Check patentability & draft patents in minutes with Patsnap Eureka AI!

Method for detecting cell membrane protein overexpression based on nano fluorescent probe and application thereof

A nano-fluorescent probe, cell membrane technology, applied in nano-optics, nano-technology, nano-technology and other directions, can solve problems such as high cost and dependence on large-scale instruments, and achieve the effect of easy operation, easy promotion, and stable effect.

Active Publication Date: 2019-08-06
LIAONING UNIVERSITY
View PDF5 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above detection methods have certain limitations and problems, such as dependence on large instruments, high cost, timeliness, etc.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for detecting cell membrane protein overexpression based on nano fluorescent probe and application thereof
  • Method for detecting cell membrane protein overexpression based on nano fluorescent probe and application thereof
  • Method for detecting cell membrane protein overexpression based on nano fluorescent probe and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A method for detecting cell membrane protein overexpression based on nano fluorescent probes, comprising the steps of:

[0029] (1) Preparation of nano-fluorescent probes

[0030] Weigh 2g (13mmol) histidine and 0.024g valine (2mmol), dissolve them in 20mL ultrapure water, add 0.18g (3mmoL) ethylenediamine, after complete ultrasonic dissolution, in the reaction kettle at 210°C Heated for 15 hours to obtain nanometer fluorescent probes.

[0031] figure 1 is the scanning electron microscope image of the prepared nano-fluorescence probe, from figure 1 It can be seen that the average particle size of the prepared nano fluorescent probe is about 4nm, and the distribution is uniform, forming uniformly distributed spherical particles.

[0032] Figure 2A It is the fluorescence spectrum diagram obtained by using the fluorescence spectrophotometer to detect the fluorescence at the optimal excitation wavelength for the prepared nano fluorescent probe sample. Depend on Figu...

Embodiment 2

[0040] A method for screening drugs that inhibit the overexpression of cell membrane proteins, the method is as follows:

[0041] 1. Use a cell scraper to separate the overexpressed microglial cells from the culture flask, and plant them in 26 6-well plates so that the number of cells is 5×10 5 indivual. After 4 hours of cell attachment, 23 kinds of drugs with a concentration of 5 μg / ml, such as sulindac and metformin hydrochloride, were added, and placed in 5% CO 2 Cultivate in the incubator for 24h. After the cells were taken out and washed three times with cold PBS, the cells were separated from the 6-well plate with a cell scraper, then centrifuged at 1000r / min, 4°C for 4min, redispersed in 1ml of 10mmol / LTris-HCl buffer solution, and used Under the condition of 300w, the cell disruptor ultrasonicated for 3s with an interval of 7s for 3min to disrupt the cells. According to the differential centrifugation method, it is placed in a low-temperature centrifuge for the sepa...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
The average particle sizeaaaaaaaaaa
Login to View More

Abstract

The invention relates to a method for detecting cell membrane protein overexpression based on a nano fluorescent probe and application thereof. A cell membrane protein solution and a nano-fluorescentprobe solution are added into a 96 pore plate; incubation is performed for 5 to 10 minutes at a temperature of 20 to 25; and then the fluorescence intensity is detected. The preparation method for thenano-fluorescent probe solution includes: dissolving a carbon source completely by water; adding a small molecule ligand into the solution and carrying out doping; after ultrasonic dissolving, carrying out heating reaction at a temperature of 190 to 220 DEG C to obtain a nano fluorescent probe; carrying out filtering, dialysis and freeze-drying on the nano fluorescent probe; and then dispersing the processed probe into a PBS buffer solution to obtain a nano-fluorescent probe solution. With the provided method, cell membrane proteins with different response states can be identified. The methodhas characteristics of high biocompatibility, high safety, non-toxicity and stable action effect. The overexpression of the cell membrane protein can be identified specifically. The preparation method has advantages of having simple processes, being easy to operate, requiring low preparation cost and being easy to promote.

Description

technical field [0001] The invention belongs to the technical field of fluorescent nanometer materials, and in particular relates to a method for detecting overexpression of cell membrane proteins based on nanometer fluorescent probes and an application thereof. Background technique [0002] Protein is the material basis of life, an organic macromolecule, the basic organic matter that constitutes a cell, and the main bearer of life activities. Without protein there is no life. Every cell and every major part of the body has proteins involved. Protein is an important component of body cells and the main raw material for human tissue renewal and repair. It mainly maintains the normal metabolism of the body and the transportation of various substances in the body. For example: Carrier proteins are crucial to maintaining normal life activities of the human body. Various substances can be carried in the body. For example, hemoglobin transports oxygen (renewal rate of red blo...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N21/64C09K11/06B82Y40/00B82Y20/00
CPCG01N21/6428C09K11/06B82Y20/00B82Y40/00
Inventor 刘学毕秀丽刘禹霖陈扬
Owner LIAONING UNIVERSITY
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com