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Streptomyces hydrogenans and application thereof in biological control

A technology of Streptomyces hydrangeum and Streptomyces hydrangeum, which is applied in the field of Streptomyces hydrangeum, can solve the problems of lack of biological pesticides, species and quantity cannot meet agricultural production, etc., achieve good social and economic benefits, protect and promote root growth, and enhance resistance effect of ability

Inactive Publication Date: 2019-09-10
XUZHOU NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of new antibiotics such as Jinggangmycin, Nongkang 120, Zhongshengmycin, Wuyimycin and Liuyangmycin derived from microbial metabolites has promoted the development of biological control of agricultural diseases to a certain extent. Pesticides are far from meeting the needs of agricultural production both in terms of type and quantity.
According to statistics, at present, my country's biopesticides only account for 5% to 7% of the market, and many major plant diseases lack corresponding biopesticides.

Method used

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  • Streptomyces hydrogenans and application thereof in biological control
  • Streptomyces hydrogenans and application thereof in biological control
  • Streptomyces hydrogenans and application thereof in biological control

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A strain KLBMP-3095 was obtained by collecting eucalyptus rhizosphere soil at Dananshan Forest Farm in Zhaoqing City, Guangdong Province, and was isolated and purified by conventional methods. The strain grows better on Cha's medium, yeast extract powder-malt extract agar medium (ISP2) and glycerol asparagine agar medium. The aerial hyphae and basal hyphae grow luxuriantly, the spore chains formed by mature sporocydia are in the shape of beads, the spores are cylindrical, and the surface is smooth without spines, which has the typical morphological characteristics of Streptomyces ( figure 1 ). Determine the 16S rRNA sequence (SEQ ID No.1) of the strain, compare this sequence with the effective strains in the EzTaxon database, construct a phylogenetic tree with MEGA5.01 software, combine the morphology and culture characteristics, physiological and biochemical characteristics, It was identified as a strain of Streptomyces hydrogenans.

Embodiment 2

[0030] Embodiment 2 Bacterial strain plate face-off experiment

[0031] Pick the Alternaria solani stored in the inclined tube and inoculate it on one side of the PDA plate, and at the same time inoculate the strain KLBMP-3095 on the other side, and culture it in a constant temperature incubator at 28°C. Repeat three times, observe and record the growth status of the two bacteria on the plate. as attached figure 2 shown. It can be seen from the figure that the mycelium of Alternaria solani can not grow close to the strain KLBMP-3095, indicating that the strain KLBMP-3095 can effectively inhibit the growth of Alternaria solani in the plate experiment.

Embodiment 3

[0032] Embodiment 3 Growth Rate Method Measures Fermentation Liquid Inhibits Alternaria Solani Growth Experiment

[0033] In the ultra-clean workbench, pick an appropriate amount of KLBMP-3095 bacteria from the seed tube for activation, and inoculate the activated bacteria into the liquid medium.

[0034] The composition of the liquid fermentation medium is: 4g of sucrose, 10g of malt extract powder, 4g of beef extract, 1000mL of distilled water, and the pH is 8.0.

[0035] 28°C, 150r / min, culture for 5 days, until the bacteria grow to the logarithmic growth phase, stop the fermentation. The fermentation product was centrifuged at 7800 r / min for 10 min, and the supernatant was filtered through a 0.22 μm sterile filter membrane to obtain a sterile fermentation broth. When the PDA medium is autoclaved and cooled to 60°C, mix according to the ratio of medium: sterile fermentation broth = 9:1 and pour onto the plate. The bacterium cake (diameter 4mm) of the pathogenic bacteria c...

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Abstract

The invention discloses a strain of streptomyces hydrogenans and application thereof in biological control. The preservation number of the streptomyces hydrogenans is CGMCC No. 17449. The applicationof the streptomyces hydrogenans specifically refers to application in control over alternaria solani, and includes the steps that streptomyces hydrogenans thalli are activated, fermentation culture iscarried out until the thalli grow to the logarithmic phase, fermentation is stopped, and the obtained fermentation product is a biocontrol agent; the obtained biocontrol agent is diluted, and root dipping is carried out with the diluent when plant seedlings are transplanted and / or root irrigation is carried out with the diluent when seedlings grow to the 4-5 leaf stage. The application of the streptomyces hydrogenans can replace chemical pesticides to effectively control alternaria solani diseases. The biocontrol agent contains growth-promoting components and beneficial microorganisms, and can effectively protect and promote the root growth and enhance the resistance of the root system to adverse environments and harmful bacteria; the agent also contains components capable of inhibiting and killing alternaria solani and can effectively control plant fungal diseases caused by alternaria solani.

Description

technical field [0001] The present invention relates to microorganisms and applications thereof, in particular to a strain of Streptomyceshydrogenans [0002] CGMCC No.17449 and its application in biological control. Background technique [0003] Alternaria Solani (Ellis et Martin) Jones et grout is one of the fungi that are easy to infect plants and cause plant diseases. . The fungus can cause many important crop diseases, such as potato early blight, pear black spot, cabbage black spot, tobacco red spot, apple leaf spot, tomato early blight, etc., seriously affecting the production of these economic crops. [0004] At present, the method of preventing and controlling the diseases caused by Alternaria solani mainly uses chemical pesticides. Although chemical pesticides have the advantages of wide adaptability, simple operation, rapid action, significant effect, and emergency response, etc.; however, chemical pesticides are a double-edged sword. They promote agricultural ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00A01G13/00A01G7/06A01G22/05C12R1/465
CPCA01G7/06A01G13/00A01N63/00A01G22/05C12N1/205C12R2001/465
Inventor 鞠秀云姜素平
Owner XUZHOU NORMAL UNIVERSITY
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