the a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20B09C1/00
CPCB09C1/105
Inventor 杨肖娥陆敏曹雪蕊王小子杨倩颖
Owner ZHEJIANG UNIV
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