A method for detecting n-dimethylnitrosamine in meat food

Abstract

The invention provides a method for detecting N-dimethylnitrosamine in meat food, which uses porous magnetic adsorption material to adsorb N-dimethylnitrosamine in meat food, and realizes N-dimethylnitrosamine in combination with liquid-liquid extraction method. The high recovery rate and detection accuracy of dimethylnitrosamine, based on the method of high-performance liquid chromatography and mass spectrometry, the accuracy and low cost of N-dimethylnitrosamine detection are realized, and the The advantages of high extraction efficiency, less solvent use, short time consumption, and less loss of N-dimethylnitrosamine in fat samples can greatly improve the detection accuracy of N-dimethylnitrosamine in meat products. improvement.

Description

technical field [0001] The invention belongs to the technical field of food detection, and more specifically relates to a method for detecting N-dimethylnitrosamine in meat food. Background technique [0002] N-Dimethylnitrosamine (NDMA) is a semi-volatile organic chemical widely present in various pickled, smoked and grilled meat products and heat-processed foods. It is rapidly absorbed through the digestive tract and respiratory tract, and slowly absorbed through the skin. , mainly causing liver damage, which is extremely harmful to the human body. The national standard GB2762-2017 "National Food Safety Standard Contaminant Limits in Food" has clear limit regulations on NDMA, and the maximum limit of NDMA in meat products (except canned meat) is 3 μg / Kg. [0003] At present, the detection methods of N-dimethylnitrosamine mainly include gas chromatography-thermal energy analyzer method, gas chromatography-nitrogen phosphorus detector, gas chromatography-mass spectrometry, ...

AI Technical Summary

Problems solved by technology

[0003] At present, the detection methods of N-dimethylnitrosamine mainly include gas chromatography-thermal energy analyzer method, gas chromatography-nitrogen phosphorus detector, gas chromatography-mass spectrometry, gas chromatography-tandem mass spectrometry or liquid chromatography-tandem mass spectrometry However, thermal energy analyzers are expensive, have a narrow application range, and are not very adaptable. They are only limited to the analysis of N-nitrosamine compounds, which are not equipped in general laboratories, and the daily maintenance of the detector is troublesome. This also limits its wide application, and due to the low-molecular properties of nitrosamines, the signal will be seriously interfered by the background noise signal of the mass spectrometer; the gas chromatography-nitrogen and phosphorus detector has a high degree of automation and high sensitivity, and can be used for volatile N - Determination of nitrosamine compounds, but this method is prone to false positive results. It can be seen that seeking rapid and sensitive detection methods is still a major challenge in this field

[0004] In addition, food, especially meat products, has high oil content, complex matrix, and low content of target substances, which increase the difficulty of extraction, and the extraction of analytes is also the key to detection. For this reason, many sample pretreatment techniques have been applied to N-dimethylformaldehyde The extraction of nitrosamines mainly includes steam distillation, solvent extraction, vacuum distillation, or solid phase extraction, etc., but the traditional extraction methods have cumbersome operations, consume a large amount of organic solvents, consume a large amount of samples, and have low recovery rates. Unstable and not conducive to batch processing and other shortcomings, which severely limit their daily application

For example, CN2013103497558 discloses a method for the rapid determination of dimethylnitrosamine in meat products by ultrasonic extraction. The method is simple and time-consuming, but lacks sufficient purification means. The concentration step is difficult to carry out. In addition, because the main damage step of N-dimethylnitrosamine in the experimental process is the concentration step, the existing concentration methods are likely to cause large damage to N-dimethylnitrosamine and decrease the accuracy.

Images