A Fluorescence Detection Method of Oxytetracycline Based on Surface Passivation and Covalent Coupling

A technology of covalent coupling and fluorescence detection, which is applied in the fields of fluorescence/phosphorescence, measurement devices, and material analysis through optical means, and can solve problems such as poor detection performance, low fluorescence recovery efficiency, and high background signal

Active Publication Date: 2021-09-24
DALIAN UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The invention solves the shortcomings of the existing oxytetracycline fluorescence sensing detection method, such as excessively high background signal, low fluorescence recovery efficiency and poor detection performance, and provides a simple, fast and highly sensitive method for detecting oxytetracycline in water

Method used

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  • A Fluorescence Detection Method of Oxytetracycline Based on Surface Passivation and Covalent Coupling
  • A Fluorescence Detection Method of Oxytetracycline Based on Surface Passivation and Covalent Coupling
  • A Fluorescence Detection Method of Oxytetracycline Based on Surface Passivation and Covalent Coupling

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Experimental program
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Effect test

Embodiment 1

[0028] Configure the determination of oxytetracycline content in the water sample:

[0029] (1) Preparation of metal-organic framework nanosheets (Cu(HBTC)-1):

[0030] Add copper chloride (CuCl 2 ) (2.5M) was added 0.1 g of polyvinylpyrrolidone (PVP, K30). After the mixture was stirred for 5min, 2.5mL of sodium hydroxide (0.2M) was slowly added dropwise, and after stirring for 10min, 2.5mL of freshly prepared ascorbic acid (0.1M) was slowly added dropwise, and the stirring was continued for 10min. Cubic cuprous oxide (Cu 2 O) Nanoparticles. Subsequently, the solid was collected by centrifugation under the condition of 8000 r / min, washed with ethanol for 3 times, and then dispersed into 10 mL of ethanol solution again. Then weigh 0.4g PVP and dissolve it in 60mL high-purity water, and add 4mL trimesic acid ethanol solution (0.24M). After the mixture was stirred for 10 min, the Cu prepared above was added 2 O ethanol dispersion, the mixture turned into a transparent color...

Embodiment 2

[0042] Determination of oxytetracycline content in tap water samples:

[0043] (1) Preparation of metal-organic framework nanosheets (Cu(HBTC)-1):

[0044] Add copper chloride (CuCl 2 ) (2.5M) was added 0.1 g of polyvinylpyrrolidone (PVP, K30). After the mixture was stirred for 5min, 2.5mL of sodium hydroxide (0.2M) was slowly added dropwise, and after stirring for 10min, 2.5mL of freshly prepared ascorbic acid (0.1M) was slowly added dropwise, and the stirring was continued for 10min. Cubic cuprous oxide (Cu 2 O) Nanoparticles. Subsequently, the solid was collected by centrifugation under the condition of 8000 r / min, washed with ethanol for 3 times, and then dispersed into 10 mL of ethanol solution again. Then weigh 0.4g PVP and dissolve it in 60mL high-purity water, and add 4mL trimesic acid ethanol solution (0.24M). After the mixture was stirred for 10 min, the Cu prepared above was added 2 O ethanol dispersion, the mixture turned into a transparent color within 2min....

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Abstract

The invention belongs to the technical field of environmental monitoring, and relates to an oxytetracycline fluorescence detection method based on surface passivation and covalent coupling. The covalent coupling between the surface of two-dimensional MOF nanosheets and DNA can effectively utilize the limited adsorption sites on the surface of MOF nanosheets, enhance the ability of nanosheets to adsorb DNA molecules, and reduce the background fluorescence signal. At the same time, the use of short-chain DNA molecules as signal reporter molecules can effectively improve the fluorescence recovery efficiency, while the addition of surface passivator sodium polystyrene sulfonate in the reaction system further weakens the adsorption ability of nanosheets to signal reporter molecules, making MOF nanosheets The chip sensor improves the linear response to low concentration OTC and enhances the detection performance of OTC. The detection method of the invention can effectively reduce the fluorescence background value, increase the signal response value induced by the target, and effectively improve the detection performance of the fluorescent sensing platform constructed based on the principle of physical adsorption for oxytetracycline.

Description

technical field [0001] The invention belongs to the technical field of environmental monitoring, and relates to an oxytetracycline fluorescence sensing and detection method based on surface passivation and DNA covalent coupling modification of metal organic framework (MOFs) nanosheets. Background technique [0002] Antibiotics are an important class of drugs for the prevention and treatment of bacterial infections, and are widely used in human medicine, poultry, livestock, and aquatic product farming. Among them, oxytetracycline (OTC), as a broad-spectrum antibacterial tetracycline antibiotic, is widely used in the prevention and treatment of various bacterial infection diseases. Because antibiotics can only be partially metabolized in human or animal bodies, most antibiotics are excreted in the form of their parent structure through urine or feces, and enter water or soil. In addition to causing harm to the ecological environment, antibiotics entering the environmental med...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
CPCG01N21/6402
Inventor 赵慧敏谭冰
Owner DALIAN UNIV OF TECH
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