Method for preparing insoluble dietary fibers by extrusion and enzymolysis of pea residues
A technology of soluble dietary fiber and pea dregs, applied in the field of food processing, can solve the problems of inconvenient transportation of slurry, low purity of dietary fiber, poor storage stability, etc., achieve low production input cost, improve expansibility, adsorption performance and expansion And the effect that the water holding performance is high
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Embodiment 1
[0029] (1) Mix 7kg of pea dregs with 3kg of water, then extrude with a single-screw extruder to obtain pea dregs extrudates; the operating parameters of the single-screw extruder are: sleeve temperature 110°C, screw speed 120r / min , the diameter of the die hole is 8mm;
[0030] (2) After crushing the extrudate of pea dregs, add 140kg of water, first use 5wt% NaOH to adjust the pH of the mixed solution to 5.8, add 3.5g of high-temperature-resistant α-amylase, and hydrolyze it for 40min at 90°C. turn blue;
[0031] Then adjust the temperature to 55°C, adjust the pH of the mixture to 3.0 with 5wt% HCL, add 35g of glucoamylase, and hydrolyze at 55°C for 5h;
[0032] Finally, adjust the temperature to 50°C, adjust the pH of the mixture to 7.8 with 5wt% NaOH, add 14g of alkaline protease, and hydrolyze at 50°C for 5 hours to obtain a hydrolysis mixture;
[0033] (3) Incubate the hydrolysis mixture obtained in step (2) for 10 minutes at 100° C., perform enzyme inactivation treatmen...
Embodiment 2
[0037] (1) Mix 5.5kg of pea dregs with 4.5kg of water, then extrude with a single-screw extruder to obtain pea dregs extrudates; the working parameters of the single-screw extruder are: sleeve temperature 160°C, screw speed 200r / min, die hole diameter 12mm;
[0038](2) After crushing the pea dregs extrudate, add 170kg of water, adjust the pH of the mixed solution to 7.8 with 5wt% NaOH, add 1.4g of high-temperature-resistant α-amylase, and hydrolyze it at 95°C for 30min. turn blue;
[0039] Then adjust the temperature to 65°C, adjust the pH of the mixture to 5.5 with 5wt% HCL, add 5.5g of glucoamylase, and hydrolyze at 65°C for 3 hours;
[0040] Finally, adjust the temperature to 60°C, adjust the pH of the mixture to 8.5 with 5wt% NaOH, add 5.5g of alkaline protease, and hydrolyze at 60°C for 3 hours to obtain a hydrolysis mixture;
[0041] (3) Incubate the hydrolysis mixture obtained in step (2) for 10 minutes at 100° C., perform enzyme inactivation treatment, then centrifu...
Embodiment 3
[0045] (1) Mix 6kg of pea dregs with 4kg of water, then extrude with a single-screw extruder to obtain pea dregs extrudates; the operating parameters of the single-screw extruder are: sleeve temperature 140°C, screw speed 180r / min , the diameter of the die hole is 10mm;
[0046] (2) After crushing the pea dregs extrudate, add 180kg of water, first use 5wt% NaOH to adjust the pH of the mixed solution to 6.8, add 2g of high-temperature-resistant α-amylase, and hydrolyze it at 92°C for 40min, and the iodine solution does not change blue;
[0047] Then adjust the temperature to 60°C, adjust the pH of the mixture to 4.5 with 5wt% HCL, add 12g of glucoamylase, and hydrolyze at 60°C for 4 hours;
[0048] Finally, adjust the temperature to 55°C, adjust the pH of the mixture to 8.0 with 5wt% NaOH, add 8g of alkaline protease, and hydrolyze at 55°C for 4 hours to obtain a hydrolysis mixture;
[0049] (3) Incubate the hydrolysis mixture obtained in step (2) for 10 minutes at 100° C., p...
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