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A method for producing bacterial cellulose by substrate circulation and continuous fermentation

A technology of bacterial cellulose membrane and cellulose membrane, which is applied in the field of continuous fermentation of substrates to produce bacterial cellulose, can solve problems such as unfavorable yield, influence of nutrient transfer, decrease of synthesis rate, etc., and achieve shortened production cycle, stable density, and improved production Efficiency-enhancing effect

Active Publication Date: 2021-03-30
JIANGNAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the prolongation of fermentation time and the increase of film thickness, the transfer of nutrients is affected, and the synthesis rate of BC decreases, which is not conducive to the continuous increase of yield

Method used

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  • A method for producing bacterial cellulose by substrate circulation and continuous fermentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Spread the bacterial strain Komagataeibacter rhaeticus WM407-1 (CCTCC NO: M2016213) stored in glycerol tubes on a solid plate and activate it for 1 day, then pick it and insert it into the seed medium, at 150-200r / min, 30°C Cultivate for 2 days, shaking or stirring for 5 minutes every 4 hours. Then the above-mentioned seed liquid is inserted into the fermentation medium under the Erlenmeyer flask or the fermenter system with the inoculum amount of 6% by volume fraction, and it is shaken intermittently or stirred for 1 day at 30°C (every 4h shakes or stirs 5min, and the shaking speed is 150 -200r / min or the stirring speed is 50-100r / min). Transfer all the fermented liquid cultivated for 1 day into a sterilized stainless steel drum with a movable screen (volume 1L, liquid capacity 600mL), and continue to culture at 30°C. After 2 days of culture, the gas-liquid interface Form a layer of cellulose film, press the BC film grown on the air-liquid interface into the liquid su...

Embodiment 2

[0032]Spread the bacterial strain Komagataeibacter rhaeticus WM407-1 (CCTCC NO: M2016213) stored in glycerol tubes on a solid plate and activate it for 1 day, then pick it and insert it into the seed medium, at 150-200r / min, 30°C Cultured with shaking for 2 days. Then the above-mentioned seed liquid is inserted into the fermentation medium under the Erlenmeyer flask or fermenter system according to the inoculum amount of 6% by volume fraction, and it is intermittently shaken or stirred for 1 day at 30°C, and shakes or stirs for 5min every 4h, and the shaking speed is 150-200r / min or the stirring speed is 50-100r / min. Transfer all the fermented liquid cultured for 1 day into a sterilized stainless steel drum A with a movable screen (volume 1L, liquid capacity 600mL), and continue the static culture at 30°C. After 2 days of culture, put The mobile screen is taken out, and the generated bacterial cellulose film is put into the sterilized stainless steel drum B, and the screen of...

Embodiment 3

[0034] Spread the bacterial strain Komagataeibacter rhaeticus WM407-1 (CCTCC NO: M2016213) stored in glycerol tubes on a solid plate and activate it for 1 day, then pick it and insert it into the seed medium, at 150-200r / min, 30°C Cultivate for 2 days, shaking or stirring for 5 minutes every 4 hours. Then the above-mentioned seed liquid is inserted into the fermentation medium under the Erlenmeyer flask or the fermenter system with the inoculum amount of 6% by volume fraction, and it is shaken intermittently or stirred for 1 day at 30°C (every 4h shakes or stirs 5min, and the shaking speed is 150 -200r / min or the stirring speed is 50-100r / min). Transfer all the fermented liquid cultivated for 1 day into stainless steel drum A with a movable screen after disinfection (volume 1L, liquid capacity 600mL), and continue static cultivation at 30°C. After 2 days of cultivation, the gas-liquid A layer of cellulose film is formed on the interface, and the BC film grown on the air-liqui...

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Abstract

The present invention discloses a method for producing bacterial cellulose by a matrix circulation continuous fermentation, belongs to the technical field of fermentation engineering and provides themethod for producing the bacterial cellulose by the continuous fermentation. In a process of producing the bacterial cellulose by the K.rhaeticus, a BC membrane-moving away, bacterium and culture medium replacement, and material supplement operation mode is used to improve production efficiency of the bacterial cellulose, at the same time, costs of the culture mediums are also further reduced, andthe method has a relatively good application prospect in production industry of the bacterial cellulose.

Description

technical field [0001] The invention relates to a method for producing bacterial cellulose by substrate circulation and continuous fermentation, and belongs to the technical field of fermentation engineering. Background technique [0002] Bacterial Cellulose (BC) is a kind of pure cellulose produced by microorganisms. It is a high molecular polymer composed of glucose monomer molecules polymerized by α-1,4-glycosidic bonds. Compared with plant fibers, BC does not contain lignin and hemicellulose, has excellent biocompatibility and biodegradability, and unique properties such as fine network structure, high crystallinity, and high tensile strength, and is recognized as It is a new biomaterial with excellent performance that is safe in the world. There are only a few species of bacteria that produce BC in nature, mainly concentrated in the genus Acetobacter and Pseudomonas. Among them, Acetobacter xylinus in Acetobacter is the earliest and most thoroughly studied BC-producing...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/04C12R1/01
CPCC12P19/04
Inventor 王淼康文术曾伟主颜少慰李萧
Owner JIANGNAN UNIV