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A method for enzymatic hydrolysis of concentrated mash from agricultural and forestry biomass to produce sugar

A technology of enzymatic hydrolysis, agriculture and forestry, applied in the field of enzyme engineering, can solve the problems of low concentration of fermentable sugar and low efficiency of cellulose enzymatic hydrolysis, and achieve the effects of low enzyme load, improved enzymatic hydrolysis efficiency and low cost

Active Publication Date: 2020-12-29
JIANGNAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the problems of low cellulose enzymatic hydrolysis efficiency and low concentration of fermentable sugar in the current enzymatic hydrolysis process, the purpose of the present invention is to provide a compound surfactant as an enzymatic hydrolysis aid to strengthen Method for producing sugar by enzymatic hydrolysis of concentrated mash from agricultural and forestry biomass

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Put the substrate pretreated with normal pressure glycerin organic solvent (industrial glycerin purchased from Wuxi Chemical Industry Station, purity ≥ 99.0%) into the pH4.8 citric acid buffer solution;

[0039] Add cellulase and compounded surfactant to (1); after enzymatic hydrolysis at 50° C. for 24-72 hours, obtain an enzymatic hydrolyzate containing reducing sugars and perform detection.

[0040] In the above steps, the substrate concentration is 15%-45%, the enzyme load is 0.1-3FPU / g, and the types and concentrations of the compounded surfactants are: 25mg / g Tween80, 8.5mg / g PEG4000, 8.5mg / g tea Saponin, 27.7 mg / g Triton 100 and 8.5 mg / g Lauryl Betaine. Xylanase 0.5-1.5 mg / g, β-xylosidase 0.5-1.5 mg / g, soluble polysaccharide monooxygenase 0.2-1 mg / g and bovine serum albumin 5-15 mg / g. Under these conditions, the fermentable sugar concentration reaches 150-230 g / L after 24-72 hours of enzymatic hydrolysis.

Embodiment 2

[0042] (1) Put the matrix pretreated by an atmospheric glycerol organic solvent (industrial glycerol was purchased from Wuxi Chemical Industry Station, with a purity ≥ 99.0%) in a pH 4.8 citric acid buffer solution;

[0043] (2) Add cellulase and compounded surfactant to (1); after enzymatic hydrolysis at 50° C. for 72 hours, obtain an enzymatic hydrolysis solution containing reducing sugars and perform detection.

[0044] Enzymolysis adopts the method of mechanical stirring and wet base (water content 50%) enzymolysis. The specific steps are as follows: the initial substrate concentration is 8%, and 4% is fed at 6h, 12h and 18h ​​respectively, so that the final concentration is 20%.

[0045] In the above steps, the substrate concentration is 20%, the enzyme load is 2FPU / g, and the types and concentrations of the compounded surfactants are: 25mg / g Tween80, 8.5mg / g PEG4000, 8.5mg / g tea saponin, 27.7mg / g triton 100 and 8.5mg / g lauryl betaine. The added amount of enzyme and au...

Embodiment 3

[0047] (1) Put the matrix pretreated by an atmospheric glycerol organic solvent (industrial glycerol was purchased from Wuxi Chemical Industry Station, with a purity ≥ 99.0%) in a pH 4.8 citric acid buffer solution;

[0048] (2) Add cellulase and compounded surfactant to (1); after enzymatic hydrolysis at 50° C. for 72 hours, obtain an enzymatic hydrolysis solution containing reducing sugars and perform detection.

[0049] Enzymolysis adopts the method of mechanical stirring and wet base (water content 50%) enzymolysis. The specific steps are as follows: the initial substrate concentration is 16%, feed 7%, 6% and 6% at 7h, 24h and 36h respectively, and the final substrate concentration is 35%.

[0050] In the above steps, the substrate concentration is 35%, the enzyme load is 3FPU / g, and the types and concentrations of the compounded surfactants are: 25mg / g Tween80, 8.5mg / g PEG4000, 8.5mg / g tea saponin, 27.7mg / g triton 100 and 8.5mg / g lauryl betaine. The added amount of enz...

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Abstract

The invention discloses a method for enhancing enzymolysis sugar generation of agricultural and forestry biomass raw material thick mash, and belongs to the technical field of enzyme engineering. Themethod includes the following steps of firstly, increasing the concentration of a medium to be 20-35% through a batched material supplementing mode; secondly, adding cellulase to the medium in the first step until the enzyme carrying capacity is 0.1-3 FPU / g; thirdly, assisting in the enzymolysis process through a compound surfactant, and making the concentration of fermentable sugar reach 150-230g / L after enzymolysis is conducted for 24-96 hours at 50 DEG C. The implementation of cellulose enzymolysis under the low enzyme carrying capacity and the compound surfactant is put forward for the first time, the fermentable sugar yield can be high, the production process is simple, production cost is greatly reduced, and the method is a feasible method for enzymolysis sugar generation through the agricultural and forestry biomass raw material thick mash.

Description

technical field [0001] The invention relates to a method for enzymatically hydrolyzing thick mash of agricultural and forestry biomass raw materials to produce sugar, and belongs to the technical field of enzyme engineering. Background technique [0002] With the increasingly prominent problems of resource and energy crisis, greenhouse effect and environmental pollution, biorefinery can fully replace bio-liquid fuels and bio-based chemicals produced by petrochemical production by using abundant agricultural and forestry cellulose biomass renewable resources. The current mainstream of the world's bioenergy industry. However, since the biorefinery technology of bioenergy and bio-based products from agricultural and forestry fiber biomass still faces many bottlenecks, especially the problem of enzymatic hydrolysis, the era of real biomass economy has not yet been realized. [0003] Cellulose molecules are connected together by hydrogen bonds to form a certain crystal structure...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/14C12P19/02
CPCC12P19/02C12P19/14
Inventor 孙付保陆梅杨林青
Owner JIANGNAN UNIV
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