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A kit for the visual detection of protamine based on palladium nanoperoxidase

A technology of peroxidase and kit, which is applied in the field of nanotechnology and biological detection, can solve the problems of insufficient detection sensitivity and complicated operation, and achieve the effects of high sensitivity, good selectivity and wide detection range

Active Publication Date: 2021-08-03
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many detection kits for protamine, but the operation is too complicated, and the detection sensitivity cannot meet the requirements [Talanta76(2008) 736-741; Analytical Chemistry 77(2005) 5221-5228; Journal of pharmaceutical and biomedical analysis 62(2012 )61–67]

Method used

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  • A kit for the visual detection of protamine based on palladium nanoperoxidase
  • A kit for the visual detection of protamine based on palladium nanoperoxidase
  • A kit for the visual detection of protamine based on palladium nanoperoxidase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Palladium nanoparticles having peroxidase activity are made of the following method:

[0028] 100 μl of 40 mM pH of a phosphate-phosphate bicycle solution, 12 μl of 100 mM tetrachloride aqueous solution, 48 μl of 0.5 mm heparinated aqueous solution, and the distilled water was added to 400 μl. The oscillation is mixed, 2 h is allowed to stand at 25 ° C, resulting in the reaction liquid, adding the newly prepared dimethylboron aqueous solution to the reaction liquid, 7 h at 25 ° C to obtain palladium nanoparticle synthesis having peroxidase activity Syndrome; Dimethyl Bolabane is DMAb; [DMAb] / [NA 2 PDCL 4 The molar ratio is 3.3.

[0029] The average particle diameter of 3.5 nm was obtained by transmitting electron microscopy scanning. (See figure 1 )

[0030] Peroxidase activity of palladium nanoparticles were determined:

[0031] 100 μl of Example 1 obtained with peroxidase activity of the palladium nanoparticle synthesis liquid in 1500 μl of centrifugal tube and added 90...

Embodiment 2

[0036] Palladium nanoparticles having peroxidase activity are made of the following method:

[0037] 200 μl of 20 mM pH of a phosphate-phosphate-phosphate solution, 120 μl of 10 mM tetrachloride aqueous solution, 12 μl of 2 mm heparinated aqueous solution of 12 μl of heparin, and added to distilled water to 400 μl The oscillation is mixed, and 3 h is allowed to stand at 20 ° C. The reaction liquid is obtained, and the newly prepared dimethylboronol aqueous solution is added to the reaction liquid, and 24 h is reduced at 20 ° C to obtain palladium nanoparticle synthesis of peroxidase activity. Syndrome; Dimethyl Bolabane is DMAb; [DMAb] / [NA 2 PDCL 4 The molar ratio is 3.3.

[0038] The average particle diameter of 3.1 nm is obtained by transmitting electron microscopy scanning.

[0039] The method of assaying the same example in Example 1, 3, 3 ', 5,5'-tetramethylene beaniline m The value is 0.045mm, which is K. hydrogen peroxide. m The value is 89.5mm.

Embodiment 3

[0041] Palladium nanoparticles having peroxidase activity are made of the following method:

[0042] 50 μl of 80 mM pH of a phosphate-phosphate buffered solution of 7.0, 10 μl of 120 mM tetrachloride aqueous sodium solution, 240 μL of 0.1 mm heparinated aqueous solution was added to 1.5 ml of centrifuge tube, and the distilled water was added to 400 μl, and the shaking mixed EtOAc (EtOAc) ((((((,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,, Dimethyl borogenic is DMAb; [DMAB] / [NA 2 PDCL 4 The molar ratio is 3.3.

[0043] The average particle diameter of 4.8 nm is obtained by transmitting electron microscopy scanning.

[0044] The method of assaying the same example in Example 1, 3, 3 ', 5,5'-tetramethylene beanilinem The value is 0.052mm, the K hydrogen peroxide m The value is 97.6mm.

[0045] Examples 1-3 Both the same kit were used to determine the palladium nanoparticles to 3, 3 ', 5, 5'-tetramethyleneaniline and hydrogen peroxide. m Value, summary is ...

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Abstract

The invention discloses a kit for visually detecting protamine based on palladium nanoparticles. The palladium nanoparticles with peroxidase activity are synthesized by a simple and environment-friendly liquid phase reduction method, and dihydrogen phosphate at pH 7.0 In the sodium-disodium hydrogen phosphate buffer solution, the addition of protamine can effectively inhibit the peroxidase activity of palladium, thereby changing the UV absorbance of the corresponding solution at 652nm, and plotting the concentration of protamine through the UV absorbance at 652nm , the linear equation between protamine concentration and absorbance is: y=-0.6806x+0.7388 and the linear correlation coefficient R 2 is 0.991, and then judge the concentration of added protamine by detecting the absorbance of the solution after adding protamine according to the linear equation. The kit of the invention can be conveniently used for the determination of protamine content in human serum and commercial products, and has the advantages of high sensitivity, good selectivity, wide detection range and the like.

Description

Technical field [0001] The present invention belongs to the field of nanotechnology and biological detection, and more particularly to a kit based on palladium nanoformase visualization to detect fish-free proteins. Background technique [0002] Fish essence is a low molecular protein with a large number of positive charges, mainly in the mature sperm nucleus of fish (such as squid, squid, etc.) as a nucleus and DNA binding. Fish protein is a strong base, which can have a stable salt to form a stable salt of strong heparin or heparin calcium, so it can be used for heparin injection of heparin injection, and spontaneously bleeding such as hemoptysis, It is the only drug in vitro circulating heart surgery against heparin. In addition, the fish extract has high nutrition and functionality, can lower blood pressure, help breathing, and promote digestion, etc., these also become the hotspots of research. There is currently a lot of detection kits for fish essence, but the operation is...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/33
CPCG01N21/33
Inventor 付雁黄青伟李韡张金利
Owner TIANJIN UNIV