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Marine fungus-derived naphthoquinone compound and anti-inflammatory application thereof

A technology of marine fungi and compounds, applied in the field of medicinal compounds, can solve the problems of side effects, adverse reactions and tolerance, and achieve the effect of good application prospects and broad development space.

Active Publication Date: 2019-08-13
GUANGZHOU UNIVERSITY OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to overcome the shortcomings of existing anti-inflammatory drugs that have side effects, adverse reactions and tolerance to patients, and provide a marine fungus that can produce anti-inflammatory substances, and isolate a New anti-inflammatory drugs, specifically a brand-new naphthoquinone compound, which can inhibit LPS to stimulate macrophages to produce NO, play a significant anti-inflammatory effect, and have great market prospects in the preparation of anti-inflammatory drugs

Method used

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  • Marine fungus-derived naphthoquinone compound and anti-inflammatory application thereof
  • Marine fungus-derived naphthoquinone compound and anti-inflammatory application thereof
  • Marine fungus-derived naphthoquinone compound and anti-inflammatory application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1 The acquisition of marine fungus Diaporthe sp.GZU-1021

[0049] The inventor team isolated and screened a fungus (number: GZU-1021) from the torso of small animal sea crabs in Longxue Island, Nansha District, Guangzhou City, Guangdong Province, and identified the fungus by DNA amplification and sequencing of the ITS region of the fungus .

[0050] References for specific steps (Nature protocols, 2010, 5, 480-490; Journal of Natural Products, 2006, 69, 11, 1622-1625): Using the DNeasy Plant Mini Kit kit, according to the instructions, the DNA of the fungus was extracted, and the fungus Design primers for the ITS region for PCR amplification, and sequence the amplified product to obtain the nucleotide sequence of the ITS-rRNA gene of the fungus, as shown in SEQ ID NO.1; the sequence is searched and compared for similarity through the BLAST database After analysis and comparison, the similarity between the sequence and the sequence of the fungus Diaporthe sp. wa...

Embodiment 2

[0052] The separation and identification of embodiment 2 naphthoquinone compounds

[0053] 1. Isolation of Compound 1

[0054] The compound is isolated from the fermentation broth of the marine fungus Diaporthe sp.GZU-1021 obtained in Example 1, and the specific method is as follows:

[0055] S1. Seed liquid culture of fungus Diaporthe sp.GZU-1021: medium composition by weight: glucose 0.3%, yeast extract 0.1%, peptone 0.5%, agar 2.5%, sodium chloride 3%, water supplement 100% ; Make a test tube slope, pick the strains into the slope, and culture at 30°C for 6 days;

[0056] S2. Fermentation culture of the fungus Diaporthe sp.GZU-1021: Using a solid rice medium (rice: seawater = 1:1), transfer the strain obtained from the seed liquid culture in step S1 into a solid rice medium, and statically place it at room temperature at 35°C. Set 60d;

[0057] S3. extracting the product after fermentation and cultivation in step S2 with methanol for 3 times, concentrating the extract, and...

Embodiment 3

[0067] Example 3 Anti-inflammatory Cell Screening Model and Cytotoxicity Screening Research of Naphthoquinone Compounds

[0068] Macrophages are an important type of immune cells in the body. They are the main cells that initiate the production of inflammatory mediators in the body, and have the functions of anti-infection, anti-tumor and immune regulation. At present, studies believe that macrophages and the inflammatory mediators they release are involved in the occurrence and development of various inflammatory diseases, such as atherosclerosis, rheumatoid arthritis, inflammatory bowel disease, sepsis, gouty arthritis, etc. In the development of anti-inflammatory drugs, macrophage RAW264.7 is an important screening model. When the exogenous antigen lipopolysaccharide LPS stimulates macrophages, a series of immune responses will be produced, and the inflammatory factor NO will be released at the same time. 2 - , NO 2 - The level of the level can indirectly reflect the de...

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Abstract

The invention discloses a marine fungus-derived naphthoquinone compound and anti-inflammatory application thereof. A marine fungus Diaporthe sp.GZU-1021 is obtained through separation and purification, and preserved in the Guangdong General Microbiological Culture Collection Center (GDMCC), and the preservation number is GDMCC No: 60601. The strain can secrete the brand-new naphthoquinone compound, and the compound has the effect of remarkably inhibiting generation of LPS-induced NO and has the clinical application potential for anti-inflammatory treatment. Therefore, the naphthoquinone compound has good application prospects and wide development space in preparation of anti-inflammatory drugs.

Description

technical field [0001] The invention belongs to the technical field of medical compounds, and more specifically relates to a marine fungal-derived naphthoquinone compound and its anti-inflammatory application. Background technique [0002] Inflammation is a defensive response of body tissues to damaging stimuli (such as harmful stimuli, germs or physical damage), and is also an important factor in the aging process of the human body. [0003] There are two main types of anti-inflammatory drugs commonly used in clinical practice: non-steroidal anti-inflammatory drugs and steroidal anti-inflammatory drugs. However, long-term and large-scale use will produce a series of adverse reactions, side effects and tolerance, such as gastric mucosal damage, liver damage, kidney damage, etc. Therefore, in order to solve drug tolerance and adverse reactions, finding new anti-inflammatory drugs has become a research hotspot. [0004] Natural products are an important source of drug develo...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P17/04C07D307/92A61P29/00C12R1/645
CPCC12P17/04C07D307/92A61P29/00C12R2001/645C12N1/145
Inventor 崔辉赵钟祥阮清锋江诗琴
Owner GUANGZHOU UNIVERSITY OF CHINESE MEDICINE
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