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A detection method based on polymer hindered sensitive membrane chronopotentiometry

A chronopotential and detection method technology, which is applied in measuring devices, material analysis through electromagnetic means, instruments, etc., can solve the problems of hindering sensitive membrane chronopotential, small ion flux, low sensitivity, etc., achieve high sensitivity and improve sensitivity , Versatile effect

Active Publication Date: 2021-04-16
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to overcome the disadvantages of low ion flux and low sensitivity of potentiometric sensors under traditional electrode conditions, and provide a detection method based on polymer barrier sensitive membrane chronopotential

Method used

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  • A detection method based on polymer hindered sensitive membrane chronopotentiometry
  • A detection method based on polymer hindered sensitive membrane chronopotentiometry
  • A detection method based on polymer hindered sensitive membrane chronopotentiometry

Examples

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Embodiment 1

[0045] HRP not only catalyzes the polymerization of dopamine, but also acts as a polydopamine deposition anchor point to form a dense polydopamine film on the surface of the sensitive membrane, which effectively hinders the ion flux through the sensitive membrane phase under chronopotential conditions, thereby reducing the potential signal and improving the potential of the potentiometric sensor. sensitivity.

[0046] (1) Preparation of polymer membrane calcium ion selective electrode sensitive membrane: weigh 60 mg polyvinyl chloride, 120 mg o-nitrophenyloctyl ether, 20 mg inert lipophilic salt tetrakis(dodecyl)-tetrakis(4 -Chlorophenyl)ammonium borate (ETH 500) and 1.84mg of calcium ionophore II (ETH 129) were dissolved in 2 mL of tetrahydrofuran and stirred evenly to obtain a calcium ionomer sensitive membrane solution. The potential conducting layer is poly(3,4-ethylenedioxythiophene)-polystyrenesulfonic acid; poly(3,4-ethylenedioxythiophene)-polystyrenesulfonic acid solut...

Embodiment 2

[0051] Example 2 Detection of target object oxytetracycline

[0052] HRP and Linker DNA molecules are loaded on the carboxylated multi-walled carbon nanotube matrix to form a recognition probe with catalytic and bonding functions, and the nucleic acid aptamer of the target to be detected is used as the recognition molecule. The probe is combined with the nucleic acid aptamer on the magnetic bead by DNA hybridization, and the recognition molecule and the probe are immobilized on the magnetic bead. When no target is present, the probe is bound to the surface of the magnetic bead, and the dopamine and H 2 o 2Add it to the alkaline magnetic bead solution, and use a pipette to take an appropriate amount and drop it on the surface of the polymer sensitive membrane to react for a certain period of time. The HRP molecule on the probe can effectively catalyze the polymerization of dopamine on the surface of the polymer sensitive membrane to form a dense polydopamine film, which hinde...

Embodiment 3

[0062] Embodiment 3: Detection of target DNA

[0063] Loading HRP and Linker DNA molecules on the carboxylated multi-walled carbon nanotube matrix to form a recognition probe with catalytic and bonding functions to Escherichia coli ( E. coli -DNA) as the target. The target can be combined with the probe and the nucleotides on the magnetic beads through DNA hybridization to form a magnetic bead-target-probe complex. Combine dopamine and H 2 o 2 Add it to the alkaline magnetic bead solution, take a proper amount of it with a pipette and drop it on the surface of the polymer sensitive membrane to react for a certain period of time. The HRP molecules on the probe can effectively catalyze the polymerization of dopamine on the surface of the polymer sensitive membrane to form a dense layer of polymer Dopamine film, which blocks ion flux through the sensitive membrane phase under chronopotentiometry conditions, reduces the potentiometric signal. When no target is present, the pr...

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Abstract

The invention relates to a barrier-based sensitive membrane potential analysis technology, in particular to a polymer barrier-based sensitive membrane chronopotential detection method. The polymer membrane ion-selective electrode is used as the working electrode, and the nucleic acid aptamer, DNA or antigen / antibody of the target to be detected is used as the recognition molecule, and the enzyme or its analog molecule is used as the recognition probe by loading or labeling the probe substrate , the chronopotential technology controlled by pulse constant current is used to carry out quantitative / qualitative detection of the target in the liquid to be detected; the method of the present invention has certain versatility, and can be realized by changing the aptamer sequence of the recognition molecule or using DNA, antigen / antibody Detection of different targets. In the detection process, a sensitive membrane containing an ion exchanger can also be used to detect the target object by measuring the open circuit potential of the electrode.

Description

technical field [0001] The invention relates to a barrier-based sensitive membrane potential analysis technology, in particular to a polymer barrier-based sensitive membrane chronopotential detection method. Background technique [0002] Potentiometric sensors based on polymer membrane ion-selective electrodes are an important branch of the field of electrochemical sensors. The change of ion flux through the polymer-sensitive membrane phase plays an important role in studying the ion conduction process at the liquid / liquid interface. In recent years, potentiometric sensors based on the barrier effect have aroused great interest of experts and scholars. However, the existing barrier-based potentiometric sensors are not sensitive, mainly due to the difficulty in obtaining effective barriers to sensitive membrane interfaces. In addition, under the traditional electrode conditions, the ion flux through the polymer sensitive membrane phase is small, and the potential response i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327G01N27/333
CPCG01N27/3271G01N27/3275G01N27/333
Inventor 丁家旺刘淑文秦伟
Owner YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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