Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of Liu-Shen-Wan in preparation of medicines for preventing and treating flu-type inflammatory diseases

A technology for inflammatory diseases and influenza, applied in the field of new uses of traditional Chinese medicine, can solve problems such as difficulty in inhibiting inflammatory signaling cascades

Active Publication Date: 2019-08-09
雷允上药业集团有限公司
View PDF2 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, it is very important to effectively control the excessive immune inflammatory response caused by influenza virus infection, but most of the current drug research focuses on the immune regulation of a single target, and it is always difficult to inhibit the complex inflammatory signal transduction cascade reaction. limitations in disease

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of Liu-Shen-Wan in preparation of medicines for preventing and treating flu-type inflammatory diseases
  • Application of Liu-Shen-Wan in preparation of medicines for preventing and treating flu-type inflammatory diseases
  • Application of Liu-Shen-Wan in preparation of medicines for preventing and treating flu-type inflammatory diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Toxicity evaluation experiment of Liushen Pill.

[0030] 1. Method.

[0031] Dissolve Liushen Wan powder in DMSO, after ultrasonic filtration, add culture medium to dilute to 5mg / mL, filter through a 0.22μm filter membrane and store at 4°C; 4 18-24 hours after the cells grow into a single layer, discard the culture medium, add 100 μL / well of drugs at different dilutions, add 100 μL / well of DMEM to the control wells of normal cells, and store at 37°C, 5% CO 2 Continue culturing for 48 hours, add 20 μL of MTT solution (5 mg / mL) to each well, and place at 37°C, 5% CO 2 Incubation was continued for 4 hours in the incubator. Discard the culture supernatant, add 100 μL dimethyl sulfoxide (DMSO) to each well, and shake at low speed for 10 minutes to fully dissolve the crystals. Select a wavelength of 490nm, and measure the light absorption value of each well on an enzyme-linked immunosorbent monitor.

[0032] Calculate the inhibition rate according to the following formul...

Embodiment 2

[0037] Influenza virus median lethal titer (TCID) 50 ) determination.

[0038] 1. Method.

[0039] Divide dog kidney cells (MDCK) into 3×10 4 cells / well, inoculated into 96-well plates, placed at 37°C, 5% CO 2Cultivate until the monolayer grows. Add 10-fold diluted influenza virus solution, 100 μL / well, and incubate at 37°C for 2 hours; discard the supernatant, add fresh culture medium, 100 μL / well, observe the results after 2 days, and record the CPE.

[0040] And use Reed-Muench to calculate the virus half lethal titer (TCID 50 ) [Refer to Reed LJ, Muench H. Asimple method of estimating fifty percent endpoints. Am J Hyg, 1938, 27(3):493-497].

[0041] 2. Results.

[0042] The median lethal titer of the amplified PR8 subtype influenza virus was detected to be 10 by Reed-Muench methodology 7.75 TCID 50 / 0.1mL.

Embodiment 3

[0044] Evaluation of Anti-influenza Virus Efficacy of Liushen Pill

[0045] 1. Method.

[0046] 96-well plate monolayer cells were washed once with PBS, added with about 100TCID 50 Virus dilution 100 μL / well, 37°C, 5% CO 2 Incubate in the incubator for 2 hours, discard the virus solution, add 2-fold gradient dilution of the drug, set 4 replicate wells for each concentration, take the maximum non-toxic concentration as the initial concentration of the drug, and incubate at 37°C, 5% CO 2 Incubate for 2 days in the incubator. Observe the cell lesion, stain with MTT, and measure the OD value.

[0047] The half effective drug concentration (IC) was calculated by the Reed-Muench method. 50 ), calculate the therapeutic index (SelectiveIndex, SI): SI=TC 50 / IC 50 ). [Refer to "Pharmacological Experimental Methodology, edited by Xu Shuyun", the therapeutic index (SI)>1 means effective].

[0048] 2. Results.

[0049] The drug efficacy of Liushen Pill was evaluated by MDCK cytop...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to application of Liu-Shen-Wan in preparation of medicines for preventing and treating flu-type inflammatory diseases, and belongs to the technical field of novel application oftraditional Chinese medicines. According to the application, it is discovered for the first time that the Liu-Shen-Wan has an antiviral effect on influenza viruses, and can regulate the immune function, exert the anti-inflammatory effect, block infection on host cells by the influenza viruses, inhibit the multi-target-spot intervention effect of host excessive inflammatory response induced by theinfluenza viruses and be used for disease treatment in the aspect of preventing and treating the inflammation infected by the influenza viruses.

Description

technical field [0001] The invention relates to the technical field of new uses of traditional Chinese medicines, in particular to the application of Liushen Pills in the preparation of medicines for preventing and treating influenza inflammatory diseases. Background technique [0002] Respiratory tract infectious inflammatory diseases rank first among acute infectious diseases in the world. 90% of them are caused by viral infection, and cause about 500,000 deaths per year (Clinics in chest medicine, 2017, 38(1):11-9). Although most respiratory bacterial infections can be effectively controlled by antibacterial drugs, however, the prevention and treatment methods for influenza virus infection are still stretched. Clinical classic drugs include M2 ​​ion channel inhibitors (such as amantadine) and neuraminidase inhibitors (such as oseltamivir), but due to strong virus variability and frequent drug resistance, the disease is not treated well In addition, the starting point of...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/65A61P29/00A61P31/16A61K35/618A61K35/413A61K35/55A61K33/36A61K31/045
CPCA61K35/618A61K35/413A61K35/55A61K33/36A61K35/65A61K31/045A61P29/00A61P31/16A61K2300/00Y02A50/30
Inventor 马钦海杨子峰王恒斌李全翟云良辛艳罗年翠戴晶晶
Owner 雷允上药业集团有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com