Synthesis method of phosphorescence molecular probe for specific detection on calcium ions and application

A technology of phosphorescent molecules and synthesis methods, which is applied in the field of calcium ion fluorescent probes, can solve the problems of insufficient luminous intensity of probes and unsatisfactory calcium ion detection effects, and achieve excellent specific response, sensitive changes, and increased luminous intensity. Effect

Inactive Publication Date: 2019-08-30
ZHEJIANG NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The luminescence intensity of the probe for this invention application is not high enough, and in actual use, the detection effect of calcium ions is not ideal

Method used

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  • Synthesis method of phosphorescence molecular probe for specific detection on calcium ions and application
  • Synthesis method of phosphorescence molecular probe for specific detection on calcium ions and application
  • Synthesis method of phosphorescence molecular probe for specific detection on calcium ions and application

Examples

Experimental program
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Effect test

Embodiment 1

[0049] A method for synthesizing a phosphorescent molecular probe for specific detection of calcium ions, comprising the following steps:

[0050] Step 1, 200mg (the amount of substance is 0.75mmol) tetrachloroterephthalonitrile, 694mg (4.5mmol) 4-mercaptobenzoic acid and 1.66g (12.0mmol) potassium carbonate are added to a 100ml round bottom flask under nitrogen protection middle;

[0051] Step 2, add 10ml dry DMF to the round bottom flask to obtain the mixed solution;

[0052] Step 3, stirring the solution obtained in step 3 under heating conditions;

[0053] Heating temperature: 80°C; heating time: 48h;

[0054] Step 4: Add 30ml of hydrochloric acid with a concentration of 4M to the mixed solution obtained in step 3 to terminate the reaction, and obtain an orange precipitate as the target product.

[0055] The target product obtained in the preferred step 4 is recrystallized in ethanol after being separated by filtration and washed with distilled water, and dried in vacuo...

Embodiment 2

[0058] This embodiment discloses a phosphorescent molecular probe for the specific detection of calcium ions. The invention has aggregation-induced phosphorescence (AIP) characteristics and includes recognition units of cyano and carboxylic acid groups, and the recognition unit exhibits Excellent specific response.

Embodiment 3

[0060] A method for detecting calcium ion concentration using a phosphorescent molecular probe, comprising the following steps:

[0061] First, add the phosphorescent molecular probe and potassium hydroxide to the HEPES buffer solution with a pH value of 7.4 to prepare a phosphorescent molecular probe solution with a concentration of 0.5 mM;

[0062] Add Ca concentrations ranging from 0.0 to 500 μM to the phosphorescent molecular probe solution 2+ aqueous solution, with Ca 2+ The aqueous solution is added to the phosphorescent molecular probe solution to produce insoluble precipitates, and calcium ions trigger the aggregation of phosphorescent molecular probes to induce phosphorescence;

[0063] Use a xenon arc lamp to record the phosphorescence spectra corresponding to different calcium ion concentrations under 468nm excitation, and then obtain a standard line graph between phosphorescence intensity and calcium ion through linear fitting;

[0064] Add the aqueous solution o...

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Abstract

The invention discloses a synthesis method of a phosphorescence molecular probe for specific detection on calcium ions. The synthesis method comprises the following steps: I, putting tetrachloro-p-phthalonitrile, 4-sulfydryl benzoic acid and potassium carbonate into a round-bottom flask in the presence of nitrogen; II, adding dry DMF (dimethyl formamide) into the round-bottom flask so as to obtaina mixed liquid; III, stirring the solution obtained in the step III under a heating condition; and IV, adding hydrochloric acid into the mixed liquid obtained in the step III to terminate reactions,so as to obtain orange precipitate, namely a target product. The invention furthermore discloses a method and application for detecting calcium ions with the phosphorescence molecular probe. The phosphorescence molecular probe is simple in preparation process, high in calcium ion detection sensitivity, good in selectivity and accurate in quantitative detection.

Description

technical field [0001] The invention relates to the technical field of calcium ion fluorescent probes, in particular to a method for synthesizing a phosphorescent molecular probe for specific detection of calcium ions and the application of the probe in detecting calcium ion concentration and biological imaging. Background technique [0002] As a multi-party second messenger in plants, calcium ions participate in various regulatory processes, and play a vital role in various biological processes such as plant signal transduction, growth and development. At present, the acquisition of quantitative and spatial information of calcium ions in plants mainly relies on fluorescent molecular probes. Except for a small number of genetically encoded fluorescent protein probes, most fluorescent probes for calcium ion detection consist of small light-emitting organic molecules. This is due to their ease of synthesis and their bright fluorescence when bound to their target. However, mo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C323/62C07C319/14C09K11/06G01N21/64
CPCC07C319/14C07C323/62C09K11/06C09K2211/1007C09K2211/1014G01N21/6428G01N21/643
Inventor 钱兆生丰慧陈桂林
Owner ZHEJIANG NORMAL UNIVERSITY
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