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Modified extracellular matrix hydrogel and its preparation method and application, tissue engineering material

A tissue engineering and extramatrix technology, used in pharmaceutical formulations, prostheses, drug delivery, etc., can solve problems such as easy degradation, inability to form gels, poor treatment effects, etc., to achieve wide sources, maintain mechanical strength, good Effects of Cell Affinity Properties

Active Publication Date: 2020-06-30
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Extracellular matrix hydrogels can be transplanted into spinal cord injury sites to promote cell and tissue regeneration. However, due to the soft texture of natural extracellular matrix hydrogels, they cannot be shaped into gels in vivo, and they are easily degraded after implantation, resulting in therapeutic not effectively

Method used

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  • Modified extracellular matrix hydrogel and its preparation method and application, tissue engineering material
  • Modified extracellular matrix hydrogel and its preparation method and application, tissue engineering material
  • Modified extracellular matrix hydrogel and its preparation method and application, tissue engineering material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] The preparation of the decellularized matrix hydrogel derived from spinal cord comprises the following steps:

[0049] The fresh spinal cord of pigs obtained from the slaughterhouse, under low temperature conditions, peel off the outer membrane of the spinal cord and cut it into 1.5-2cm length, put it into the pre-cooled phosphate buffer saline (PBS) solution, PBS and put into The volume ratio of the spinal cord is about 4:1; then, under the condition of ice bath, put the screw bottle containing the spinal cord on a decolorization shaker, and shake it at a speed of 160rpm for at least 4 hours; filter the excess liquid with gauze, Then put the remaining spinal cord structure into the screw bottle, add double distilled water to 500mL, put it into the shaker again, and repeat this process until only the transparent cytoskeleton remains in the spinal cord. This process needs to go through 7 to 8 cycles. Fully remove the gray matter, white matter and cytoplasmic components i...

Embodiment 2

[0051] The preparation of PLLA electrospinning nanofiber membrane containing amide bond comprises the following steps:

[0052] Weigh a certain amount of poly-L-lactic acid (PLLA) powder, add 2 mL of hexafluoroisopropanol (HFIP) and stir for 12 h to fully dissolve PLLA and obtain a PLLA solution with a mass concentration of 7.5%. Inject the solution prepared above into a 5mL glass syringe, install a 20g stainless steel needle and install it on the electrospinning device, take a flat tinfoil paper and fix it on the receiving end, adjust the receiving distance and set a suitable Under high voltage and flow rate, the PLLA solution forms oriented fiber filaments under high pressure and is received on the receiving plate of tin foil paper, and the ambient temperature is controlled at 30 °C. The process parameters for preparing PLLA spinning fiber bundles are as follows: the spinning voltage is 15kV, the distance between the spinning needle and the receiver is 30cm, and the spinning...

Embodiment 3

[0054] The preparation of PLA electrospinning nanofiber membrane containing amide bond comprises the following steps:

[0055] Weigh an appropriate amount of polylactic acid (PLA) powder, add 3mL N,N-dimethylformamide (DMF) and let it stand for 8 hours to fully dissolve PLA and obtain a PLA solution with a mass concentration of 8.0%. Inject the solution prepared above into a 5mL glass syringe, install a 20g stainless steel needle and install it on the electrospinning device, take a flat tinfoil paper and fix it on the receiving end, adjust the receiving distance and set a suitable Under high voltage and flow rate, the PLLA solution forms oriented fiber filaments under high pressure and is received on the receiving plate of tin foil paper, and the ambient temperature is controlled at 30 °C. The process parameters for preparing PLA spinning fiber bundles are as follows: the spinning voltage is 15kV, the distance between the spinning needle and the receiver is 30cm, and the spinn...

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Abstract

The invention provides modified extracellular matrix hydrogel, a preparation method and application thereof, and a tissue engineering material, and relates to the technical field of hydrogels. The modified extracellular matrix hydrogel is made mainly by mixing extracellular matrix hydrogel and nanofibers which hyaluronic acid is grafted to; the hyaluronic acid-modified nano fibers have good biocompatibility and good cellular affinity with the extracellular matrix hydrogel; the nanofibers may act as a framework to the extracellular matrix hydrogel in order to enhance mechanical properties of the extracellular matrix hydrogel; the problem can be solved that the prior art lacks a hydrogel having good mechanical properties and good biocompatibility and bioactivity.

Description

technical field [0001] The invention relates to the field of hydrogel technology, in particular to a modified extracellular matrix hydrogel, a preparation method and application thereof, and a tissue engineering material. Background technique [0002] Hydrogel is a high molecular polymer with superior mechanical properties. Its internal porous network structure is conducive to the attachment and migration of cells. It is rich in water and can be filled in the defect when it is in a liquid state. The effect of body temperature Form a semi-solid colloid, thereby reducing the secondary damage caused after implantation. [0003] Extracellular matrix (extracellular matrix, ECM) is a biological network structure composed of macromolecules. It is a natural biologically derived material after removing cellular components in biological tissues through physical and chemical methods. The main components are collagen, non-collagen, and elastin. , proteoglycans, aminoglycans and some bi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/52A61L27/36A61L27/50A61L27/20A61L27/58C08J3/075C08L67/04C08L5/08
CPCA61L27/20A61L27/3633A61L27/50A61L27/52A61L27/58A61L2400/12C08J3/075C08J2367/04C08J2405/08C08L5/08
Inventor 全大萍李锐邓荣力张驰白莹
Owner SUN YAT SEN UNIV
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